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24 protocols using anhydrous calcium chloride

1

Aqueous Inorganic Chemical Synthesis

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Sodium
chloride (NaCl,
AR), anhydrous calcium chloride (CaCl2, AR), zinc chloride
(ZnCl2, AR), anhydrous ferric chloride (FeCl3, AR), paraffin liquid (CAS: 8012-95-1, AR), 2,2,4-trimethylpentane
(C8H18, AR), and hydrochloric acid (HCl, AR,
37 wt %) were purchased from Sinopharm Chemical Reagent Co. (Shanghai,
China) and sodium lignin sulfonate (SLS, AR, 96%) was bought from
Aladdin Reagent Co. (Shanghai, China).
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2

Multicomponent Food Colorant Formulation

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Sea bass, pork tenderloin, and soybean (Taiwan 292) were purchased from a local supermarket. Milk was purchased from Mengniu Dairy Co., Ltd. Carminic acid (~85%) and carminic aluminum lake were obtained from Tongyi Biological Technology Co., Ltd. Whey protein isolate (WPI) (~92%) was purchased from Davisco Foods International Inc. Casein was purchased from Fonterra Co‐operative Group. Monascus color was obtained from Yinuo Biotechnology Co., Ltd. Disodium phosphate dodecahydrate, citric acid, iron (III) chloride hexahydrate, ferrous chloride tetrahydrate, anhydrous calcium chloride, copper (II) sulfate pentahydrate, sodium chloride, potassium chloride, sodium nitrite, and ascorbic acid were purchased from Sinopharm Chemical Reagent Co., Ltd.
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3

Stimuli-Responsive Alginate-Based Hydrogels

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Sodium alginate powder (Alg), sodium acrylate (SA) and methyl acrylate (MA) were purchased from Aladdin Industrial Corporation; N,N′-methylenebisacrylamide (MBAA), dichloromethane (CH2Cl2), α-methacrylic acid (MAA), ammonium persulfate (APS), Tween 80 and anhydrous calcium chloride (CaCl2) were supplied by Sinopharm Chemical Reagent Co., Ltd., Beijing, China. 4-Dimethylaminopyridine (DMAP), N,N′-dicyclohexylcarbodiimide (DCC) and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Aladdin Industrial Corporation, Shanghai, China. 1-(2-Hydroxyethyl)-3,3-dimethylindolino-6′-nitrobenzopyrylospiran (SPOH) was supplied by TCI (Shanghai) Development Co., Ltd., Shanghai, China. Deionised water was used in all experiments (18.2 Ω cm resistivity at 25 °C).
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4

Synthesis of Graphene Oxide-Alginate Composites

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Graphene oxide (GO, membrane diameter 0.5–5 m) was purchased from XFNANO Ltd (Nanjing, China). Sodium alginate (SA, CP), 4-aminobenzenesulfonic acid (AR), hydrochloric acid (GR), and absolute ethanol (AR) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Anhydrous calcium chloride (AR), sodium nitrite (AR) and sodium hydroxide (AR) were supplied by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Nylon micro-filtration membranes (pore size 0.2 μm) were purchased from Tianjin Jinteng Experiment Equipment Co., Ltd (Tianjin, China).
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5

Maize Straw-Derived Magnetite Adsorbent

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The powder of maize straws was obtained from local suppliers. Magnesium chloride hexahydrate (MgCl2·6H2O, AR) and sodium hydroxide (NaOH, AR) were provided by Xilong Science Co., Ltd. (Guangdong, China). Ascorbic acid (AS, >99.0%) and iron (II, III) oxide (Fe3O4, 97%) were provided by Aladdin (Shanghai, China). Ammonium molybdate ((NH4)2MoO4, AR), L-antimony potassium tartrate (C8H4K2O12Sb2·xH2O, 99%), and potassium dihydrogen phosphate (KH2PO4, >99.5%) were provided by Macklin (Shanghai, China). Sulfuric acid (H2SO4, AR), sodium alginate (SA, CP), and anhydrous calcium chloride (CaCl2, AR) were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All chemicals were used without further purification. Deionized water was applied in all experiments.
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6

Biopolymer Hemostatic Dressing Protocol

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Carboxymethyl chitosan, sodium alginate, tranexamic acid, glycerin, and anhydrous calcium chloride were all purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Unless otherwise specified, all reagents were of analytical grade.
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7

Gadolinium-based Contrast Agent Synthesis

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Anhydrous calcium chloride, anhydrous sodium carbonate, and ethyl alcohol were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai). Gadolinium chloride hexahydrate (GdCl3·6H2O, 99.95%) were purchased from Yutai QingDa Fine Chemical Co., Ltd. (Shandong, China). Poly(acrylic acid) (PAA, Mw ≈ 1800) was purchased from Aldrich. All reagents were used as received without further purification. The commercially used Gd-DTPA was produced by Guangdong Consun Pharmaceutical Group, China (specification: 20 mL).
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8

Synthesis and Characterization of Hydrogel

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The main agent of the gel was partially hydrolyzed polyacrylamide (HPAM) with a 25% hydrolysis degree and an average molecular weight of 12 × 106 g/mol supplied by Hebei Liancheng Environmental Protection Technology Co., Shijiazhuang, China. The crosslinker was a water-soluble phenolic resin (WSPR) solution with an effective content of 30% provided by Dongying Tongkun Technology Co., Dongying, China. The nano-SiO2 solution with an effective solid content of 30% and a median particle size of 15 nm for nanoparticles was purchased from Dongying Liuhe Co., Dongying, China. Analytically pure hydroquinone provided by Sinopharm Chemical Reagent Co., Shanghai, China, was also used in the experiment. In addition, analytically pure drugs, such as sodium chloride, potassium chloride, anhydrous calcium chloride, magnesium chloride hexahydrate, sodium bicarbonate, and sodium sulfate from Sinopharm Chemical Reagent Co., Shanghai, China, were used in this study to prepare synthetic saline for the preparation of gels.
To simulate the salinity conditions of formation water in the reservoir, a synthetic brine with a salinity of 12.124 g/L was synthesized using inorganic salts to formulate a gelant. The composition of the synthetic brine is shown in Table 2.
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9

Emulsified Asphalt for Construction

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The dimeric acid (95 wt%) was purchased from Shanghai Maclean Biochemical Technology, Shanghai, China, and the 1,6-hexanediamide was purchased from Shanghai Aladdin Biochemical Technology Co. Ltd., Shanghai, China. The Span80 (98 wt%), calcium oxide (96 wt%), and anhydrous calcium chloride (96 wt%) were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, and the #5 white oil was obtained from Shenzhen ZRT Chemical Industry, Shenzhen, China. The industrial-grade organoclay, blown asphalt, and barite were obtained from Hubei Hanc New-Technology Co., Ltd., Jingzhou, China. The main emulsifier (modified fatty acid) and secondary emulsifier (fatty acid polyamine) were prepared in the laboratory.
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10

Ovarian Cancer SKOV3 Cells Analysis

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TRIzol reagent was purchased from Gibco (Grand Island, NY, USA), RT-PCR kit was purchased from Promega Corp. (Madison, WI, USA), heat-resistant DNA polymerase and ELF5 gene primer was compounded by Sangon Biotech Co., Ltd. (Shanghai, China). DNA endonuclease, DNA ligase and DNA marker were purchased from Fermentas (Glen Burnie, MD, USA). DNA gel extraction kit and agarose were purchased from Tiangen Biotech (Beijing) Co., Ltd. (Beijing, China). Medium extraction kit was purchased from Axygen Biosciences Co., Ltd. (Hangzhou, China). Absolute ethyl alcohol, isopropanol, glycerol and anhydrous calcium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Human ovarian carcinoma SKOV3 cells were purchased and stored by the Laboratory of Obstetrics and Gynecology Department of the Affiliated Hospital of Xuzhou Medical University. Methyl thiazolyl tetrazolium (MTT) was the product of Sigma-Aldrich (St. Louis, MO, USA). Liptapfector lipofectin transfection reagent was provided by Beyotime Biotech (Jiangsu, China). Transwell chamber and ELF5 primary antibody (dilution, 1:1,000; cat. no. AM254515) was provided by Chemicon (Temecula, CA, USA). Escherichia coli DH5α was purchased and stored by the Laboratory of Obstetrics and Gynecology Department of the Affiliated Hospital of Xuzhou Medical University.
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