Pacific blue anti human mouse ki67

Manufactured by BioLegend

Sourced in United States

The Pacific Blue anti-human/mouse Ki67 is a fluorochrome-conjugated antibody used to detect the expression of Ki67 protein, a cellular marker of proliferation, in human and mouse samples. This product is intended for research use only.

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Lab products found in correlation

2 protocols using pacific blue anti human mouse ki67

Multicolor Flow Cytometry Analysis

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Pembrolizumab and nivolumab (anti-PD-1) were obtained from Merck and Bristol-Myers Squibb. The following antibodies were used for flow cytometry analyses: FITC anti-human CD4 (BioLegend Cat#391503), PE anti-human CD45 (Biolegend, clone HI30), APC anti-human CD56 (Biolegend, clone 5.1H11), APC/Cyanine7 anti-human CD8a (Biolegend, San Diego, CA, USA, clone RPA-T8), PE/Cyanine7 anti-human CD3 (Biolegend, clone HIT3a), PerCP/Cyanine5.5 anti-human CD314 (NKG2D) (Biolegend, clone 1D11), FITC anti-human CD6 (Biolegend, clone BL-CD6), APC anti-human CD16 (Biolegend, clone 3G8), Pacific Blue anti-human/mouse Ki67 (Biolegend, clone 16A8), FITC anti-human/mouse Granzyme B (Biolegend, clone GB11) and APC anti-human Perforin (Biolegend, clone B-D48).

Gurrea-Rubio M., Wu Q., Amin M.A., Tsou P.S., Campbell P.L., Amarista C.E., Ikari Y., Brodie W.D., Mattichak M.N., Muraoka S., Randon P.M., Lind M.E., Ruth J.H., Mao-Draayer Y., Ding S., Shen X., Cooney L.A., Lin F, & Fox D.A. (2023). Activation of Cytotoxic Lymphocytes Through CD6 Enhances Killing of Cancer Cells. Research Square.

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Monoclonal Antibody Characterization Assay

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UMCD6, a mouse anti-human monoclonal antibody that targets domain 1 of CD6, was created in our laboratory and was purified from mouse ascites and desalted by column chromatography using Protein G and dextran per manufacturer’s instructions (Thermo Fisher Scientific). Pembrolizumab and nivolumab (anti-PD-1) were obtained from Merck and Bristol-Myers Squibb. The following antibodies were used for flow cytometry analyses: FITC anti-human CD4 (BioLegend Cat#391503), PE anti-human CD45 (Biolegend, clone HI30), APC anti-human CD56 (Biolegend, clone 5.1H11), APC/Cyanine7 anti-human CD8a (Biolegend, San Diego, CA, USA, clone RPA-T8), PE/Cyanine7 anti-human CD3 (Biolegend, clone HIT3a), PerCP/Cyanine5.5 anti-human CD314 (NKG2D) (Biolegend, clone 1D11), FITC anti-human CD6 (Biolegend, clone BL-CD6), APC anti-human CD16 (Biolegend, clone 3G8), Pacific Blue anti-human/mouse Ki67 (Biolegend, clone 16A8), FITC anti-human/mouse Granzyme B (Biolegend, clone GB11) and APC anti-human Perforin (Biolegend, clone B-D48).

Gurrea-Rubio M., Wu Q., Amin M.A., Tsou P.S., Campbell P.L., Amarista C.I., Ikari Y., Brodie W.D., Mattichak M.N., Muraoka S., Randon P.M., Lind M.E., Ruth J.H., Mao-Draayer Y., Ding S., Shen X., Cooney L.A., Lin F, & Fox D.A. (2024). Activation of cytotoxic lymphocytes through CD6 enhances killing of cancer cells. Cancer Immunology, Immunotherapy : CII, 73(2), 34.

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