Idk calprotectin

Serum C-reactive protein (CRP) (mg/L) and albumin (g/dL) levels were determined by immunoturbidimetry (Roche, Hitachinaka, Japan). The complete blood count (CBC) was measured using an automated hematology analyzer (Sysmex, Kobe, Japan). The erythrocyte sedimentation rate (ESR) was evaluated according to the Westergren method (mm/h).
fCal concentration was determined by an enzyme-linked immunosorbent assay ELISA kit (IDK Calprotectin, Immundiagnostik, Bensheim, Germany) in accordance with the manufacturer’s instructions. The assay utilizes the two-site sandwich technique with two selected monoclonal antibodies that bind to human calprotectin. The concentration of calprotectin in each sample was determined directly from the standard curve. The upper normal limit was determined as 65 µg of calprotectin per 1 gram of feces.

The biochemical analyses of the blood were performed in the local laboratory. ALT and GGT activities were determined by kinetic method (Cobas 6000-c501, Roche Diagnostics, Mannheim, Germany) and a serum ALT level >37 U/L and GGT values >40 IU/L were considered abnormal. Direct bilirubin was estimated using the Diazo Special method (Cobas 6000-c501, Roche Diagnostics, Mannheim, Germany) and its level >1 mg/dL was considered elevated. Selected markers of inflammation were determined in serum and stool based on the following methods. Faecal calprotectin (FC) concentration was determined by ELISA kit (IDK Calprotectin, Immundiagnostik, Bensheim, Germany). Erythrocyte sedimentation rate (ESR) was evaluated according to the Westergren method (Alifax Roler 20). Serum C-reactive protein (CRP) and albumin levels were determined by immunoturbodimetry (Cobas 6000-c501, Roche Diagnostics Mannheim, Germany). The complete blood count was measured using a Hematology Analyzer (Beckman Coulter). Ferritin was estimated by luminescent electrochemistry (Cobas e411, Roche Diagnostics Mannheim, Germany). Iron (Fe) was evaluated using colorimetric method (Cobas 6000-c501, Roche Diagnostics Mannheim, Germany). The cut-off values of the parameters were based on the local laboratory reference ranges.