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Eriocitrin

Manufactured by Yuanye Bio-Technology
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Sourced in China

Eriocitrin is a flavonoid compound extracted from the citrus fruit. It serves as a key ingredient in various laboratory research applications.

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Lab products found in correlation

Naringin, by Yuanye Bio-Technology (6 mentions) Naringenin, by Yuanye Bio-Technology (6 mentions) Narirutin, by Yuanye Bio-Technology (5 mentions) Tangeretin, by Yuanye Bio-Technology (5 mentions) Nobiletin, by Yuanye Bio-Technology (5 mentions) Hesperidin, by Yuanye Bio-Technology (5 mentions) Neoeriocitrin, by Yuanye Bio-Technology (4 mentions) Poncirin, by Yuanye Bio-Technology (4 mentions) Limonin, by Yuanye Bio-Technology (3 mentions) Synephrine, by Yuanye Bio-Technology (3 mentions) Neohesperidin, by Yuanye Bio-Technology (3 mentions) Apigenin, by Yuanye Bio-Technology (3 mentions) Hesperetin, by Yuanye Bio-Technology (3 mentions) Rutin, by Yuanye Bio-Technology (3 mentions) Scopoletin, by Yuanye Bio-Technology (2 mentions) Hesperitin, by Yuanye Bio-Technology (2 mentions) Auraptene, by Yuanye Bio-Technology (2 mentions) Methanol, by Sinopharm (2 mentions) Acetonitrile, by Sinopharm (2 mentions) Kaempferol, by Yuanye Bio-Technology (2 mentions)

8 protocols using eriocitrin

1

Satsuma Flavonoid Extraction and Quantification

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The peel and pulp samples of satsumas were thoroughly ground and lyophilized. The 10 ml 80% methanol was added to 0.1 g peel or pulp powder for ultrasonic extraction for 30 min. After centrifugation, the supernatants were filtered with a 0.22 μm nylon membrane for flavonoid analysis.
Flavonoids were determined by HPLC with an Agilent 1,200 series system (Agilent Technologies) and a C18 column (4.6 × 150 mm, 5 μm) according to Cheng's method (Cheng et al., 2017). As shown in Figure S1, the standards (eriocitrin, narirutin, naringin, hesperidin, neohesperidin, didymin, and nobiletin) (Shanghai yuan ye Bio‐Technology Co., Ltd) were applied for qualitative and quantitative analysis of satsuma flavonoids.
Deng W., Wu J., Da Y, & Ma Z. (2020). Effect of temperature treatment on fruit quality and immunoregulation of Satsuma (Citrus unshiu Marc.) during storage. Food Science & Nutrition, 8(10), 5443-5451.
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2

Analysis of Phytochemicals in Herbal Extracts

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Deionized water was used for HPLC-Q-TOF-MS and HPLC-Q-TOF-MS/MS analysis and was purified using a Milli-Q system (Merck Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and formic acid were purchased from Merck (Darmstadt, Germany) and ROE (Newark, New Castle, USA), respectively. Methanol (AR) was purchased from the National Institutes for Food and Drug Control (Beijing, China). The seventeen reference substances (seven flavonol glycosides, three flavonols, two polymethoxyflavonoids, three coumarins, one alkaloid, and one limonin), including synephrine (15), neoeriocitrin (36), poncirin (39), eriocitrin (40), naringin (42), naringenin (43), narirutin (44), neohesperidin (51), hesperidin (56), apigenin (59), hesperitin (61), xanthotoxol (71), scopoletin (76), auraptene (80), limonin (79), nobiletin (85), and tangeretin (87) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).
Yu L., Chen M., Liu J., Huang X., He W., Qing Z, & Zeng J. (2020). Systematic Detection and Identification of Bioactive Ingredients from Citrus aurantium L. var. amara Using HPLC-Q-TOF-MS Combined with a Screening Method. Molecules, 25(2), 357.
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3

Analytical Protocol for Gastrointestinal Dysmotility

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SMD, used for gastrointestinal dysmotility in China (approval number: guo-yao-zhun-zi Z20025044; specification: 10 mL/division), was obtained from Hunan Hansen Pharmaceutical Company, Ltd. (Yi Yang, China). HSA was acquired from Sigma Chemical Co. (St. Louis, MO, USA), standards, including arecaidine, arecoline, norisoboldine, linderane, costunolide, dehydrocostus lactone, synephrine, rutin, limonin, eriocitrin, narirutin, naringin, hesperidin, heohesperidin, poncirin, naringenin, hesperetin, nobiletin, tangeretin, and sorbic acid with a purity of over 98%, were purchased from Yuan-ye Bio-Technology Co., Ltd. (Shanghai, China). The formic acid, acetonitrile, and methanol used for HPLC analysis were chromatographic grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
He Y., Cheng P., Wang W., Yan S., Tang Q., Liu D, & Xie H. (2018). Rapid Investigation and Screening of Bioactive Components in Simo Decoction via LC-Q-TOF-MS and UF-HPLC-MD Methods. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(7), 1792.
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4

Profiling Aurantii fructus Metabolites

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Eleven samples of Aurantii fructus (AF) samples from different habitats were collected and identified by Associate Professor Tang Qi. Reference standards included synephrine, eriocitrin, neoeriocitrin, rutin, narirutin, naringin, hesperidin, neohesperidin, limonin, xanthotol, poncirin, naringenin, hesperetin, nobiletin, tangeretin and auraptene with a purity of over 98% were purchased from Yuan-ye Bio-Technology Co., Ltd. (Shanghai, China). Chromatographic grade formic acid, methanol, ethanol and acetonitrile were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and used for extractive and analytical procedures.
He Y., Li Z., Wang W., Sooranna S.R., Shi Y., Chen Y., Wu C., Zeng J., Tang Q, & Xie H. (2018). Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2189.
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5

Flavonoid Compound Procurement Protocol

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Naringenin, eriodictyol, apigenin, luteolin, liquiritigenin, butin, sakuranetin, kaempferol, quercetin, dihydrokaempferol, dihydroquercetin, eriocitrin, pinocembrin, narirutin, naringin, chrysin, genkwanin, baicalein, scutellarein, norwogonin, wogonin, and neoeriocitrin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Hesperidin, neoHesperidin, sinensetin, isosinensetin, and nobiletin were obtained from Sigma-Aldrich (St Louis, MO, USA). 7-O-methyleriodictyol, isosakuranetin, and 5-HPMF were purchased from BioBioPha Co., Ltd (Kunming, China).
Liu X., Gong Q., Zhao C., Wang D., Ye X., Zheng G., Wang Y., Cao J, & Sun C. (2022). Genome-wide analysis of cytochrome P450 genes in Citrus clementina and characterization of a CYP gene encoding flavonoid 3′-hydroxylase. Horticulture Research, 10(2), uhac283.
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6

Comprehensive Citri Reticulatae Pericarpium Analysis

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Citri Reticulatae Pericarpium was supplied by Scientific Research Institute of Beijing Tongrentang Co., Ltd. Total 21 reference standards, including vicenin-2 (6), vicenin-3 (25), eriocitrin (40), isoquercitroside (45), troxerutin (49), rhoifolin (66), naringin (67), naringenin (68), naringenin-7-O-glucoside (71), hesperidin (78), neohesperidin (82), hesperetin (108), isosinensetin (122), sinensetin (133), quercetagetin-3,5,6,7,3′,4′-hexamethyl ether (144), nobiletin (151), 4´,5,6,7-tetramethoxyflavone (153), tangeretin (168), 5-demethylnobiletin (174), 8-hydroxy-3,5,6,7,3′,4′-hexamethoxyflavone (180), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (181), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The purity of all the reference standards was > 98%. The distilled water was obtained from Watsons, whereas LC–MS grade formic acid was obtained from Fisher-Scientific (Fair Lawn, USA). The LC–MS-grade acetonitrile and methanol were purchased from Merck (Germany).
Wang H.P., Lin Z.Z., Wang H., Yang X, & Niu N. (2024). Comprehensive identifying flavonoids in Citri Reticulatae Pericarpium using a novel strategy based on precursor ions locked and targeted MS/MS analysis. Scientific Reports, 14, 9679.
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7

Enzymatic Inhibition Assay Reagents

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Rutin, eriocitrin, naringin, hesperidin, didymin, poncirin, naringenin, hesperitin, sinensetin, nobiletin, tangeretin, α-glucosidase (from Saccharomyces cerevisiae), acarbose and 5-O-demethylnobiletin were from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Tryptone and yeast extract were from Oxoid Co., Ltd. (Basingstoke, UK). Acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid were from Dr. Ehrenstorfer (Augsburg, Germany). Sodium glycocholate was from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Other reagents and solvents were analytical grade, from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).
Li P., Yao X., Zhou Q., Meng X., Zhou T, & Gu Q. (2022). Citrus Peel Flavonoid Extracts: Health-Beneficial Bioactivities and Regulation of Intestinal Microecology in vitro. Frontiers in Nutrition, 9, 888745.
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8

Acquisition of Bioactive Compounds

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Quercetin, eriodictyol, eriocitrin, neoeriocitrin, kaempferol, luteolin, apigenin, oroxylin A, baicalein 7-O-methyl ether, gallic acid, feruloyl-CoA, and scopoletin were purchased from Shanghai Yuanye Biotechnology Co., LTD (Shanghai, China). Baicalein, isorhamnetin, and SAM were purchased from Aladdin (Shanghai, China). Chrysoeriol and homoeriodictyol were purchased from BioBioPha (Kunming, China). 7,8-dihydroxyflavone and 3′,4′-dihydroxyflavone were purchased from TCI (Shanghai, China). 8-Hydroxy-7-methoxyflavone was purchased from Biosynth (St. Gallen, Switzerland) and caffeic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA). Esculetin was purchased from SinoStandards (Chengdu, China) and caffeoyl-CoA was purchased from ZZStandard (Shanghai). The chromatographic grade acetonitrile and methanol for HPLC were purchased from Sigma-Aldrich.
Liao Z., Liu X., Zheng J., Zhao C., Wang D., Xu Y, & Sun C. (2023). A multifunctional true caffeoyl coenzyme A O-methyltransferase enzyme participates in the biosynthesis of polymethoxylated flavones in citrus. Plant physiology, 192(3).
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