TRIzol reagent was purchased from Gibco (Grand Island, NY, USA),
RT-PCR kit was purchased from Promega Corp. (Madison, WI, USA), heat-resistant DNA polymerase and ELF5 gene primer was compounded by Sangon Biotech Co., Ltd. (Shanghai, China). DNA endonuclease,
DNA ligase and
DNA marker were purchased from Fermentas (Glen Burnie, MD, USA).
DNA gel extraction kit and
agarose were purchased from Tiangen Biotech (Beijing) Co., Ltd. (Beijing, China). Medium extraction kit was purchased from Axygen Biosciences Co., Ltd. (Hangzhou, China).
Absolute ethyl alcohol,
isopropanol,
glycerol and
anhydrous calcium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Human ovarian carcinoma SKOV3 cells were purchased and stored by the Laboratory of Obstetrics and Gynecology Department of the Affiliated Hospital of Xuzhou Medical University.
Methyl thiazolyl tetrazolium (MTT) was the product of Sigma-Aldrich (St. Louis, MO, USA). Liptapfector lipofectin transfection reagent was provided by Beyotime Biotech (Jiangsu, China). Transwell chamber and ELF5 primary antibody (dilution, 1:1,000; cat. no. AM254515) was provided by Chemicon (Temecula, CA, USA).
Escherichia coli DH5α was purchased and stored by the Laboratory of Obstetrics and Gynecology Department of the Affiliated Hospital of Xuzhou Medical University.
Yan H., Qiu L., Xie X., Yang H., Liu Y., Lin X, & Huang H. (2017). ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells. Oncology Reports, 37(3), 1412-1418.