Hydroethidine dhe

Uric acid, oxonic acid, ADMA, Griess reagent, N‐acetylcysteine (NAC), apocynin (APO), and mouse antibody for α-tubulin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rabbit antibodies for DDAH-1, DDAH-2, protein arginine methyltransferases 1 (PRMT1), and cationic amino acid transporter-1 (CAT-1) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit antibodies for intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were purchased from Cell Signaling Technology (Beverly, MA, USA). Our ADMA ELISA kit was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Our EnzyChrom NADP⁺/NADPH assay kit (ECNP-100) was obtained from BioAssay Systems (Hayward, CA, USA). Hydroethidine (DHE) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Molecular Probes (Eugene, OR, USA). Our ELISA kits for tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, monocyte chemoattractant protein 1 (MCP-1), and macrophage inflammatory protein 2 (MIP-2) were obtained from R&D Systems (Minneapolis, MN, USA).

Simvastatin, ADMA, SDMA, mouse antibody for α‐tubulin, and Griess reagent were from Sigma‐Aldrich (St Louis, MO). Rabbit antibody for phospho‐eNOS Ser1179 was from Cell Signaling Technology (Beverly, MA). Rabbit antibodies for eNOS and DDAH‐2 were from Santa Cruz Biotechnology (Santa Cruz, CA). The cGMP enzyme immunoassay kit was from R&D Systems (Minneapolis, MN). ECL Cell Attachment Matrix was from Millipore (Bedford, MA). The EnzyChrom NADP⁺/NAD(P)H assay kit was from BioAssay Systems (Hayward, CA). Hydroethidine (DHE) and 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA) were from Molecular Probes (Eugene, OR).