Mouse testosterone elisa kit

After 8 weeks of GABA treatment, blood samples were obtained through cardiac puncture and centrifuged at 3000× g for 20 min at 4 °C, and serum samples were stored at −80 °C until subsequent analysis. Serum insulin concentrations were measured using a Mouse Metabolic Hormone Magnetic Bead Panel (Merck Millipore, Burlington, MA, USA). Serum concentrations of TG, total cholesterol, low-density lipoprotein (LDL)-cholesterol, and high-density lipoprotein (HDL)-cholesterol were determined using colorimetric assay kits (Roche). Serum concentrations of TNF-α and IL-1β were measured using a Mouse High-sensitivity T Cell Magnetic Bead Panel (Merck Millipore, Burlington, MA, USA). Serum testosterone concentrations were measured using a Mouse Testosterone ELISA Kit (Abcam, Cambridge, UK).