The rat heart fixed in 4% paraformaldehyde was removed and dehydrated in different concentrations of alcohol. Then, the dehydrated sample was embedded in paraffin. Continuous dyeing was presented according to the instructions of H&E Staining Kit (Solarbio, #G1120). The stained myocardial sample was scanned with a digital slice scanner (Aperio VERSA; Leica).
Aperio versa
Manufactured by Leica
Sourced in Germany, United States
The Aperio Versa is a digital slide scanner that captures high-resolution images of microscope slides. It is designed to digitize glass slides for use in various applications, such as pathology, research, and education.
Automatically generated - may contain errors
Lab products found in correlation
Diaminobenzidine dab, by Vector Laboratories (2 mentions)
Avidin biotin peroxidase complex, by Vector Laboratories (2 mentions)
He staining kit, by Solarbio (1 mentions)
Living image 4, by PerkinElmer (1 mentions)
Chemidot mp imaging system, by Bio-Rad (1 mentions)
Prism 9, by GraphPad (1 mentions)
Prussian blue iron stain kit, by Solarbio (1 mentions)
In situ apoptosis detection kit, by Abcam (1 mentions)
Bluing reagent, by Roche (1 mentions)
Chromomap dab detection kit, by Roche (1 mentions)
Red 610 kit, by Roche (1 mentions)
Dab map detection kit, by Roche (1 mentions)
Omnimap anti rabbit hrp, by Roche (1 mentions)
Aperio at2, by Leica (1 mentions)
Hematoxylin 2, by Roche (1 mentions)
Halo platform, by Indica Labs (1 mentions)
Discovery ultra, by Roche (1 mentions)
Ab54427, by Abcam (1 mentions)
Ab210515, by Abcam (1 mentions)
Ab33661, by Abcam (1 mentions)
29 protocols using aperio versa
1
Histological Analysis of Rat Myocardium
2
Multimodal Imaging and Analysis Techniques
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Graphs and boxplots were created using Graphpad Prism 9 software for Windows, GraphPad Software, San Diego, CA, USA. Aperio Versa by Leica was used to capture IHC images. QuPathv0.1.3, open-source software was used for IHC analysis and quantification. The Xenogen IVIS instrument (Perkin Elmer, Waltham, MA, USA) was used for bioluminescence imaging of mice and imaging analysis was performed using Living Image 4.7.3. (Perkin Elmer, Waltham, MA, USA). A Clariostar plate reader (Mutli-user SMART Control and MARS data analysis software v6.0, BMG LABTECH GmbH, Ortenberg, Germany) was used to measure absorbance for ECM adhesion assay. A Chemidot MP imaging system by Biorad or a Simple western Jess instrument (bio techne, Minneapolis, MN, USA) was used for immunoblotting detection. Incucyte® Live Imaging and Analysis System was used for cell proliferation. Schematic illustrations were made using Biorender.com, accessed on June 2023.
3
Tissue Imaging Protocol for Murine and Human Samples
4
Histological Analysis of Mouse Liver
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Mice were sacrificed and the livers were immobilized with 4% FPA and embedded in paraffin. Tissues were cut into 4.5-um sections and stained with hematoxylin and eosin (H&E) as previously described (Li, Liu, Wu, & Li, 2020 (link)). The images of all slides were captured by Aperio Versa (Leica, Wetzlar, Germany).
5
Histological Analysis of Mouse Liver
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For hematoxylin and eosin (H&E) staining, mice liver tissues were fixed with 4% formaldehyde and prepared for paraffin sections. After dewaxed, paraffin sections were rehydrated, stained with hematoxylin and eosin, respectively. The images of all sections were captured by Aperio Versa (Leica, Wetzlar, Germany).
6
Liver Histological Analysis Protocol
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Mice were sacrificed and the livers were immobilized with 4% FPA and embedded in paraffin. Tissues were cut into 4.5-μm sections and stained with hematoxylin and eosin (H&E) and Masson trichrome staining as previously described (Li, Li, Xue, Wang, & Li, 2022 ). After dewaxing and rehydration, tissue sections were stained with Van Gieson's Stain Kit (RS3930) and imaged with Aperio Versa (Leica, Wetzlar, Germany).
7
Quantifying Liver Iron via Prussian Blue
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To measure the total iron in the liver, liver sections and AML12 cells were stained with a Prussian blue iron stain kit (G1422, Solarbio, Beijing, China). Following the manufacturer's instructions, the samples were incubated with Perls staining buffer at least 30 min before being examined and captured under an Aperio Versa (Leica, Wetzlar, Germany).
8
Oil-red O Staining Protocol
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Oil-red O power was dissolved in isopropanol (0.5 g/mL) and filtered with the filter paper for two times to obtain the stock solution of Oil-red, which was then diluted with ddH2O for further staining. Fixed HepG2 cells and fresh frozen liver sections were washed with PBS for three times and then stained with diluted Oil-red O solution for 30 min. The stained cells and sections were imaged by Aperio Versa (Leica, Wetzlar, Germany).
9
Oil-red Staining of Liver Tissue
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Oil-red staining was performed as previous established conditions (Li et al., 2021 (link)). Oil-red powder was first dissolved in isopropanol solution (0.5 g/ml). Freshly cut sections from frozen livers and hepatocytes were fixed in 4% formaldehyde, washed in PBS solution and subsequently stained by Oil-red solution for 30 min. The image was captured by Aperio Versa (Leica, Wetzlar, Germany).
10
Apoptosis and Acute Neuronal Injury Assessment
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After the scheduled surgical resection of the anterior-mesial temporal lobe [30] , the removed tissue was fixed in 10% formaldehyde, embedded in paraffin, and sectioned at 10 mm. Apoptosis assays were performed using the In situ Apoptosis Detection kit per manufacturer's instructions (Abcam). Briefly, sections were deparaffinized and subsequently treated with terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) and counterstained with methyl green. As a positive control, non-sonicated tissue was treated with DNase I to generate free 3 0 -OH ends for TUNEL labeling.
To visualize potential damage due to sonication, adjacent paraffin sections were stained with either hematoxylin and eosin (H&E), or vanadium acid fuchsin (VAF) with toluidine blue counterstain. H&E staining is customarily used for examination of tissue integrity, whereas VAF-toluidine blue staining is performed to detect the presence of acidophilic neurons which are indicative of acute neuronal injury and subsequent apoptosis or necrosis. VAF staining also allows for the visualization of extravasation and blood vessel disruption [31] . The samples were imaged at the UCLA Translational Pathology Core Laboratory (TPCL) using Applied Imaging Leica Aperio Versa.
To visualize potential damage due to sonication, adjacent paraffin sections were stained with either hematoxylin and eosin (H&E), or vanadium acid fuchsin (VAF) with toluidine blue counterstain. H&E staining is customarily used for examination of tissue integrity, whereas VAF-toluidine blue staining is performed to detect the presence of acidophilic neurons which are indicative of acute neuronal injury and subsequent apoptosis or necrosis. VAF staining also allows for the visualization of extravasation and blood vessel disruption [31] . The samples were imaged at the UCLA Translational Pathology Core Laboratory (TPCL) using Applied Imaging Leica Aperio Versa.
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