Sinomenine was purchased from Solarbio Life Sciences (#SS8560,Solarbio, Beijing, China), dissolved in DMSO and placed away from light at −20°C. Anti-MARCH1 antibody (#bs-9335R) was obtained from Bioss (Beijing, China). Anti-p-STAT3 (#ab32143), p-AMPK (#ab92701) and AMPK (#ab32047) antibodies were purchased from Abcam (Cambridge, United Kingdom). Anti-Bcl-2 (#12789-1-AP), CyclinB1 (#55004-1-AP), CyclinD1 (#26939-1-AP), Mcl-1 (#16225-1-AP), Bax (#50599-2-Ig), STAT3 (#10253-2-AP), GAPDH (#10494-1-AP) and Peroxidase-conjugated Affinipure Goat anti-Rabbit IgG (H + L) (#SA00001-2) antibodies were purchased from the Proteintech Group (Chicago, IL, United States).
Peroxidase conjugated affinipure goat anti rabbit igg h l sa00001 2
Manufactured by Proteintech
Sourced in United States
Peroxidase-conjugated Affinipure Goat anti-Rabbit IgG (H + L) (#SA00001-2) is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with the enzyme peroxidase, which can be used for detection in immunoassays, such as Western blotting and ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Mcl 1, by Proteintech (1 mentions)
Anti bcl 2, by Proteintech (1 mentions)
Sinomenine, by Solarbio (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cyclin b1, by Proteintech (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Anti β actin 20536 1 ap, by Proteintech (1 mentions)
2 protocols using peroxidase conjugated affinipure goat anti rabbit igg h l sa00001 2
1
Sinomenine Modulates STAT3 and AMPK
2
Immunofluorescence Protein Detection
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The following primary antibodies were used: anti-P11 (AF2377, R&D Systems, Minneapolis, USA), anti-β-actin (20536-1-AP, Proteintech Group, Chicago, USA) and Bromodeoxyuridine (BrdU) antibody (GTX128091, GeneTex, Texas, USA). The following second antibodies were used: peroxidase-conjugated affinipure rabbit anti-goat IgG (H+L) (SA00001-4, Proteintech Group), peroxidase-conjugated affinipure goat anti-rabbit IgG (H+L) (SA00001-2, Proteintech Group) and TRITC-conjugated goat anti-rabbit IgG (H+L) (T-2769, Thermo Fisher, Massachusetts, USA).
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