Fucoidan from F. vesiculosus was purchased from Sigma-Aldrich, USA (≥95%, CAS:9072-19-9), its monosaccharide composition has been detected (fucose:138.7 ± 5.5; rhamnose: 2.0 ± 0.6; galactose: 27.9 ± 1.4; glucose: 2.5 ± 1.8; xylose: 12.8 ± 1, 6; mannose: 0.2 ± 0.4: glucuronic acid: 18.5 ± 1.9) [25 (link),26 (link)]; Caco-2 cell was purchased from Kunming Institute of Zoology, Chinese Academy of Sciences Kunming Cell Bank; Trypsin was purchased from Invitrogen, US; Penicillin and Streptomycin was purchased from Invitrogen, USA; Chromatographic column (G4000PWXL) was purchased from Agilent, USA; Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Biotechnology, China; DMSO was purchased from Sigma-Aldrich, USA; puromycin was purchased from Aladdin, China; fluorescent yellow was purchased from Aladdin, China; chlorpromazine hydrochloride was purchased from Aladdin, China; Dynasore was purchased from Aladdin, China; Ammonium chloride was purchased from Shanghai Sinopharm Group, China; FITC labeled human transferrin (Chromatographic grade) was purchased from Jackson, USA; Fluorescein isothiocyanate (FITC) was purchased from Aladdin, China; Sodium cyanoborohydride was purchased from Aladdin, China; Fucose was purchased from L-Fucose, Aladdin, China; Transwell Chamber (0.4 μm) was purchased from Millipore, USA.
Methylthiazolyldiphenyl tetrazolium bromide mtt
Manufactured by Beyotime
Sourced in China
Methylthiazolyldiphenyl-tetrazolium bromide (MTT) is a lab equipment product used in colorimetric assays to measure cell metabolic activity. It is a yellow tetrazolium salt that can be reduced to purple formazan crystals by the mitochondrial enzymes of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Guangzhou Chemical (3 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Charcoal stripped fbs, by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 hydroxytamoxifen, by Merck Group (1 mentions)
Fulvestrant, by Merck Group (1 mentions)
Bouin s solution, by Phygene (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Trypsin, by Solarbio (1 mentions)
Crystal violet, by Solarbio (1 mentions)
U0126, by MedChemExpress (1 mentions)
Penicillin streptomycin pen strep, by Solarbio (1 mentions)
β actin 4970, by Cell Signaling Technology (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Meilun (1 mentions)
Ldh cytotoxicity detection kit, by Beyotime (1 mentions)
Fluostar omega plate reader, by BMG Labtech (1 mentions)
Egg lecithin e80, by Lipoid (1 mentions)
7 protocols using methylthiazolyldiphenyl tetrazolium bromide mtt
1
Characterization of Fucoidan from F. vesiculosus
2
Isolation and Characterization of Ginseng Polysaccharides
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Five-year-old ginseng was produced in Jian City, Jilin Province, China. It was identified by Professor Wang Shumin (College of Pharmacy, Changchun University of Chinese Medicine). The samples were stored in Jilin Ginseng Academy, Changchun University of Chinese Medicine.
Sepharose CL-6B was purchased from Cytiva Co., Ltd. (Washington, United States). Monosaccharide standard Galactose (Gal), Galacturonic acid (GalA), Glucose (Glc), Glucuronic acid (GlcA), Arabinose (Ara), Rhamnose (Rha), Mannose (Man), Xylose (Xyl), Fucose (Fuc) were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). lipopolysaccharide (LPS), Concanavalin A (ConA) were obtained from Sigma Chemical Co. (St. Louis, United States). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Biotechnology (Shanghai, China). Nitric Oxide (NO) test kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
Sepharose CL-6B was purchased from Cytiva Co., Ltd. (Washington, United States). Monosaccharide standard Galactose (Gal), Galacturonic acid (GalA), Glucose (Glc), Glucuronic acid (GlcA), Arabinose (Ara), Rhamnose (Rha), Mannose (Man), Xylose (Xyl), Fucose (Fuc) were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). lipopolysaccharide (LPS), Concanavalin A (ConA) were obtained from Sigma Chemical Co. (St. Louis, United States). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Biotechnology (Shanghai, China). Nitric Oxide (NO) test kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
3
Breast Cancer Cell Line Characterization
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MCF-7 is a human breast cancer cell line. LCC2 and LCC9 are two derivatives of MCF-7 cell line, which are tamoxifen resistant and dual tamoxifen and fulvestrant resistant, respectively. MCF-7, LCC2, and LCC9 were kindly provided by Robert Clarke (Georgetown University Medical Center, Washington, DC, USA). MEM, phenol red free IMEM and FBS were purchased from Gibco. Charcoal-stripped FBS was purchased from Biological Industries. 4-Hydroxytamoxifen and fulvestrant were purchased from Sigma-Aldrich. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime. PCR primers were synthesized by Sangon Biotech.
4
Ginsenoside Rh3 Cellular Effects
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Ginsenoside Rh3 ([HPLC] ≥ 98%) was obtained from the Biomedical Research Institute of Northwest University (Xi’an, Shaanxi, China—lot number: 20,210,723; validity period extending until July 2023). RPMI 1640 was purchased from HyClone (Logan, UT, USA). Methylthiazolyldiphenyl tetrazolium bromide (MTT) and cobalt chloride (CoCl2) were purchased from Beyotime Biotechnology (Shanghai, China). Trypsin, penicillin–streptomycin (pen–strep), and crystal violet were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). U0126 was purchased from MedChemExpress. Bouin’s solution was purchased from Phygene (Fuzhou, Fujian, China). The list of antibodies used is shown in Supplementary Table S1 .
5
Nanomedicine Targeting Amino Acid Transporters
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Lapatinib was obtained from Aladdin Biochemical Co., Ltd. (Shanghai, China). JPH203 was obtained from AZBIOCHEM Co., Ltd. (Shanghai, China). DSPE-PEG2000 and DSPE-PEG2000-NHS were purchased from AVT (Shanghai) Pharmaceutical Co., Ltd. Annexin V-fluorescein isothiocyanate (FITC), propidium iodide (PI), and methylthiazolyldiphenyl-tetrazolium bromide (MTT) were purchased from Beyotime Biotechnology Co., Ltd. Primary antibodies to mTOR (7C10), p-mTOR (D9C2), Bax (2772S), Bcl-2 (3498S), and β-actin (4970S) were purchased from Cell Signaling Technology Inc. (Denver, CO, USA). Primary antibody to SLC6A14 (99102) was purchased from Abcam (Shanghai, China). Primary antibody to SLC7A5 (DF8065) was purchased from Affinity Biosciences (Pottstown, PA, USA). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) and l -Glycine were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). l -Lysine (56-87-1), l -Glutamine (56-85-9) and 2-Aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) were obtained from Aladdin Biochemical Co., Ltd. (Shanghai, China). L-Tryptophan (231123) was obtained from J&K Scientific Co., Ltd. (Beijing, China). All other chemicals and reagents were of analytical grade.
6
Evaluating Ferroptosis Cytotoxicity in MM Cells
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The methylthiazolyldiphenyl-tetrazolium bromide (MTT) (Beyotime, Shanghai, China) and lactate dehydrogenase (LDH) assays were used to evaluate MM cell proliferation and cytotoxicity. A total of 2 × 104 cells/well were seeded in a 96-well plate, and a series of increasing concentrations of SHK or ferroptosis inhibitors was added to the wells; the plate was then incubated in a 37°C incubator (5% CO2) for 24 h. Finally, 20 μL MTT solution (5 mg/mL) was added to each well. The plate was then incubated at 37°C for 3 h before measuring the absorbance of each well at 570 nm using FLUOstar Omega plate reader (BMG Labtech, Offenburg, Germany). The extent of cellular injury was determined by the amount of LDH released into the medium using an LDH cytotoxicity detection kit (Beyotime) according to the manufacturer’s instructions.
7
Cytarabine-Loaded Nanoparticle Formulation
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Cytarabine (purity 99.6%, MW 279.68) was purchased from Peking University Pharmaceutical Co., Ltd. (Beijing, China). Egg lecithin (E80) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Sodium Cholesteryl sulfate (SCS, MW 488.7) was obtained from Hubei Saibo Medical Chemistry Goods Limited Company; Octadecylamine (ODA, MW 269.51) was supplied by Sigma International Inc. ε-polylysine (PLL, molecular weight 4000kDa) was purchased from Lanzhou Weiri Bioengineering Limited Company. γ-Polyglutamic acid (PGA, molecular weight 1200kDa) was obtained from Zhejiang Haining City Biotechnology Violet Gold Harbour Limited Company. Sodium sulfite was supplied by Nanjing Longyan Chemical Co., Ltd. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Institute of Biotechnology. Fluorescein isothiocyanate (FITC) was obtained from Sigma International Inc. Paraformaldehyde was purchased from Guangzhou Chemical Reagent Factory. Chromatographic grade methanol was purchased from Concord Technology (Tianjin) Co., Inc. Water was purified by a Barnstead EASYpure® II RF/UV ultrapure water system (Dubuque, Lowa, USA). All other materials were of analytical grade.
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