Fastgene blue green led flashlight

We used a fluorescent and nonpathogenic strain E. coli GFP ATCC^® 25922GFP™ (American Type Culture Collection, Manassas, VA, USA). It harbors a multicopy vector encoding the green fluorescent protein GFPmut3. The GFPmut3 fluorophore has an excitation maximum at 501 nm and an emission maximum at 511 nm, compatible with usual fluorescence detection technologies. The plasmid-borne bla gene confers the resistance to 100 µg/mL of ampicillin. This E. coli strain has been grown in a Luria-Bertrani (LB) broth overnight at 37 °C with gentle shaking to reach the growth plateau (~109 CFU/mL). Then, an aliquot of the culture of 6 mL with a concentration of 2.4 × 10⁶ CFU/mL was inoculated in rhizotrons via a sterile pipette for water inoculation and via sterile syringe connected to a tube to reach a 5-cm depth in sediments to simulate contaminations from the surface water and groundwater, respectively. To make the strain visible, it was excited at each measure by lightening the rhizotrons with a FastGene^® Blue/Green LED Flashlight (Nippon Genetics Europe GmbH, Dueren, Germany) emitting at 480–530 nm.