Two weeks before the mice were sacrificed for histoimmunostaining or histochemistry analysis, WGA overexpressing lentivirus (5 µL at 109/mL, the construction data of Lentivirus-WGA refer to Supplementary Fig. 4 ) were injected into the somatosensory area through a glass micropipette and stereotaxic injector (KDS310, Muromachi-Kikai).
Kds 310
Manufactured by Muromachi Kikai
Sourced in Japan
The KDS 310 is a laboratory syringe pump designed for accurate and consistent fluid delivery. It features a microprocessor-controlled stepper motor that can accommodate syringes ranging from 0.5 to 60 mL in volume. The pump can deliver flow rates from 0.001 to 170.9 mL/min, depending on the syringe size. The device includes an LCD display and intuitive controls for easy programming and operation.
Automatically generated - may contain errors
Lab products found in correlation
Ih impactor, by Precision Systems and Instrumentation (3 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Infinite horizon impactor, by Precision Systems and Instrumentation (1 mentions)
Digigait system, by Mouse Specifics (1 mentions)
Mrs 2179, by Abcam (1 mentions)
Nod scid mice, by Oriental Yeast (1 mentions)
17 protocols using kds 310
1
Lentiviral WGA Overexpression in Somatosensory Cortex
2
Immunomodulatory Effects of IFN-γ and LPS in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Injured mice received an injection of 2 μg of recombinant murine IFN-γ (PeproTech) and 200 ng of LPS (Sigma-Aldrich) at the epicenter using a stereotaxic injector (KDS 310, Muromachi Kikai) every 2 days from 2 dpi. For in vitro combination administration, 10 ng of IFN-γ and 100 ng of LPS were added to macrophages (4.0 × 104/ml) differentiated from WT CD11b+/Ly6G−/CD45high peripheral blood–derived monocytes after cultivated for 4 days as previously described (59 (link)), and an immunocytochemical analysis and mRNA extraction were performed at 3 hours after stimulation, respectively. The paw withdrawal threshold was analyzed as previously described (19 (link)).
3
Transplantation of Macrophages in Spinal Cord Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using flow cytometry, macrophages with or without IRF8 activation were obtained from the injured spinal cord of bone marrow chimeric mice [EGFP+ macrophages: IRF8+/+]/[microglia: IRF8+/+] at 7 or 4 dpi. EGFP+ cells (5.0 × 105) were transplanted into the injured spinal cord of WT mice immediately after SCI using a glass micropipette and a stereotaxic injector, as described previously (KDS 310, Muromachi Kikai) (6 (link)). Injections were performed bilaterally at four sites of intact parenchyma 1.5 mm rostral and caudal to the epicenter.
4
Contusive Spinal Cord Injury and 253G1-NSs Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult female NOD-SCID mice (20–22 g) were anesthetized via intraperitoneal (i.p.) injection of ketamine (100 mg/kg) and xylazine (10 mg/kg). After laminectomy, contusive SCI was induced at the Th10 level using an IH impactor (60 kdyn; Precision Systems and Instrumentation) as described previously (Scheff et al., 2003 (link)). Nine days after SCI, 253G1-NSs (5 × 105 cells) were transplanted into the lesion epicenter of each mouse (n = 37) using a glass micropipette and stereotaxic injector (KDS310; Muromachi Kikai). The 253G1-NSs were transplanted at approximately the same time as the PBS injection and 201B7-NS transplantation described in our previous report (Nori et al., 2011 (link)).
5
Transplanted hADSC-NCs for MCAO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To obtain the transplanted hADSC-NCs, hADSCs were first labeled with an EGFP-expressing lentivirus or mCherry-TK-overexpressing lentivirus. Forty-eight hours after infection, hADSCs were observed under a fluorescence microscope at wavelengths of 488 nm and 568 nm. A percentage of hADSCs labeled with green fluorescence of 80–90% indicated successful infection. Then, the labeled hADSCs were incubated with the aforementioned modified cocktail [20 , 61 (link)], and induced to differentiate into neuron-like cells for 24 h. hADSC-NCs were collected and resuspended at a final concentration of 1 × 109 cells/ml in PBS. Five microliters of this cell suspension were injected into the MCAO mouse brain injury lesion through a glass micropipette and stereotaxic injector (KDS310, Muromachi-Kikai). The detailed procedure was similar to our previously published procedure [72 (link)].
6
iPSC-NSPC Transplantation for Spinal Cord Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female 8-week-old C57BL6/J mice and BALB/cA mice (20–22 g) were anesthetized with an intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg/kg). After laminectomy at the 10th thoracic spinal vertebra, the dorsal surface of the dura mater was exposed. Contusive spinal cord injury was induced at the Th10 level using an infinite horizon impactor (60 kdyn; Precision Systems and Instrumentation, Lexington, KY, USA). Nine days after injury, 5 × 105 iPSC-NSPCs were transplanted into the lesion epicenter using a glass micropipette at a rate of 1 µL/min with a 25-µL Hamilton syringe and stereotaxic microinjector (KDS 310, Muromachi Kikai Co., Ltd, Tokyo, Japan). All surgeries were performed under anesthesia, and all efforts were made to minimize animal suffering.
7
Tracing Exogenous hADSCs in MCAO Brain Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To trace exogenously introduced hADSCs in the MCAO brain, enhanced GFP (EGFP) expressing lentivirus FG12 was packaged and infected into hADSCs before injection. hADSCs were observed 48 hours after infection under a fluorescent microscope at a wavelength of 488 nm. A total of 80% to 90% percent of hADSCs labeled with green fluorescence was regarded as successful infection. Then, EGFP-hADSCs were collected and adjusted to a final concentration of 1 × 109 cells/ml suspended in PBS. An amount of 5 μl of this cell suspension was injected into the MCAO mouse brain injury area through a glass micropipette and stereotaxic injector (KDS310, Muromachi-Kikai). Briefly, we injected 5 μl cell suspension from three directions within one pole at the infarct lesion. This injection process took six minutes. After injection, the syringe was kept in the brain tissue for one minute to achieve good absorption. In parallel, 5 μl of PBS was injected into the control MCAO group of mice. For the sham group, nothing was done.
8
Targeted C5a Injection in Spinal Cord Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A glass tip was inserted 2 mm rostral and caudal from the epicenter of the injured spinal cord, and 2 μl of 1 μM mouse recombinant C5a (R&D Systems) was injected at 0.5 μl/min using a stereotaxic injector (KDS 310, Muromachi Kikai) from 4 to 7 dpi (14 (link)). Control animals received 2 μl of PBS from 4 to 7 dpi.
9
Comprehensive Evaluation of Hindlimb Motor Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hindlimb motor function of each mouse was evaluated weekly using the Basso mouse scale (BMS) up to 42 d after injury (Basso et al., 2006 (link)). Two persons blinded to the mouse group performed the behavioral analyses. At 42 d after injury, motor function was also assessed on a rotating rod apparatus (KDS310; Muromachi-Kikai Co., Ltd.) by measuring the amount of time that mice could remain on the rod while it rotated at 10 rotations per minute (rpm). A treadmill gait analysis was performed using the DigiGait System (Mouse Specifics). The stride lengths and stance angles of the hindlimbs were measured on a treadmill at a speed of 6 cm/s, and phase dispersion was analyzed in the DigiGait analyses to provide an indication of limb coordination.
10
Modulating OASIS Expression After SCI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five days after SCI, animals were anesthetized via sustained inhalation of 2.0% sevoflurane and 2.0 L/min air prior to the injection of siRNA (12.5 µM) into the injured spinal cord. After exposing the T10 level of the spinal cord, 2 µL of anti-OASIS siRNA 5′-GAA AUG AGC CAG UUU CUC AdTdT-3′ (sense) and 5′-UGA GAA ACU GGC UCA UUU CdTdT-3′ (antisense) (Kondo et al., 2005) (OASIS siRNA group) or scrambled SiRNA (scrambled siRNA group) mixed with peptide transduction domain-double strand RNA binding domain (PTD-DRBD) were injected (2 µL/min) into the center and periphery of the injured lesion using a 32-gauge needle and stereotaxic injector (KDS310; Muromachi Kikai, Tokyo, Japan). Two days later, mice were anesthetized using sustained inhalation of 2.0% sevoflurane and 2.0 L/min air prior to harvesting the injured spinal cords. The spinal cord samples were also harvested from untreated SCI mice (control group) at day 7 after SCI. The mRNA and protein expression of OASIS was assessed using the above mentioned real-time PCR and western blot analysis (n = 5 per group).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!