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Zen 880 microscope

Manufactured by Zeiss
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The Zeiss Zen 880 is a high-performance microscope designed for advanced imaging and analysis. It features advanced optics, a sophisticated control system, and a modular design to support a wide range of applications.

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Lab products found in correlation

Zen black, by Zeiss (3 mentions) Donkey anti rabbit 488, by Thermo Fisher Scientific (3 mentions) Prolong diamond, by Thermo Fisher Scientific (3 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions) Sucrose, by Merck Group (3 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

3 protocols using zen 880 microscope

1

Imaging Thymic Niche Cell Subsets

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Thymus tissue from Foxn1Cre/Rosa26YFP/Cxcl12 mice was isolated and fixed in 2% paraformaldehyde (PFA; Sigma) for 2 h, then overnight in 15% sucrose (Sigma). Thymic lobes were frozen on dry ice and sectioned at 7 μm within 24 h of freezing. eYFP protein in sections from Foxn1Cre/Rosa26YFP/Cxcl12DsRed was amplified using rabbit anti-GFP (ThermoFisher) and donkey anti-rabbit 488 (ThermoFisher). Sections were counterstained with DAPI (Sigma) and mounted using Prolong Diamond (ThermoFisher). Sections were imaged using Zeiss Zen 880 microscope (Zeiss) and analysis using Zeiss Zen Black (Zeiss).
White A.J., Parnell S.M., Handel A., Maio S., Bacon A., Cosway E.J., Lucas B., James K.D., Cowan J.E., Jenkinson W.E., Hollander G.A, & Anderson G. (2023). Diversity in Cortical Thymic Epithelial Cells Occurs through Loss of a Foxn1-Dependent Gene Signature Driven by Stage-Specific Thymocyte Cross-Talk. The Journal of Immunology Author Choice, 210(1), 40-49.
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2

Imaging Thymic Niche Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thymus tissue from Foxn1Cre/Rosa26YFP/Cxcl12 mice was isolated and fixed in 2% paraformaldehyde (PFA; Sigma) for 2 h, then overnight in 15% sucrose (Sigma). Thymic lobes were frozen on dry ice and sectioned at 7 μm within 24 h of freezing. eYFP protein in sections from Foxn1Cre/Rosa26YFP/Cxcl12DsRed was amplified using rabbit anti-GFP (ThermoFisher) and donkey anti-rabbit 488 (ThermoFisher). Sections were counterstained with DAPI (Sigma) and mounted using Prolong Diamond (ThermoFisher). Sections were imaged using Zeiss Zen 880 microscope (Zeiss) and analysis using Zeiss Zen Black (Zeiss).
White A.J., Parnell S.M., Handel A., Maio S., Bacon A., Cosway E.J., Lucas B., James K.D., Cowan J.E., Jenkinson W.E., Hollander G.A, & Anderson G. (2023). Diversity in Cortical Thymic Epithelial Cells Occurs through Loss of a Foxn1-Dependent Gene Signature Driven by Stage-Specific Thymocyte Cross-Talk. The Journal of Immunology Author Choice, 210(1), 40-49.
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3

Imaging Thymic Stromal Cells in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thymus tissue from Foxn1Cre/Rosa26YFP/Cxcl12 mice was isolated and fixed in 2% PFA (Sigma) for 2 hours, then overnight in 15% Sucrose (Sigma). Thymic lobes were frozen on dry ice, and sectioned at 7μm within 24 hours of freezing. eYFP protein in sections from Foxn1Cre/Rosa26YFP/Cxcl12DsRed was amplified using rabbit anti-GFP (ThermoFisher) and donkey anti-rabbit 488 (ThermoFisher). Sections were counter stained with DAPI (4’,6-diamidino-2-phenylindole)(Sigma) and mounted using Prolong Diamond (ThermoFisher). Sections were imaged using Zeiss Zen 880 microscope (Zeiss) and analysis using Zeiss Zen Black (Zeiss).
White A.J., Parnell S.M., Handel A., Maio S., Bacon A., Cosway E.J., Lucas B., James K.D., Cowan J.E., Jenkinson W.E., Hollander G.A, & Anderson G. (2023). Diversity in Cortical Thymic Epithelial Cells Occurs through Loss of a Foxn1-Dependent Gene Signature Driven by Stage-Specific Thymocyte Cross-Talk. The Journal of Immunology Author Choice, 210(1), 40-49.
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