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Anti pum1

Manufactured by Abcam
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Anti-PUM1 is a polyclonal antibody that recognizes the PUM1 protein. PUM1 is an RNA-binding protein that plays a role in the regulation of gene expression and mRNA translation. The antibody can be used to detect and study the PUM1 protein in various applications.

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Lab products found in correlation

Anti brdu antibody, by Thermo Fisher Scientific (2 mentions) Hartman s fixative, by Merck Group (2 mentions) In situ cell death detection kit, by Roche (2 mentions) Anti cdkn1b, by Abcam (2 mentions) Anti β actin, by Merck Group (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg hrp, by Merck Group (1 mentions) Donkey anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti ddk, by OriGene (1 mentions)

3 protocols using anti pum1

1

Histological Analysis of Cell Proliferation

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Tissues were fixed overnight in Hartman’s fixative (Sigma) and processed for H&E staining and immunohistochemistry according to standard protocols (VanGompel and Xu, 2010 (link)). Immunostaining for PUM1, CDKN1B, phospho-Histone H3 were performed, following citrate buffer antigen retrieval, by incubation with anti-PUM1(1/50, Abcam), anti-CDKN1B (1/50, Abcam), and anti-phospho-H3 (1/50, CST) primary antibodies and detected using Biotin-Streptavidin HRP Detection Systems (ZSGB-BIO). For BrdU incorporation experiments, mice were injected intraperitoneally with 50 mg/kg BrdU in PBS and sacrificed 2 hr later, and tissue sections were analyzed by immunohistochemistry with anti-BrdU antibody (Invitrogen). TUNEL analysis was performed using the In Situ Cell Death Detection Kit from Roche according to the manufacturer’s instructions. A minimum of three randomly chosen discontinuous sections were used to determine positive cells in tubules.
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
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2

Western Blot Antibody Detection Protocol

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This study used the following primary antibodies: anti-DDK (OriGene Technologies, TA50011, USA) for detection of proteins in the pCMV6-entry vector system 1:2500, anti-β-ACTIN (Sigma Aldrich, A2066) 1:10000, anti-SPIN1 (Abcam, Ab118784, UK) 1:1000, anti-PUM1 (Abcam, Ab3717) 1:5000, anti-PUM2 (Santa Cruz Biotechnology, sc-31535) 1:500. Secondary antibodies included: donkey anti-goat IgG-HRP (Santa Cruz Biotechnology, sc-2020) 1:50000, goat anti-rabbit IgG-HRP (Sigma Aldrich, A6154) 1:25000, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, sc-2005) 1:10000.
Janecki D.M., Sajek M., Smialek M.J., Kotecki M., Ginter-Matuszewska B., Kuczynska B., Spik A., Kolanowski T., Kitazawa R., Kurpisz M, & Jaruzelska J. (2018). SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget, 9(65), 32466-32477.
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3

Histological Analysis of Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were fixed overnight in Hartman’s fixative (Sigma) and processed for H&E staining and immunohistochemistry according to standard protocols (VanGompel and Xu, 2010 (link)). Immunostaining for PUM1, CDKN1B, phospho-Histone H3 were performed, following citrate buffer antigen retrieval, by incubation with anti-PUM1(1/50, Abcam), anti-CDKN1B (1/50, Abcam), and anti-phospho-H3 (1/50, CST) primary antibodies and detected using Biotin-Streptavidin HRP Detection Systems (ZSGB-BIO). For BrdU incorporation experiments, mice were injected intraperitoneally with 50 mg/kg BrdU in PBS and sacrificed 2 hr later, and tissue sections were analyzed by immunohistochemistry with anti-BrdU antibody (Invitrogen). TUNEL analysis was performed using the In Situ Cell Death Detection Kit from Roche according to the manufacturer’s instructions. A minimum of three randomly chosen discontinuous sections were used to determine positive cells in tubules.
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
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