5977a inert mass selective detector

Approximately, 0.1 g of seeds at different developmental stages were ground frizzed in liquid nitrogen and extracted with 1 mL hexane using an ultrasonic cleaner for 30 min, and then incubated at 40°C for 1 h. The samples were then centrifuged at 10,000 rpm for 15 min and the resulting supernatants were pipetted into novel 2 mL tubes. One milliliter of hexane extract was pipetted into 1 mL vial for GC- mass spectrometry (MS) analysis. The extraction was analyzed using Agilent 7890B Gas Chromatograph with 5977A inert Mass Selective Detector (Agilent, United States). Helium was used as the carrier gas (1 mL/min) and then separated on the CycloSil-B column (30 m × 0.25 mm id, 0.25 mm film thickness). The GC oven temperature was programmed at an initial temperature of 35°C for 2 min, and then at 5°C/min from 35to 200°C, increase to 230°C at 10°C/min, and hold at 230°C for 2 min. The temperature was then kept at 240°C for 5 min. NIST14/Wiley275 Mass Spectral Library was used for metabolite identification. The terpene compounds were identified by the mass spectral library. The predominant terpene acetates and their precursor terpene alcohols in this research were further identified using their authentic standards. There were three biological replicates and three technical replicates for each organ.

Approximately, 0.25 g of leaf samples extracted with 50 mL Ethyl acetate using an ultrasonic cleaner for 20 min, repeat two times, and then concentrated by rotary evaporation. Concentrate dissolved in hexane to a volume of 5 mL. The extraction was analyzed using Agilent 7890B Gas Chromatograph with 5977A inert Mass Selective Detector (Agilent, United States). The injection volume is 1 μl of continuous filtrate, and helium is carrier gas. Agilent HP-5MS column (30 m × 250 mm × 0.25 mm film thickness) as a column for separation. GC oven temperature was programmed at an initial temperature of 50 °C for 2 min with an increase of 20 °C/min to 130 °C, and raised to 150 °C at a rate of 2 °C / min for 5 min. And temperature was then raised to 230 °C at a rate of 20 °C / min. Quadrupole and ion source temperatures are set to 150 °C and 230 °C. NIST14/Wiley275Mass Spectral Library was used for metabolite identification. This study used the external standard method to quantify the patchouli alcohol; there were three biological replicates and two technical replicates for each tissue.

Mature leaves (0.2 g) were ground frizzed in liquid nitrogen, with 1.5 mL hexane ultrasonic extraction for 25 min, and then under the 56 °C water bath for 1 h. The sample was centrifuged at 8000 rpm for 10 min, and the supernatants were transferred into new vials for GC–MS analysis. GC–MS was performed using an Agilent 7890B Gas Chromatograph with 5977A inert Mass Selective Detector (Agilent, United States). The gas chromatograph was equipped with an HP-5MS capillary column (30 m × 250 mm × 0.25 mm film thickness). The oven temperature was programmed from 35 °C (5 min hold) to 300 °C at a rate of 12 °C/min. NIST14/Wiley275 Mass Spectral Library was used for metabolite identification. The terpene compounds were identified by the mass spectral library. The content of volatile terpenes was quantified by using cyclohexanone as an external standard.