Humanomniexpress 12v1.1 arrays

Genomic DNA was extracted from venous blood using either the phenol–chloroform extraction method or automated nucleic acid purification using paramagnetic particles (Freedom EVO^® Nucleic Acid Purification Workstation). The quality and quantity of purified genomic DNA were evaluated with a spectrophotometer (NanoDrop^® ND‐1000 Spectrophotometer). Genome‐wide genotyping following manufacturer's protocols was performed using high‐density Illumina HumanOmniExpress‐12v1.1 arrays (719,666 SNPs) on an Illumina HiScan^™SQ system.

Genomic DNA was extracted from whole venous blood using either the phenol-chloroform extraction method or the automated DNA extraction platform TECAN Freedom EVO (TECAN Group Ltd., Männedorf, Switzerland) based on the paramagnetic particle method. DNA concentration and quality were measured by a NanoDropR ND-1000 spectrophotometer (NanoDrop Technologies Inc., USA).
SNP genotyping of 254 samples was performed with Illumina HumanOmniExpress-12 v1.1 arrays at the Department of Human and Medical Genetics, Faculty of Medicine, Vilnius University, Lithuania, while the other 32 samples underwent this procedure with a HumanOmniExpress-12 v1.0 (Illumina, San Diego, CA, USA) at the University of Tartu, Estonia using the standard Illumina Infinium® HD Assay Ultra protocol recommended by the manufacturer (Catalog # WG-901–4005).
Genotyping data quality control was performed according to the standard recommendations by the manufacturer. Individuals with a call rate <98 % and a standard deviation (SD) of the log R ratio (LRR) of >0.3 were excluded from further analysis.