Somatostatin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Somatostatin is a peptide hormone that inhibits the release of other hormones, such as growth hormone, thyroid-stimulating hormone, and insulin. It is commonly used in research and clinical applications involving the regulation of hormone secretion.

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Lab products found in correlation

Insulin, by Thermo Fisher Scientific (2 mentions) Transferrin, by Thermo Fisher Scientific (1 mentions) Hydrocortisone, by Thermo Fisher Scientific (1 mentions) Bovine serum, by Thermo Fisher Scientific (1 mentions) Bovine tsh, by Thermo Fisher Scientific (1 mentions) Glucagon, by Thermo Fisher Scientific (1 mentions)

2 protocols using somatostatin

FRTL-5 Cell Culture with PV Treatments

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FRTL-5 rat thyroid cells were grown in Coon’s modified Ham’s F-12 medium containing 5% heat-treated bovine serum (Invitrogen, Carlsbad, CA) and a mixture of six hormones, including bovine TSH (1 mU/mL), insulin (10 μg/mL), hydrocortisone (0.36 ng/mL), transferrin (5 μg/mL), Gly-His-Lys-acetate (2 ng/mL), and somatostatin (10 ng/mL) as described (20 (link)). All reagents were purchased from Sigma-Aldrich (St. Louis, MO). PV was used at concentrations of 0, 31.25, 62.5, 125, 250, 500 μg/mL in culture medium.

Chen F., Kawashima A., Luo Y., Kiriya M, & Suzuki K. (2020). Innate Immune-Modulatory Activity of Prunella vulgaris in Thyrocytes Functions as a Potential Mechanism for Treating Hashimoto’s Thyroiditis. Frontiers in Endocrinology, 11, 579648.

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Immunostaining of NPY-pHluorin in Human Islets

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Human islets infected for 5 days with NPY-pHluorin were fixed with 4% (vol./vol.) paraformaldehyde for 30–60 min on a microscope slide and immunostained for insulin, glucagon or somatostatin and GFP (Invitrogen, Carlsbad, CA, USA) using protocols previously described [15 (link)]. To assess co-localisation of NPY–pHluorin with islet hormones, we used ImageJ co-localisation analysis plugin ‘Intensity Correlation Analysis’ and calculated Mander’s overlap coefficients (R). R values over 0.6 denote co-localisation [19 (link)].

Almaça J., Liang T., Gaisano H.Y., Nam H.G., Berggren P.O, & Caicedo A. (2015). Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets. Diabetologia, 58(12), 2810-2818.

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