Ifn γ fitc clone 4 s b3

Manufactured by BioLegend

IFN-γ FITC (clone 4 S.B3) is a fluorescently labeled antibody used for the detection and analysis of interferon-gamma (IFN-γ) in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Golgi stop and golgi plug, by BD (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Perm wash buffer, by BD (1 mentions) Cd20 pe cy7 clone 2h7, by BioLegend (1 mentions) Cd4 apc h7 clone l200, by BD (1 mentions) Cd3 pe clone sp34 2, by BD (1 mentions) Anti cd49d clone 9f10, by Thermo Fisher Scientific (1 mentions) Cd56 pe, by Beckman Coulter (1 mentions) Recombinant human il 12, by R&D Systems (1 mentions) Fixable viability dye ef780, by Thermo Fisher Scientific (1 mentions) Recombinant human il 18, by R&D Systems (1 mentions)

Close spelling variants of this product

IFN-γ (422 citations) IFN-γ-FITC (23 citations)

2 protocols using ifn γ fitc clone 4 s b3

TRAP-stimulated CD4+ and CD8+ T cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen PBMC samples were thawed and rested for 6 hours at 37 °C in media containing 10U/ml of Benzonase (Novagen). Cells were then stimulated for a total of 16 hours with a single pool of TRAP peptides at 2 μg/ml final concentration, anti-CD28 APC (clone CD28.2, eBioscience), anti-CD49d (clone 9F10, eBioscience), anti-CD107a PECy5 (clone eBioH4.A3, eBioscience), Golgi-Stop and Golgi-Plug (BD Bioscience). Surface staining with CD95-bi (clone DX2, eBioscience) av-qDot565 (Invitrogen), CD4-APC H7 (clone L200, BD Bioscience), CD14 PE-Cy7 (clone M5E2, BioLegend), CD20 PE-Cy7 (clone 2H7, BioLegend) and live-dead Aqua was followed by fixation in 10% neutral buffered formalin (Sigma), prior to intracellular staining for CD3-PE (clone SP34–2, BD Bioscience), CD8 APC-H7 (clone RPA-T8, eBioscience) and IFN-γ FITC (clone 4 S.B3, BioLegend) in Perm-Wash Buffer (BD). All flow cytometry data were analysed using FlowJo (TreeStar) with cells gated based on size, doublet negative, CD3⁺ CD14/CD20⁻, and either CD4⁺CD8⁻ or CD4⁻CD8⁺.

Rollier C.S., Spencer A.J., Sogaard K.C., Honeycutt J., Furze J., Bregu M., Gilbert S.C., Wyllie D, & Hill A.V. (2020). Modification of Adenovirus vaccine vector-induced immune responses by expression of a signalling molecule. Scientific Reports, 10, 5716.

+ Open protocol

+ Expand

PBMC Cytotoxicity Assay with IL-12/IL-18 Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs from patients (n=69) and healthy controls (n = 4) were cocultured with K562 target cells at a 2:1 ratio for 4 hours in the presence of CD107a. Golgi Plug/Stop was added after 1 hour ^{26 (link)}. Intracellular cytokine-production was measured by IFN-γ and TNF-α. The following antibodies were used: CD107a-AF700 (clone H4A3, BD), Fixable Viability dye eF780 (eBioscience), CD56-PE (Beckman Coulter), the same antibodies as above for CD3, CD14, CD19, IFN-γ-FITC (clone 4S.B3, Biolegend), and TNFα-AF647 (clone Mab11, Biolegend). Recombinant human IL-12 (10ng/mL) and recombinant human IL-18 (100 ng/mL), both from R&D, were added to one well of PBMCs and incubated overnight for maximal cytokine stimulation. Where stated, patient samples were cultured in media containing the IL-15 superagonist ALT803 (1 nM) overnight prior to degranulation assays.

Bergerson R.J., Williams R., Wang H., Shanley R., Colbenson G., Kerber A., Cooley S., Curtsinger J.M., Felices M., Miller J.S, & Verneris M.R. (2016). Fewer circulating natural killer cells 28 days after double cord blood transplantation predicts inferior survival and IL-15 response. Blood Advances, 1(3), 208-218.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!