Growth chamber

Manufactured by Sanyo

Sourced in Japan

The Growth Chamber is a controlled environment device designed for the cultivation and study of plants, microorganisms, and other biological specimens. It maintains consistent temperature, humidity, and lighting conditions to support optimal growth and development.

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Lab products found in correlation

Hygromycin b, by Roche (1 mentions) Murashige and skoog, by Duchefa Biochemie (1 mentions) Hoagland s nutrient solution, by Merck Group (1 mentions)

Spelling variants of this product

MLR-350-H growth chamber (4 citations) Plant growth chamber (4 citations) MLR-350 growth chamber (2 citations)

15 protocols using growth chamber

Genetic Engineering of Arabidopsis and Tobacco Plants

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All wild-type and transgenic A. thaliana plants used in this study are in Columbia-0 (Col-0) background. They were germinated and grown in a growth chamber (Sanyo) at 20 °C on a 16 h light, 8 h dark cycle.
All Nicotiana tabacum plants used in this study are wild-type cv. Petit Havana SR1. Plants were germinated and grown in a controlled environment chamber at 20 °C on a 16 h light, 8 h dark cycle. Following infiltration, they were placed in a growth chamber (Sanyo) at 21 °C on a 14 h light, 10 h dark cycle and 70% humidity.
Generation of A. thaliana plants stably overexpressing genes of interest was achieved by floral dipping of Col-0 plants^{58 (link)}. Transgenic seedlings were identified based on sowing seeds on 15 µg/mL phosphinotricin and screening established seedlings for GFP fluorescence. A. thaliana plants stably overexpressing GFP-MRF7 or GFP-ΔMRF7 were crossed with lines overexpressing the Golgi marker ST-mRFP (Chris Hawes group, Oxford Brookes University).

Perico C., Gao H., Heesom K.J., Botchway S.W, & Sparkes I.A. (2021). Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor. Communications Biology, 4, 1182.

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Poplar Transformation and Gene Expression Analysis

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The sampled plantlets of the elite clone (P. deltoides × P. euramericana cv. “Nanlin895”) in this study were cultivated on Murashige and Skoog (MS) medium (pH 5.8) containing 3% (w/v) sucrose and 0.2% (w/v) Gelrite in a growth chamber (SANYO, Tokyo, Japan) at 25/18 °C (day/night), daily photoperiod of 16/8 h (light/dark, illumination of 50 µmol·m⁻²s⁻¹), and relative humidity of 60–80%. Transformation acceptor plantlets (P. davidiana × P. bolleanan) were maintained, transformed, and regenerated as previously described.
To determine the expression of target gene in “Nanlin895” poplar and transgenic plants, various organs (roots, stems, and leaves) from 40-day-old plants were harvested and stored at −80 °C until RNA extraction. For the salt-stress treatment, 40-day-old plants were transferred from initial solid MS medium (without NaCl) to liquid MS media containing either 100 mM and 300 mM NaCl for different times: 0, 2, 6, 12, 24, and 72 h. Two treatments of plants were used for growing, as previously described. After treatment, various tissues were harvested from three clonal plants of each treatment at each time point, frozen immediately in liquid nitrogen, and then stored at –80 °C for RNA isolation.

Wang J., Sun Z., Chen C, & Xu M. (2022). The MKK2a Gene Involved in the MAPK Signaling Cascades Enhances Populus Salt Tolerance. International Journal of Molecular Sciences, 23(17), 10185.

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Soybean Root Growth Dynamics

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Seeds of soybean (cv. Enrei) were sterilized with 2% sodium hypochlorite solution and washed in clean water. The sterilized seeds were sown 4 cm inside 450 mL of quartz sand in seedling cases (145 x 55 x 95 mm³) wetted with 150 mL water and grown at 25°C and 70% humidity in a growth chamber (Sanyo, Tokyo, Japan) under fluorescent light (160 μmol m^-2 s^-1, 16 h light period/day). Eight seeds were grown in each pot per treatment. Two-day-old soybeans were flooded until day 6. The roots were washed with tap water to remove sand particles and cut with scissor. The root samples were collected at days 2, 3, 4, 5 & 6 from un-treated control [labeled as 2(0), 3(0), 4(0), 5(0), 6(0)] and treated [labeled as 3(1), 4(2), 5(3), 6(4)] plants (Fig 1). Three independent biological replications were performed for each type of experiment.

Khan M.N., Ahmed I., Ud Din I., Noureldeen A., Darwish H, & Khan M. (2022). Proteomic insight into soybean response to flooding stress reveals changes in energy metabolism and cell wall modifications. PLoS ONE, 17(5), e0264453.

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Maize Growth Conditions for Experiments

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Maize (Zea mays var. saccharata, Canberra 90EX) seeds were obtained from Takii Seed Corporation (Kyoto, Japan) and sown in 128-hole trays (30 × 60 cm; hole size: 3 cm diameter and 4.5 cm depth; Home Center Musashi, Niigata, Japan) filled with potting soil to a depth of 3.5 cm and covered with 1 cm of porous silicate (Inenica Krion Corporation, Aichi, Japan). The trays were kept in the laboratory under a 16/8 h photoperiod at about 25°C for two days to confirm germination. Then, the trays were placed in a plastic tunnel (100 cm long, 60 cm wide, and 60 cm high; Figure 1A) installed in the field at the experimental station of Niigata University in Niigata City, Japan, in June 2022 for field experiments, and in a climate-controlled chamber (Growth chamber, SANYO, Osaka, Japan) under a 16/8 h photoperiod at 25 ± 3°C, for laboratory experiments.

Sakurai Y., Ishizaki S., Izumi S., Yoshida T., Shiojiri K, & Takabayashi J. (2023). The exposure of field-grown maize seedlings to weed volatiles affects their growth and seed quality. Frontiers in Plant Science, 14, 1141338.

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Generation and Validation of Transgenic Arabidopsis Plants

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The ClmiR86-pMDC83 construct was introduced into Agrobacterium tumefaciens strain GV3101. Wildtype Arabidopsis was transformed using the floral dip method [73 (link)]. To screen for transgenic plants, seeds harvested from transformed Arabidopsis plants were germinated on MS medium containing 50 μg·mL⁻¹ hygromycin B (Roche, http://www.roche.com; accessed on 10 September 2021 ), vernalized at 4 °C for 3 days, and incubated in a growth chamber (Sanyo) for 10 days. The 10-day-old hygromycin B-resistant plants were then transferred to the Jiffy seedling culture substrate. PCR and quantitative real-time PCR (qRT-PCR) of ClmiR86 in candidate transgenic Arabidopsis plants were used to confirm the success of transformation (Figures S1 and S2A). WT and T3 plants were used in the present study. All primers used for the confirmation of Arabidopsis transformation are listed in Table S3.

Wu W., Zhao H., Deng Q., Yang H., Guan X., Qi R., Shi P., Yang J., Zhang M, & Hu Z. (2021). The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon. Plants, 10(10), 2133.

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Germination of Arabidopsis Mutant Seeds

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Seeds of Col-0, En-2, dwf1, det2, 35S:DWF1, det2 35S:DWF1, bes1-D, and bzr1-1D were each surface-sterilized in ethanol–water (70:30, v/v), rinsed in distilled water (DW), cold-treated at 4 °C for 2 d and plated on 0.5× Murashige and Skoog (Duchefa, Haarlem, the Netherlands) medium containing 1% sucrose and 0.7% agar. Plates were kept in the light (120 μmol m⁻² s⁻¹) at 22 °C for 16 h and in the dark for 8 h in a growth chamber (Sanyo, Osaka, Japan).

Youn J.H., Kim T.W., Joo S.H., Son S.H., Roh J., Kim S., Kim T.W, & Kim S.K. (2018). Function and molecular regulation of DWARF1 as a C-24 reductase in brassinosteroid biosynthesis in Arabidopsis. Journal of Experimental Botany, 69(8), 1873-1886.

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Tomato Seed Germination Under Copper Stress

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Surface sterilized Solanum lycopersicum L. commercial cv. H2274 (MayAgro Seed Corporation, Turkey) seeds were used in the experiments. Experimental setup is presented in Fig. 1. Twenty germinated seeds for each replicate were transferred to sterile 1/2 strength Hoagland's nutrient solution (Sigma, USA). All the groups contained at least 4 replicates. Treatment groups contained 100 μg mL -1 jute or nettle extracts in the Hoagland's solution (treatment 1), or seeds were pre-soaked into 100 μg mL -1 jute or nettle extract solutions (treatment 2) for 24 h prior to germination.
Seedlings were incubated at 23-25 °C, at dark, by shaking. Then, the seeds were left to germinate for 2 days at dark in layers of wet course filter papers. Extracts were not applied to untreated groups. Treated and untreated seeds were further separated into two groups, i.e. incubation with or without copper. In our previous study, we determined the effective copper sulphate (CuSO 4 •5H 2 O) concentration (EC 50 ) for tomato as 30 ppm [13] . EC 50 (30 ppm) were applied to copper stress groups. All the experimental groups were incubated at 23-25 °C, with 50 ± 5% relative humidity, and a photoperiod of 16/8 h (day/night) in growth chamber (Sanyo, Japan) for 7 days. After incubation period, roots were sampled for further analysis.

İşeri Ö.D., Körpe D.A., Sahin F.I, & Haberal M. (2018). Corchorus olitorius and Urtica pilulifera extracts alleviate copper induced oxidative damage and genotoxicity in tomato. Acta biologica Hungarica, 69(3).

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Soybean Seedling Growth Protocol

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Seeds of soybean (cv. Enrei) were sterilized with 2% sodium hypochlorite solution and washed in clean water. The sterilized seeds were sown 4 cm inside quartz sand in seedling cases (145 x 55 x 95 mm 3 ) wetted with 150 mL water and grown at 25°C in a growth chamber (Sanyo, Tokyo, Japan) under fluorescent light (160 μmol m -2 s -1 , 16 h light period/day). Eight seeds were grown in each pot per treatment. Two-day-old soybeans were flooded until day 6. The root samples were collected at days 2, 3, 4, 5 & 6 from un-treated control [labeled as 2(0), 3(0), 4(0), 5(0), 6(0)] and treated [labeled as 3(1), 4(2), 5(3), 6(4)] plants (Fig 1).

Khan M.N., Ahmad I., Israr-ud-Din, Ali E., Noureldeen A., Darwish H., Khan M.I., Tayyab M., & Khan M. (2021). Proteomic insight into soybean response to flooding stress reveals changes in basic energy metabolism and cell wall modifications.

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Seed Emergence and Growth Assessment Protocol

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Treated and untreated seeds with four replications were sown in plastic trays (25 seeds in each replication) containing moist sand, and were placed in a growth chamber (SANYO, Osaka, Japan) at 25 °C with continuous fluorescent light during the course of investigation. Emergence was recorded daily on the basis of appearance of cotyledons. Seedlings were harvested after two weeks and washed with deionized water and subsequently, seedlings root-shoot lengths, fresh and dry mass (after oven drying the samples at 65 °C for three days) were recorded

Mean Emergence Time (MET) = \frac{Σ Dn}{Σ n}

where n is the number of seeds which emerged on day D, and D is the number of days counted from the beginning of emergence.

Emergence Index (EI) = \frac{No . of emerged seeds}{Days of first count} + \frac{No . of emerged seeds}{Days of final count}

Afzal I., Saleem S., Skalicky M., Javed T., Bakhtavar M.A., ul Haq Z., Kamran M., Shahid M., Sohail Saddiq M., Afzal A., Shafqat N., Dessoky E.S., Gupta A., Korczyk-Szabo J., Brestic M, & E. L. Sabagh A. (2021). Magnetic Field Treatments Improves Sunflower Yield by Inducing Physiological and Biochemical Modulations in Seeds. Molecules, 26(7), 2022.

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Measuring Root Elongation in Rapeseed

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To select the strains that were applied in experiment 1, a rapeseed test was previously performed (Figure 1). This species is sensitive to IAA changes, which are reflected in the elongation of roots [41 (link)]. Therefore, after inoculating 40 seeds of B. napus for each treatment, they were distributed into four sterile growth bags (DIK-710A, Daiki^®, Kounosu, Japan) containing 12 mL Hewitt solution 0.5× [42 ]. The bags were placed in a growth chamber (Sanyo Electric^®, Osaka, Japan) with a 12 h light and 12 h dark cycle, illuminated by a light source (λ = 400–700 nm) with a flux density of 250 μmol m⁻² s⁻¹, and 80% relative humidity for seven days. After this period, the length of the radicle was measured.

Barcia-Piedras J.M., Pérez-Romero J.A., Mateos-Naranjo E., Parra R., Rodríguez-Llorente I.D., Camacho M, & Redondo-Gómez S. (2023). Stimulation of PGP Bacteria on the Development of Seeds, Plants and Cuttings of the Obligate Halophyte Arthrocaulon (Arthrocnemum) macrostachyum (Moric.) Piirainen & G. Kadereit. Plants, 12(7), 1436.

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