Fluorescein isothiocyanate conjugated anti mouse igg

Manufactured by Jackson ImmunoResearch

Sourced in United States

Fluorescein isothiocyanate-conjugated anti-mouse IgG is a laboratory reagent that consists of an antibody specific to mouse immunoglobulin G (IgG) molecules, which has been conjugated with the fluorescent dye fluorescein isothiocyanate (FITC). This reagent is used to detect and visualize the presence of mouse IgG in various biological samples and experimental procedures.

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Lab products found in correlation

Imagem, by Hamamatsu Photonics (2 mentions) Anti pcna, by Proteintech (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Rabbit anti h3k27me3 antibody, by Merck Group (1 mentions) Goat anti oct4 antibody, by Santa Cruz Biotechnology (1 mentions) Anti 5mc, by Eurogentec (1 mentions) Rhodamine conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Imagem emccd camera, by Hamamatsu Photonics (1 mentions) Anti 5hmc, by Active Motif (1 mentions) Lsm 800, by Zeiss (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Axiovision software, by Zeiss (1 mentions) Alexa flour 568 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Rabbit anti h3k9me3, by Merck Group (1 mentions) Vectashield anti bleaching solution with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions) Csu10, by Yokogawa (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Biotium (1 mentions)

Spelling variants of this product

Fluorescein isothiocyanate-conjugated goat anti-mouse IgG (6 citations) Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (4 citations) Anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC) (2 citations)

6 protocols using fluorescein isothiocyanate conjugated anti mouse igg

Lung Tissue Histological Analysis in Mice

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The right lung of each mouse was sampled, fixed in 4% paraformaldehyde, and processed using standard techniques by staining sections of paraffin-embedded tissue for H&E, immunohistochemistry, and immunofluorescence. For immunohistochemistry, the primary antibody rabbit polyclonal anti-PCNA (Proteintech, Rosemont, IL, USA) was used. For Wnt5a immunofluorescence staining, sections of mouse lungs were deparaffinized, rehydrated, and subjected to heat-induced epitope retrieval. After washing and blocking, the slides were incubated overnight with mouse anti-Wnt5a primary antibody at 4°C. On the following day, incubation with fluorescein isothiocyanate–conjugated antimouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) took place for 1 hour in the dark at room temperature. Sections were subsequently washed three times, and DAPI nuclear stain was applied for fluorescence microscopy. For dual immunofluorescence staining, primary antibodies, including antineutrophils (mouse Ly6G; Abcam, Cambridge, UK) and T cells (rabbit CD3; Proteintech), were used to assess inflammatory cell populations.

Wang Z., Zhao J., Wang T., Du X, & Xie J. (2019). Fine-particulate matter aggravates cigarette smoke extract–induced airway inflammation via Wnt5a–ERK pathway in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 14, 979-994.

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Immunofluorescence Analysis of Blastocyst Lineage Markers

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Blastocysts were fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature. After washing with PBS containing 10 mg/ml bovine serum albumin (BSA; Merck # 12657) (PBS/BSA), the fixed embryos were permeabilized by 15 min incubation with 0.5% Triton X-100. After blocking in PBS/BSA for 1 h at room temperature, they were incubated in a mixture of primary antibodies including rabbit anti-H3K27me3 antibody (1/500, Millipore # 07-449), goat anti-Oct4 antibody (1/500, SantaCruz # sc-8628) and mouse anti-Cdx2 antibody (1/100, BioGenex # AM392-5M) at 4°C overnight. Following three washes with PBS/BSA, the embryos were incubated with a mixture of secondary antibodies including fluorescein isothiocyanate-conjugated anti-mouse IgG (1/400, Jackson Immuno-Research), Alexa Flour 546 donkey anti-rabbit IgG (1/400, Thermo Fisher Scientific) and Alexa Flour 647 donkey anti-goat IgG (1/400, Thermo Fisher Scientific) for 1 h at room temperature. Finally, they were mounted with Vectashield with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories # H-1200). The fluorescent signals were observed using a laser-scanning confocal microscope (Zeiss LSM510) and an EM-CCD camera (Hamamatsu ImagEM). Three to five embryos were examined for each condition.

Matoba S., Wang H., Jiang L., Lu F., Iwabuchi K.A., Wu X., Inoue K., Yang L., Press W., Lee J.T., Ogura A., Shen L, & Zhang Y. (2018). Loss of H3K27me3 Imprinting in Somatic Cell Nuclear Transfer Embryos Disrupts Post-Implantation Development. Cell stem cell, 23(3), 343-354.e5.

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Immunohistochemical analysis of 5mC and 5hmC

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Growing oocytes collected at postnatal day 7 were fixed in 3.7% paraformaldehyde in PBS for 20 min, washed with PBS containing 0.1% BSA, permeabilized with 0.5% Triton X-100 for 15 min. The cells were denatured with 4 N HCl for 10 min, neutralized with 100 mM Tris-HCl (pH 8.5) for 20 min, and then incubated with 1/500 anti-5mC (Eurogentec) and 1/500 anti-5hmC (Active Motif) primary antibodies for 1 h at room temperature. After washing with in PBS with BSA, the cells were incubated with 1/250 fluorescein isothiocyanate-conjugated anti-mouse IgG (Jackson Immuno-Research) and 1/250 rhodamine-conjugated anti-rabbit IgG (Jackson Immuno-Research) for 1 h. The oocytes were then mounted on a glass slide in VECTASHEILD medium with DAPI (Vector Laboratory) and observed under a CSU-10 confocal laser scanning microscope (Yokogawa) with an ImagEM EM-CCD camera (Hamamatsu).

Toriyama K., Au Yeung W.K., Inoue A., Kurimoto K., Yabuta Y., Saitou M., Nakamura T., Nakano T, & Sasaki H. (2024). DPPA3 facilitates genome-wide DNA demethylation in mouse primordial germ cells. BMC Genomics, 25, 344.

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Immunolabeling of Zygotic H3K27me3 and H3K9me3

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Zygotes were fixed in 3.7% paraformaldehyde (PFA) in PBS containing 0.2% Triton for 20 min. After 4x washes with PBS containing 10 mg/ml BSA (PBS/BSA), zygotes were treated with primary antibodies at 4°C overnight. The primary antibodies used in this study were mouse-anti-H3K27me3 (1/500, Active Motif, 61017), rabbit anti-H3K9me3 (1/500, Millipore, 07-442), and rabbit anti-FLAG (1/2000, Sigma-Aldrich, F7524). After 3x washes with PBS/BSA, samples were incubated with a 1:250 dilution of fluorescein isothiocyanate–conjugated anti-mouse IgG (Jackson Immuno-Research) or Alexa Flour 568 donkey anti-rabbit IgG (Life technologies) for 1 h. The zygotes were then mounted on a glass slide in Vectashield anti-bleaching solution with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). Fluorescence was detected under a laser-scanning confocal microscope with a spinning disk (CSU-10, Yokogawa) and an EM-CCD camera (ImagEM, Hamamatsu) or Zeiss LSM800.
All images were acquired and analyzed using the Axiovision software (Carl Zeiss). The fluorescent signal intensity was quantified with the Axiovision software. Briefly, the signal intensity within the maternal pronuclei was determined, and the cytoplasmic signal was subtracted as background. Then, the averaged signal intensity of the no-injection control zygotes was set as 1.0.

Inoue A., Jiang L., Falong L., Suzuki T, & Zhang Y. (2017). Maternal H3K27me3 controls DNA methylation-independent genomic imprinting. Nature, 547(7664), 419-424.

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Immunocytochemical Characterization of PDLSCs

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PDLSC identity was confirmed by immunocytochemical staining by using the mouse antibodies against rabbit vimentin and keratin (Boster Biological Technology Ltd., Wuhan, China) as well as human STRO-1 (R&D Systems, Inc., Minneapolis, MN, USA) at room temperature for 2 hours. Cells were washed with phosphate-buffered saline (PBS) and incubated with the fluorescein isothiocyanate-conjugated anti-mouse IgG (1:100; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 1 hour at 37°C and then counterstained with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (Biotium, Hayward, CA, USA). The cells were rinsed three times with PBS and analyzed by using a DMIL fluorescent-inverted phase-contrast microscope equipped with a Leica MPS-30 camera (Leica, Bensheim, Germany).

Su F., Liu S.S., Ma J.L., Wang D.S., E L.L, & Liu H.C. (2015). Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells. Stem Cell Research & Therapy, 6(1), 22.

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Cardiac Fibrosis and Estrogen Receptor Imaging

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LV sections were stained with Sirius-red to determine collagen deposition as previously described [18] (link). Fibrosis in remote area was calculated as the ratio of fibrotic area (excluding the MI zone) to total myocardial area in the remote zone. For immunofluorescence, paraffinembedded sections were incubated with anti-mouse ER antibody (Acris SP5198P) followed by secondary Fluorescein isothiocyanate-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories), as described before [7] (link). Nuclei were stained with 6-

Schuster I., Mahmoodzadeh S., Dworatzek E., Jaisser F., Messaoudi S., Morano I, & Regitz-Zagrosek V. (2016). Cardiomyocyte-specific overexpression of oestrogen receptor β improves survival and cardiac function after myocardial infarction in female and male mice. Clinical science (London, England : 1979), 130(5).

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