Ficoll density separation

Manufactured by Merck Group

Sourced in Switzerland

Ficoll density separation is a laboratory technique used for the isolation and purification of cells, organelles, and other biological particles based on their density. It involves the use of a Ficoll, a synthetic polymer, to create a density gradient that allows the separation of different components in a sample.

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Lab products found in correlation

Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by PAN Biotech (3 mentions) α mem, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ficoll (251 citations) Ficoll density gradient separation (6 citations) Ficoll density gradient separation method (3 citations) Ficoll separation (2 citations)

3 protocols using ficoll density separation

Isolation and characterization of hBMSCs

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Bone marrow was obtained with full ethical approval (KEK-ZH-NR: 2010–0444/0) and the written consent from patients undergoing routine operations due to bone fracture. MSCs were isolated from four different marrow aspirates (one female 25-year old and three males 19, 50 and 66 years old) using Ficoll density separation (Sigma-Aldrich, Buchs, Switzerland).
Mononuclear cells were collected from the interphase seeded at a density of 50,000 cells/cm² in alpha-minimum essential medium (αMEM) (Gibco, Carlsbad, CA, USA), 10% MSC tested Fetal Bovine serum (FBS) (Pan Biotech, Aidenbach, Germany), 5 ng/mL basic fibroblast growth factor (bFGF) (Peprotech, Rocky Hill, CN, USA) and 1% penicillin/streptomycin (Gibco). Media was refreshed after 96 h. The cells were passaged when 70% confluent and seeded at a cell density of 3000 cells/cm². The chondrogenic potential of each donor was confirmed using standard techniques. hBMSCs isolated from each donor were used separately in four independent experiments.

Monaco G., Ladner Y.D., El Haj A.J., Forsyth N.R., Alini M, & Stoddart M.J. (2021). Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro. Cells, 10(8), 2165.

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Isolation and Characterization of hBMSCs

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Bone marrow was obtained with full ethical approval (KEK-ZH-NR: 2010–0444/0) and the written consent from patients undergoing routine operations due to bone fracture.
The MSCs were isolated from three different marrow aspirates (two female 1939 and 1992, one male 1958) using Ficoll density separation (Sigma-Aldrich, Buchs, Switzerland) (Table 1).
Mononuclear cells were collected from the interphase and the adherent cell fraction was seeded at a density of 50,000 cells/cm² and left to attach for 96 hrs in alpha minimum essential medium (αMEM) (Gibco, Carlsbad, CA, United States), 10% MSC tested fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany), 5 ng/ml basic fibroblast growth factor (bFGF) (Peprotech, Rocky Hill, CN, United States) and 1% penicillin/streptomycin (Gibco). When the majority of colonies were confluent, the cells were passaged and seeded into fresh flasks at a cell density of 3,000 cells/cm². The chondrogenic potential of each donor was confirmed using standard techniques.
The hBMSCs isolated from each donor were used separately in three independent experiments.

Monaco G., El Haj A.J., Alini M, & Stoddart M.J. (2020). Sodium Hyaluronate Supplemented Culture Media as a New hMSC Chondrogenic Differentiation Media-Model for in vitro/ex vivo Screening of Potential Cartilage Repair Therapies. Frontiers in Bioengineering and Biotechnology, 8, 243.

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Isolation of Mesenchymal Stem Cells from Bone Marrow

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Mesenchymal stem cells were isolated from bone marrow aspirates collected during routine operations with full‐ethical approval (KEK‐ZH‐NR: 2010–0444/0). The MSCs were from four different marrow aspirates from vertebral bodies (two female aged 18 years and 49 years, two male aged 22 years and 76 years) and one from tibial plateau (males aged 48 years). Mononuclear cells were isolated from whole bone marrow of each donor using Ficoll density separation (Sigma‐Aldrich, Buchs, Switzerland). Isolated mononuclear cells were then seeded at a density of 50 000 cells/cm² and left to attach for 96 h in alpha minimum essential medium (αMEM) (Gibco, Carlsbad, CA, USA), 10% MSC tested fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany), 5 ng/ml basic fibroblast growth factor (bFGF) (Peprotech, Rocky Hill, CN, USA) and 1% penicillin/streptomycin (Gibco). MSCs isolated from vertebral bodies were used for the main body of experiments, while MSCs isolated from the tibial plateau were used for cell tracking experiments.

Gardner O.F., Musumeci G., Neumann A.J., Eglin D., Archer C.W., Alini M, & Stoddart M.J. (2016). Asymmetrical seeding of MSCs into fibrin–poly(ester‐urethane) scaffolds and its effect on mechanically induced chondrogenesis. Journal of Tissue Engineering and Regenerative Medicine, 11(10), 2912-2921.

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