Mouse anti rat cd68 ed1

The reagents were as follows: fibronectin (FN) (Sigma; Rehovot, Israel) VCAM-1(R&D Biosystems, Minneapolis, MN, USA); BSA (Sigma); XTT cell proliferation kit (Biological Industries, Bet Haemek, Israel); FAM FLICA Caspase-3,7 and caspase-1 detection kit (Immunochemistry Technologies LLC, MN, USA); anti Cd49d, anti cd49e (Serotec, NC, USA); Staphylococcus aureus Cowan I (SAC) (Calbiochem-Behring Corp., La Jolla, CA, USA). LPS (Sigma); mouse anti-rat CD68 (ED1) (Serotec); rabbit anti-rat FN (Cedarlane, Burlington, ON, Canada); anti-mouse IL-18 (R&D Biosystems); anti-mouse IL-1β (Santa Cruz, Santa Cruz, CA, USA); anti αTubulin (Santa Cruz); anti-rat Thy-1.1(Cederlane); sheep anti-rat GBM (Probetex, San Antonio, TX, USA); rat IL-1β, IL-18, and TNFα ELISA kits (BiosourceThermo Fisher, Waltham, MA, USA); rat connective tissue growth factor (CTGF) and monocyte chemoattractant protein-1 (MCP-1) ELISA kits (MyBiosource, San Diego CA, USA). Creatinine and albumin assay kits (Abcam, San Francisco, CA, USA); AS101 (supplied by M. Albeck, Bar-Ilan University, Ramat Gan, Israel).

Diverse primary antibodies were used: rabbit anti-Iba1 (IgG; 1:100), a marker of quiescent and active microglia (Waco Chemicals, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP) (IgG2b; 1:100) (BD Pharmingen Franklin Lakes, NJ, USA), mouse anti-neuN (IgG1; 1:100), mouse anti-rat CD68/ED1 (IgG1; 1:100), a marker of activated microglial cells in a phagocytic state (Serotec, Oxford, UK). Secondary antibodies were used at 1:100 dilution: FITC and Tetramethyl Rhodamine IsoThioCyanate (TRITC)-conjugated goat anti-mouse IgG (γ-chain specific and against whole molecule respectively), TRITC-conjugated goat anti-rabbit IgG (whole molecule), FITC-conjugated sheep anti-rabbit IgG (whole molecule) (Sigma, Saint-Louis, MO, USA), FITC and TRITC-conjugated donkey anti-mouse IgG (whole molecule), FITC and TRITC-conjugated donkey anti-sheep IgG (whole molecule), Cy3-conjugated donkey anti-rabbit IgG (whole molecule) and anti-goat IgG (whole molecule) (Jackson ImmunoResearch Laboratories, Inc., WestGrove, PA, USA).