Aia 2000 la

The OGTTs were performed after 10‐hour fasts with 0‐, 30‐, 60‐ and 120‐minute samplings to establish plasma glucose and insulin levels, and at the preload time, serum high‐density lipoprotein cholesterol (HDL‐C), low‐density lipoprotein cholesterol (LDL‐C), triglyceride (TG), haemoglobin A1c (HbA1c) and glycoalbumin (GA) were measured. Excluded from this study were subjects who were over 30 years of age.
We classified 575 subjects into the NGT group, 19 into the IGT group, and 1 into the IFG group. We removed the subjects with IGT and IFG from further analyses in this analysis. We found no subjects with apparent T2DM.
We measured height and weight and calculated BMIs (weight [kg]/height [m²]). We used enzymatic methods to measure serum HDL‐C, LDL‐C, TG and GA concentrations, with an automatic analyser (LABOSPECT 008; Hitachi). Serum insulin concentrations were measured by chemiluminescence immunoassay using an automatic analyser (AIA‐2000 LA; Tosoh). Plasma glucose concentrations were measured using a hexokinase method, and HbA1c levels were measured by high‐performance liquid chromatography, using automatic analysers (ADAMS Glucose GA‐1170 and ADAMS A1c HA8180, respectively; Arkray).

Hematocrit, white blood cell (WBC) count, and platelet count were measured using an automated hematology analyzer (Sysmex XE-5000; Sysmex Corporation, Kobe, Japan). Fibrinogen concentration was measured by the Clauss method using an automated coagulation analyzer (Sysmex CS-5100; Sysmex Corporation). Serum TC, TG, HDL-C, and LDL-C levels were measured by enzymatic methods, and C-reactive protein (CRP) levels were measured by latex immunoassay, using an automatic analyzer (LABOSPECT008; Hitachi High-Technologies Corporation, Tokyo, Japan). Serum insulin levels were measured by chemiluminescence immunoassay using an automatic analyzer (AIA-2000 LA; Tosoh Corporation, Tokyo, Japan). Plasma glucose levels were measured by the hexokinase method, and hemoglobin A1c levels were measured by high-performance liquid chromatography, using automatic analyzers (ADAMS Glucose GA-1170 and ADAMS A1c HA-8180; ARKRAY, Inc., Kyoto, Japan). Whole blood viscosity levels were measured at 60 revolutions/min using a micro cone-plate viscometer (LVDV-II + Pro CP; Brookfield Engineering Laboratories, Middleboro, MA, USA) [36 (link)]. The inter- and intra-assay coefficients of variation for BPT were 7% and 5% , respectively.