Boric acid granule was purchased from Baker Chemical Company (Phillipsburg, NJ, USA). Cells and cell culture related products were purchased from Invitrogen (Carlsbad, CA, USA). Nucleopore polycarbonate membranes (50 nm in size) were purchased from Whatman Company. Camptothecin powders (purity > 99%), Polyvinyl alcohol (PVA), Alizarin Red S(ARS), Albumin, bovine serum (BSA) and all other chemicals were purchased from Sigma-Aldrich Company (St. Louis, MO, USA). Tetra-Oacetyl-2-acetamido-2-deoxy-beta-D-mannose (Ac4ManNAc) was a gift from New Zealand Pharmaceuticals Ltd. (Palmerston North, New Zealand).
Bovine serum bsa
Manufactured by Merck Group
Sourced in United States
Bovine serum (BSA) is a commonly used laboratory reagent derived from bovine blood. It is a complex mixture of proteins, including albumin, globulins, and other components. BSA is often used as a protein source, stabilizer, and blocking agent in various biological and biochemical applications, such as cell culture, immunoassays, and protein purification.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Ssea 4, by Merck Group (1 mentions)
Cxcr4, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Protease and phosphatase inhibitor, by Merck Group (1 mentions)
Sds polyacrylamide gel, by Bio-Rad (1 mentions)
Chemidoc detector, by Bio-Rad (1 mentions)
Bca method, by Merck Group (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Clarity western ecl substrate, by Bio-Rad (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Hyclone, by Thermo Fisher Scientific (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Albumin from human serum hsa, by Merck Group (1 mentions)
Pepsin from hog stomach, by Merck Group (1 mentions)
Sodium triphosphate, by Merck Group (1 mentions)
Barnstead micropure system, by Thermo Fisher Scientific (1 mentions)
α amylase, by Merck Group (1 mentions)
Laser confocal microscope lsm 510meta, by Zeiss (1 mentions)
Lsm 510 imaging software, by Zeiss (1 mentions)
7 protocols using bovine serum bsa
1
Protocol for Synthesizing Nanoparticles
2
Immunophenotyping of Human Bone Marrow-Derived Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hBM-MSC seeded at a density 5 × 103 cells on 24-well plates, stained with Molday ION Rhodamine B™ and CellTracker™ Green CMFDA, or transfected with mRNA eEGFP on 2nd and 7th day of culture were washed three times with PBS and fixed by 15-min incubation in 4% paraformaldehyde (PFA; MP BIOMEDICALS) in PBS and then again washed three times in PBS. Permeabilization was accomplished by 3-min incubation in 0.05% Triton X100 (Triton®X-100 Sigma Ultra, Sigma-Aldrich) in PBS. Non-specific binding was blocked using 3% of albumin from bovine serum (BSA; Sigma-Aldrich) for 90 min. The cells were washed with PBS, and they were incubated overnight at 4 °C with primary antibodies against CD90 (1:250, Sigma-Aldrich), CD44 (1:500, Santa Cruz), SSEA4 (1:100, Millipore), and CXCR4 (1:250, Santa Cruz). Then, cells were washed three times with PBS, and they were exposed for 90 min (RT in the dark) to goat anti-mouse IgG (H+L)-488 (Invitrogen) or -546 (Invitrogen) secondary antibodies. Additionally, cell nuclei were stained with 5 μM Hoechst 33258 (Sigma-Aldrich). The images were captured using fluorescent microscope Axiovert.A1 Zeiss (Carl Zeiss MicroImaging GmbH). Following acquisition, images were processed using the Zeiss Axiovert.A1 software package v. ZEN blue software (Carl Zeiss MicroImaging GmbH).
3
Adhesion Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We added 50 μl of FN (20 mg/L) into each well of 96-well plates, dried overnight and kept at 4°C. We added 100 μl of 1% albumin from bovine serum (BSA) (Sigma Corporation, USA) in each well for sealing the binding site. The cells were digested and added into each well at 1, 3, 5, and 7 days after CO2 treated. Non-adhered cells were washed off by PBS after 90 min of incubation at 5% CO2 at 37°C, and the adhered cells were measured by the value of A450 nm of MTT assay. The experimental procedures were repeated 5 times.
4
Western Blot Analysis of Protein Lysates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in RIPA buffer supplemented with freshly added protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO, USA). Twenty micrograms of protein evaluated using the BCA method (Sigma-Aldrich, St. Louis, MO, USA) was loaded onto a 12% SDS polyacrylamide gel (Bio-Rad, Hercules, CA, USA) followed by electrophoresis at a constant voltage of 100 V for 1.5 h. Proteins were transferred to a PVDF membrane (0.2 μm pore size, Merck Millipore, Burlington, MA, USA). The membranes were incubated for 40 min in blocking solution: 4% bovine serum (BSA; Sigma-Aldrich, St. Louis, MO, USA) in TBS-T buffer (25 mM Tris, 0.2 M glycine, 20% methanol, 1% Tween) and incubated overnight at 4 °C with primary and then with secondary antibodies (Supplementary Materials Table S2 ). The detection was performed using a Clarity Western ECL substrate (Bio-Rad, Hercules, CA, USA) with the ChemiDoc detector (Bio-Rad, Hercules, CA, USA). Protein band intensities were quantified using ImageLab 5.2.1 software.
5
Inducing FFA Overload in HepG2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell line HepG2 was obtained from American Type Culture Collection, and cultured in Dulbecco’s minimal essential medium (DMEM) supplemented with 10% of fetal bovine serum, 100 unit/mL penicillin, 100 μg/mL streptomycin, and 2 mM glutamine (HyClone, Thermo Scientific) at 37°C, in a humidified atmosphere of 95% air-5% CO2. To induce FFAs-overloading, cells at 70% confluency were washed with PBS, and then were exposed to a long-chain mixture of FFAs (mixture of oleic acid and palmitic acid, OAPA; with the ratio of 2:1). 10 mM FFAs stock solution was prepared in 0.1 M NaOH by heating to 70°C in a shaking water bath. Next, a 10% FFA-free bovine serum BSA (Sigma Aldrich) solution was prepared in H2O, and vortex mixed with FFAs stock solution for 10 sec followed by a further 10 min incubation at 55°C. The FFAs-BSA conjugate stock solution was cooled to room temperature and sterile filtered through a 0.45 μm pore membrane filter. It can be stable stored at -20°C for 3–4 weeks, where the stock solutions were heated for 15 min at 55°C, then cooled to room temperature before use.
6
Synthesis and Evaluation of Hexahydrocurcumin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (CS, 75 kDa, 84.3 ± 0.2% deacetylated) was provided by Marine Bio-Resources Co., Ltd. (Samut Sakhon, Thailand). Sodium triphosphate (TPP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Pluronic® F-127mucin powder from porcine stomach Type III, α-amylase, porcine lipase Type II and bile extract powder, pepsin from hog stomach, pancreatin from hog pancreas, and albumin from human serum (HSA) and bovine serum (BSA) were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Life Science Production (LPS, Bedford, UK). Serum-free Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen Cop. (Grand Island, NY, USA). Hexahydrocurcumin (HHC) was synthesized from curcumin (Cur) following the method described by Srimuangwong et al. [15 (link)]. Wright-Giemsa dye was purchased from M&P IMPEX Ltd. (Ladkrabang, BKK, Thailand). Analytical grades of acetic acid, acetone, absolute ethanol, hydrogen peroxide, and dimethyl sulfoxide (DMSO) were purchased from Carlo Erba reagents (Val de Reuil, France). Ultrapure water was obtained from the Thermo Scientific Barnstead MicroPure system (Thermo Fisher Scientific Inc., Waltham, MA, USA). Other reagents were used without further purification.
7
Visualizing Osteocalcin in MG 63 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intracellular and secreted osteocalcin was visualized in MG 63 cells as described previously [16 (link)]. Briefly, the discs plated with cells were rinsed with PBS, permeabilized in 0.3% (v/v) in Triton X-100 for 90 seconds and fixed in chilled methanol at −20 °C for 10 min. To measure the formation of ECM and cell growth, non-specific sites were blocked with 5% bovine serum (BSA, Sigma), then incubated for 1 h at 37 °C with the mouse monoclonal antibody against human Osteocalcin (dilution 1:100, RnD Biosciences) as primary antibody and secondary antibody tagged with fluorescein isothiocyanate (FITC) (Sigma, MO). Images of the resultant fluorescence were then collected on Laser Confocal Microscope LSM 510META (Carl Zeiss, Germany). The images were analysed and compared using LSM 510 Imaging software, Carl Zeiss, Germany.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!