Laser bench

All microscopy experiments were performed on an inverted Zeiss Axio Observer (Carl Zeiss Inc.) equipped with a Plan-Apochromat 63X/1.4 NA immersion lens (Carl Zeiss Microscopy GmbH, Jena, Germany), a Lumencor Spectra X illumination system (Lumencor Inc., Beaverton, Oregon), a laser bench (Visitron Systems GmbH, Puchheim, Germany), and a Yokogawa CSU-W1 spinning disc confocal head (Yokagawa Life Science, Japan). Images were acquired using a Hamamatsu Orca Flash 4.0V2 sCMOS camera (Hamamatsu Photonics, Hamamatsu, Japan) and the Visiview software (Visitron Systems GmbH, Puchheim, Germany). Analyses were performed using Fiji (National Institutes of Health, USA).

Imaging for the results presented in Figs. 2c-e, 3b-d, 4a-b, 5a-b and 6a was performed using an inverted Zeiss Axio Observer (Carl Zeiss Inc.) equipped with a Lumencor Spectra X illumination system and a Hamamatsu Orca Flash 4.0 V2 sCMOS camera. The data for Figs. 1f and 3c were obtained using a similar setup that is equipped with a spinning disc confocal head and a laser bench (Visitron GmbH). For both systems, images were acquired using Visiview (Visitron GmbH). The images displayed in Figs. 1c-e (bottom panel) and 4c were acquired using a Zeiss Axio Vert. A1 (Carl Zeiss Inc.) equipped with a Nikon 1 J3 camera and a PlasDIC system. All image analyses were performed using Fiji (National Institutes of Health).