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Human 293T is a cell line derived from human embryonic kidney (HEK) cells. It is a commonly used cell line in cell biology and molecular biology research. The 293T cell line expresses the large T antigen of SV40, which allows for increased transfection efficiency and protein expression.

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Lab products found in correlation

Freestyle 293 expression medium, by Thermo Fisher Scientific (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

HEK-293T human epithelial kidney cells Human embryonic kidney 293T 293t human embryonic kidney cell line (ATCC® CRL-3216™) 293T human embryonic kidney cell line Human 293T cells Human 293T cell lines Human embryonic kidney 293T cells 293T human embryonic cells

4 protocols using human 293t

1

Cell Line Cultivation Conditions

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Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 expression medium (Thermo Fisher) supplemented with penicillin and streptomycin. Human 293T (ATCC CRL-3216; American Type Culture Collection), green monkey Vero (ATCC CCL-81), and rhesus macaque LLC-MK2 (ATCC CCL-7) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and maintained at 37°C and 5% CO2. Owl monkey OMK cells (ATCC CRL-1556) were grown in Eagle’s minimum essential medium (EMEM) supplemented with 10% FBS and maintained at 37°C and 5% CO2.
McCarthy K.R., Timpona J.L., Jenni S., Bloyet L.M., Brusic V., Johnson W.E., Whelan S.P, & Robinson-McCarthy L.R. (2020). Structure of the Receptor Binding Domain of EnvP(b)1, an Endogenous Retroviral Envelope Protein Expressed in Human Tissues. mBio, 11(6), e02772-20.
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2

ACD Treatment Induces Transcriptional Changes

Cited 3 times
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Human Huh7 (Health Science Research Resources Bank JCRB0403), mouse Hepa-1 (ATCC CRL-1830) and human 293T (ATCC CRL-3216) were maintained as described previously (37 (link), 41 (link)). For ACD treatment, exponentially growing cells were treated with 500 ng/ml ACD for 0, 30 and 60 min. Standard methods were used for RNA and protein isolation, cDNA synthesis, qPCR, WB, transient transfection, and luciferase reporter assay (42 (link), 43 (link)). Experiments were done in triplicate from three independent experiments.
Zhang L., Yang Z., Trottier J., Barbier O, & Wang L. (2016). LncRNA MEG3 induces cholestatic liver injury by interaction with PTBP1 to facilitate Shp mRNA decay. Hepatology (Baltimore, Md.), 65(2), 604-615.
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3

Culturing Human and Mouse Macrophages

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Human 293T was obtained from American Type Culture Collection (Manassas, VA). C57BL/6 mice-derived iBMDM cells were gift from Feng Shao (NIBS)31 (link). The cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% 56 °C complement inactivated fetal bovine serum (FBS), and 1% (v/v) penicillin-streptomycin solution. For ligand stimulation, cells were washed once with PBS, and incubated with 100 ng/mL LPS at indicated times.
Liu Q., Zhang S., Chen G, & Zhou H. (2017). E3 ubiquitin ligase Nedd4 inhibits AP-1 activity and TNF-α production through targeting p38α for polyubiquitination and subsequent degradation. Scientific Reports, 7, 4521.
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4

Authenticated Cell Lines for Breast Cancer

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Human breast cancer cell lines,T47D, MCF7, BT549, HS578T, and human 293T (ATCC Cat# CRL-3216, RRID:CVCL_0063) were obtained from American Type Culture Collection. The identities of all cell lines were confirmed by the medical genome facility at Mayo Clinic using short tandem repeat profiling upon receipt. Mycoplasma tests were performed using the MycoAlert Mycoplasma Detection Kit (Lonza, LT07–318). Cells were passaged <20 times. The anastrozole resistant MCF7/AnaR cells were obtained from the European Collection of Authenticated Cell Cultures (15 (link)).
Cairns J., Ingle J.N., Kalari K.R., Goetz M.P., Weinshilboum R.M., Gao H., Li H., Bari M.G, & Wang L. (2021). Anastrozole regulates fatty acid synthase in breast cancer. Molecular cancer therapeutics, 21(1), 206-216.
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