We used a mixture comprising of 40% methanol (LC–MS grade, CNW Technologies): 40% acetonitrile (LC–MS grade, CNW Technologies): 20% water (v: v: v) as an extract solvent. The ratio was 0.05 g of leaves (each sample set consisted of six biological replicates) to 1 mL of solvent, and the solutions were vortexed for 30 s. The samples were homogenized (45 Hz, 4 min, JXFSTPRP-24; Jingxin Technology), and sonicated (5 min in ice-water bath). This step was repeated three times, after which the samples were left to stand at − 20 °C for 1 h, and underwent centrifugation (Heraeus Fresco17; Thermo Fisher Scientific) for 15 min at 12000 rpm at 4 °C. The supernatants were introduced to LC-MS vials for UHPLC-QE Orbitrap/MS detection. Equivalent amounts of supernatants from all samples were mixed as quality-control (QC) samples for testing.
Lc ms grade
Manufactured by CNW Technologies
Sourced in Germany
LC-MS grade is a type of laboratory equipment designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is specifically manufactured to meet the rigorous standards required for LC-MS analysis, ensuring reliable and consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
Jxfstprp 24, by Jingxin (5 mentions)
Ethyl acetate, by Tianjin Damao (3 mentions)
Ethanol, by Tianjin Damao (3 mentions)
2 4 dinitrofluorobenzene dnfb, by Maclin Biochemical Technology (3 mentions)
Heraeus fresco 17, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by CNW Technologies (1 mentions)
Formic acid, by CNW Technologies (1 mentions)
Methanol, by CNW Technologies (1 mentions)
Acquity uplc beh c18 column, by Waters Corporation (1 mentions)
8 protocols using lc ms grade
1
Metabolite Extraction and Profiling Protocol
2
Metabolite Extraction from Plant Leaves
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We used a mixture comprising of 40% methanol (LC-MS grade, CNW Technologies): 40% acetonitrile (LC-MS grade, CNW Technologies): 20% water (v: v: v) as an extract solvent. The ratio was 0.05 g of leaves (each sample set consisted of six biological replicates) to 1 mL of solvent, and the solutions were vortexed for 30 s. The samples were homogenized (45 Hz, 4 min, JXFSTPRP-24; Jingxin Technology), and sonicated (5 min in ice-water bath). This step was repeated three times, after which the samples were left to stand at -20°C for one hour, and underwent centrifugation (Heraeus Fresco17; Thermo Fisher Scienti c) for 15 min at 12000 rpm at 4°C. The supernatants were introduced to LC-MS vials for UHPLC-QE Orbitrap/MS detection. Equivalent amounts of supernatants from all samples were mixed as qualitycontrol (QC) samples for testing.
3
Metabolite Extraction and Analysis Protocol
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We used a mixture comprised of 40% methanol (LC-MS grade, CNW Technologies): 40% acetonitrile (LC-MS grade, CNW Technologies): 20% water (v: v: v) as an extract solvent. The ratio was 0.05 g of leaves (each sample set consisted of six biological replicates) to 1000 μL of solvent, and the solutions were vortexed for 30 s. The samples were homogenized (45 Hz, 4 min, JXFSTPRP-24; Jingxin Technology), and sonicated (5 min in ice-water bath). This step was repeated three times, after which the samples were left to stand at -20°C for one hour, and underwent further centrifugation (Heraeus Fresco17; Thermo Fisher Scienti c) for 15 min at 12000 rpm at 4°C. The supernatants were introduced to LC-MS vials for UHPLC-QE Orbitrap/MS detection. Equivalent amounts of supernatants from all samples were mixed as quality-control (QC) samples for testing.
4
Metabolite Extraction from Plant Leaves
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We used a mixture comprising of 40% methanol (LC-MS grade, CNW Technologies): 40% acetonitrile (LC-MS grade, CNW Technologies): 20% water (v: v: v) as an extract solvent. The ratio was 0.05 g of leaves (each sample set consisted of six biological replicates) to 1 mL of solvent, and the solutions were vortexed for 30 s. The samples were homogenized (45 Hz, 4 min, JXFSTPRP-24; Jingxin Technology), and sonicated (5 min in ice-water bath). This step was repeated three times, after which the samples were left to stand at -20°C for one hour, and underwent centrifugation (Heraeus Fresco17; Thermo Fisher Scienti c) for 15 min at 12000 rpm at 4°C. The supernatants were introduced to LC-MS vials for UHPLC-QE Orbitrap/MS detection. Equivalent amounts of supernatants from all samples were mixed as qualitycontrol (QC) samples for testing.
5
Metabolite Extraction from Plant Leaves
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We used a mixture comprising of 40% methanol (LC-MS grade, CNW Technologies): 40% acetonitrile (LC-MS grade, CNW Technologies): 20% water (v: v: v) as an extract solvent. The ratio was 0.05 g of leaves (each sample set consisted of six biological replicates) to 1 mL of solvent, and the solutions were vortexed for 30 s. The samples were homogenized (45 Hz, 4 min, JXFSTPRP-24; Jingxin Technology), and sonicated (5 min in ice-water bath). This step was repeated three times, after which the samples were left to stand at -20°C for one hour, and underwent centrifugation (Heraeus Fresco17; Thermo Fisher Scienti c) for 15 min at 12000 rpm at 4°C. The supernatants were introduced to LC-MS vials for UHPLC-QE Orbitrap/MS detection. Equivalent amounts of supernatants from all samples were mixed as qualitycontrol (QC) samples for testing.
6
Smilax glabra Roxb. Rhizome Extraction and Analysis
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The following materials were used: the TMT10pLex™ isotopic labeling reagent and kit (Thermo Scientific); a protein quantitative assay kit and a gel kit (Kangwei Biotechnology Co., Ltd., China); a 10K ultrafiltration tube (Pall Corporation, United States); formic acid, methanol, and acetonitrile (LC-MS grade, CNW Technologies); L-2-chloro-l-phenylalanine (2-chloro-L-phenylalanine, ≥98%, Shanghai Hengbo Biotechnology Co., Ltd.); and L-nitroarginine (L-NAME, Sigma Corporation, United States). ELISA kits for total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were from Shanghai Enzyme-linked Biotechnology Co., Ltd., China; ELISA kits for ALDH2 was from Shanghai Jiemei Gene Medicine Technology Co., Ltd., China; and BCA protein quantitative assay and SDS-PAGE gel kits were from Beyotime Biotechnology, China.
In April 2022, dried rhizomes of Smilax glabra Roxb., a plant belonging to the family Liliaceae, were obtained from Ziyun Miao Buyi Autonomous County, Anshun City, Guizhou Province. The botanical drug was identified by Professor Wei Shenghua of Guizhou University of Traditional Chinese Medicine, and the plant specimens were stored in this university (specimen number: TFL 2022. 04). Captopril (25 mg tablet) was obtained from Guangdong Pi Di Pharmaceutical Co., Ltd., China.
In April 2022, dried rhizomes of Smilax glabra Roxb., a plant belonging to the family Liliaceae, were obtained from Ziyun Miao Buyi Autonomous County, Anshun City, Guizhou Province. The botanical drug was identified by Professor Wei Shenghua of Guizhou University of Traditional Chinese Medicine, and the plant specimens were stored in this university (specimen number: TFL 2022. 04). Captopril (25 mg tablet) was obtained from Guangdong Pi Di Pharmaceutical Co., Ltd., China.
7
Phytochemical Profiling of TCM Herbs
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Coptis chinensis Franch.(Huanglian), Scutellaria baicalensis Georgi (huangqin), Phellodendron amurense Rupr.(huangbai), and Gardenia jasminoides J. Ellis (zhizi) were purchased from Hangzhou Mintai (Bozhou) Chinese Herbal Medicine Co. Ltd. (Zhejiang, China) and were certified by the standards elaborated in Chinese Pharmacopeia (2020 edition). Portions of the above four herbs were deposited in the Department of Pharmacy of Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang for future reference under reference number Xu1-4. 2,4-dinitrofluorobenzene (DNFB) was purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China).
MEthanol, acetonitrile, and formic acid (LC-MS grade) were purchased from CNW Technologies (Germany). Ethanol, petroleum ether, and ethyl acetate (analysis grade) were obtained from Tianjin Damao Chemical Reagent Co., Ltd. (China). The standard substances of baicalin, chrysin, ferulic acid, geniposide, obacunone, wogonin, wogonoside, auraptene, protopine, isochlorogenic acid A, chelerythrine, rutecarpine, palmatine, and berberine were purchased from the resource platform of the National Standard Material (China).
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Coptis chinensis Franch.(Huanglian), Scutellaria baicalensis Georgi (huangqin), Phellodendron amurense Rupr.(huangbai), and Gardenia jasminoides J. Ellis (zhizi) were purchased from Hangzhou Mintai (Bozhou) Chinese Herbal Medicine Co. Ltd. (Zhejiang, China) and were certified by the standards elaborated in Chinese Pharmacopeia (2020 edition). Portions of the above four herbs were deposited in the Department of Pharmacy of Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang for future reference under reference number Xu1-4. 2,4-dinitrofluorobenzene (DNFB) was purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China).
MEthanol, acetonitrile, and formic acid (LC-MS grade) were purchased from CNW Technologies (Germany). Ethanol, petroleum ether, and ethyl acetate (analysis grade) were obtained from Tianjin Damao Chemical Reagent Co., Ltd. (China). The standard substances of baicalin, chrysin, ferulic acid, geniposide, obacunone, wogonin, wogonoside, auraptene, protopine, isochlorogenic acid A, chelerythrine, rutecarpine, palmatine, and berberine were purchased from the resource platform of the National Standard Material (China).
8
Orbitrap-Based Metabolomic Analysis
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The analytical instrumentation comprised the Orbitrap Exploris 120 quadrupole-Orbitrap high-resolution mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) coupled with the Vanquish UHPLC ultra-high-performance liquid chromatography system (Thermo Fisher Scientific, USA). Chromatographic separation was achieved using an Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm particle size, Waters, Milford, MA, USA). Solvents employed included methanol, acetonitrile, and formic acid (LC-MS grade, CNW Technologies, Dusseldorf, Germany), alongside purified water.
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