Cto 10avp column oven

Manufactured by Shimadzu

Sourced in Japan

The CTO-10AVP is a column oven manufactured by Shimadzu. It is designed to precisely control the temperature of chromatographic columns during analysis, maintaining a stable environment for consistent and reliable results.

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Column oven (9 citations) CTO-10Avp (4 citations)

5 protocols using cto 10avp column oven

Phenolic Compounds Analysis by HPLC

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Phenolic compounds were analyzed by high-performance liquid chromatography (HPLC) equipped with two UV/VIS detectors (SPD-10A UV/VIS Detector; Shimadzu SPD-20AV UV-Vis Detector, Shimadzu, Kyoto, Japan), a column oven (CTO 10AVP Column Oven, Shimadzu) and a reverse-phase C18 column (Prevail 5 µm organic acid, 4.6 mm × 250 mm, Hichrom, Leicestershire, UK). The mobile phase solution consisted of 2.5 mM KH₂PO₄ and acetonitrile (60:40 v/v). The flow rate applied was 1.0 mL/min. Then, 20 μL of the sample was injected at 40 °C with a run time of 20 min. Two UV/VIS detectors were used with detection wavelengths set to either 305 nm or 280 nm. Phenols in each sample were analyzed in triplicate.
Phenols of each compound were identified by comparing the retention time of the external standard with that of the internal standard. Phenolic standard solutions were prepared by dissolving in methanol or acetonitrile. A calibration curve was created by analyzing the standard solutions of each compound diluted to several concentrations. Quantification was performed using the peak area and the calibration curve of each compound.

Han S., Yang J., Choi K., Kim J., Adhikari K, & Lee J. (2022). Chemical Analysis of Commercial White Wines and Its Relationship with Consumer Acceptability. Foods, 11(4), 603.

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HPLC-MS Simultaneous Ginsenoside Quantification

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A HPLC system consisted of a LC-10AD pump, a DGU-14 AM degasser, a Shimadzu 10ATvp auto-sampler and a CTO-10Avp column oven (Shimadzu Corporation, Kyoto, Japan) was employed to achieve simultaneous detection of all analytes. Separation was performed on a Shimadzu 2010 liquid chromatograph–mass spectrometer (Shimadzu Corporation) with a LUNA C18 column (150 mm × 2 mm, 5 μm, Phenomenex®, Los Angeles, CA, USA). The mobile phase was consisted of 0.1 mmol/L ammonium chloride solution (A) and acetonitrile (B) and the flow rate was set at 0.2 mL/min. A gradient elution program was used as follows: 0.04 → 1.5 min, B% 25 → 25; 1.5 → 12.0 min, B% 25 → 45; 12.0 → 19.0 min, B% 45 → 90; 19.0 → 22.0 min, B% 90 → 90; 22.0 → 23.0 min, B% 90 → 25; 23.0 → 29.0 min, B% 25 → 25. Quantification was performed using SIM mode with [M+Cl]⁻ peak which was modified from our previous method²¹ (link): m/z 1143.3 for Rb1; m/z 1113.3 for Rb2; m/z 1113.3 for Rc; m/z 981.4 for Rd; m/z 835.4 for Rg1; m/z 981.4 for Re; m/z 819.3 for Rg3; m/z 955.3 for Ro; m/z 815.4 for digoxin. The concentration range in plasma was 5–2000 ng/mL for Rb1 and Rb2, 5–1000 ng/mL for Rc, Rd, Rg1, Re and Ro, and 5–500 ng/mL for Rg3. The calibration curve of all ginsenosides detected in tumor showed good linearity over the concentration range of 5–500 ng/mL.

Zhong C., Jiang C., Ni S., Wang Q., Cheng L., Wang H., Zhang Q., Liu W., Zhang J., Liu J., Wang M., Jin M., Shen P., Yao X., Wang G, & Zhou F. (2019). Identification of bioactive anti-angiogenic components targeting tumor endothelial cells in Shenmai injection using multidimensional pharmacokinetics. Acta Pharmaceutica Sinica. B, 10(9), 1694-1708.

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HPLC Analysis of Phenolic Compounds

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HPLC analysis was carried out using a Shimadzu binary gradient unit LC-10AD VP (Shimadzu Instruments, Kyoto, Japan) system, equipped with a SCL10AVP system controller, SPP-M20A Prominence Diode Array Detector, LC-10ADSP binary pumps, CTO-10AVP column oven and SIL-10AF autosampler [55 (link)]. Separation was performed on a Teknokroma Mediterranean Sea 18 15 × 0.46 cm, i.d. 5 µm column, maintaining a flow rate of 1 mL/min. The gradient consisted of 2.5 pH (adjusted with orthophosphoric acid) water (solvent A) and acetonitrile (solvent B). Starting gradient was 5% solvent B, changed to 9% B in 3 min (min 12 of separation); min 20, 13% B; min 30, 33% B; min 42 to 60 liniar gradient to 43% B; min 65, 90% B; min 70, 100% B, than decreasing to 5% B until min 78. Column temperature was maintained to 24 °C (CTO-10AVP column oven, Shimadzu Corporation, Kyoto, Japan), and the separation was monitored at 220–400 nm [56 ]. Commercial standards of phenolic acids: gallic, p-OH-benzoic, chlorogenic, caffeic, siringic, ferulic and rosmarinic acid, flavonoids (catechin, quercetin, rutin, hyperosid, quercitrin, miricitrin, isoquercitrin, luteolin, apigenin and kaempherol) were used as reference compounds.

Clapa D., Borsai O., Hârța M., Bonta V., Szabo K., Coman V, & Bobiș O. (2020). Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L.. Plants, 9(5), 656.

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Phenolic Profiling of Olive Pomace Extract

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Phenolic profiles of OPE were determined by HPLC coupled to a diode array (HPLC–DAD). Standard calibration curves were prepared by using gallic, protocatechuic acid, catechin, p-hydroxybenzoic, syringic, elagic, m-coumaric, o-coumaric, myricetin, quercetin, kaempferol, hydroxytyrosol, tyrosol, and luteolin. The HPLC analysis was performed using a modified method [5 (link)]. The samples and stock solutions were filtered through a 0.45 µm membrane filter and analyzed in a Shimadzu HPLC system (LC-10AD vp pump, SPDM10A vp DAD detector, SIL-10AD vp autosampler, CTO-10AVP column oven, DGU-14A degasser, and SCL-10A system controller; Shimadzu Corp., Kyoto, Japan). Separations were performed at 30 °C on Agilent Eclipse XDB-C18 reversed-phase column (250 mm × 4.6 mm length, 5 μm particle size). The mobile phase contained solvent A (3% (v/v) acetic acid) and solvent B (methanol). A gradient elution was carried out as shown: 28% B (0–20 min), 28–30% B (21–50 min), 31–50% B (51–70 min), and 50–100% B (70–81 min) and at 90 min was returned to initial conditions. The flow rate was 0.8 mL/min. Chromatograms were recorded at 278 nm. Identification and quantitative analysis were made based on the retention times and external standard curves. The amounts of polyphenols were stated in μg/g of dried olive pomace extract.

Akcicek A., Bozkurt F., Akgül C, & Karasu S. (2021). Encapsulation of Olive Pomace Extract in Rocket Seed Gum and Chia Seed Gum Nanoparticles: Characterization, Antioxidant Activity and Oxidative Stability. Foods, 10(8), 1735.

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Molecular Weight Assessment of HA–TA Conjugate

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The molecular weight of the HA–TA conjugate was assessed using SEC-MALLS (size exclusion chromatography/multiangle laser light scattering). Samples were dissolved overnight in a mobile phase to produce solutions with a concentration of 2 mg cm⁻³. The chromatographic system consisted of an LC-10ADVP Shimadzu HPLC pump, SIL-10AF autosampler, CTO-10AVP column oven, SCL-10AVP system controller, DGU-14A degasser, RID-10A refractive index detector, SPD-10AVVP UV-VIS detector (all from Shimadzu), and a miniDAWN TREOS light scattering photometer (Wyatt Technology Corporation). The injection volume was 100 μl. Each sample was filtered through a 0.22 μm MS Nylon Syringe Filter. The mobile phase consisted of aqueous 50 mM sodium phosphate and 0.02% sodium azide at a flow rate of 0.8 ml min⁻¹. Data acquisition and molecular weight calculations were performed using the ASTRA software (version 5.3.4, Wyatt Technology Corporation, USA). A specific refractive index increment of 0.155 ml g⁻¹ was used for HA.

Bystroňová J., Ščigalková I., Wolfová L., Pravda M., Vrana N.E, & Velebný V. (2018). Creating a 3D microenvironment for monocyte cultivation: ECM-mimicking hydrogels based on gelatine and hyaluronic acid derivatives. RSC Advances, 8(14), 7606-7614.

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