Secondary antimouse igg hrp conjugated antibody

Manufactured by Cell Signaling Technology

The Secondary antimouse IgG-HRP conjugated antibody is a detection reagent used in immunoassays. It binds to mouse immunoglobulin G (IgG) and is conjugated to horseradish peroxidase (HRP), which can catalyze a color-producing reaction for signal detection.

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Lab products found in correlation

Model 680 microplate reader, by Bio-Rad (2 mentions) 3 3 5 5 tetramethylbenzidine tmb substrate, by BioLegend (1 mentions) Tmb substrate, by BioLegend (1 mentions) 96 well plate, by Corning (1 mentions)

Close spelling variants of this product

HRP-conjugated secondary antibodies (725 citations)

2 protocols using secondary antimouse igg hrp conjugated antibody

HER2-expressing Cell Binding Assay

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Serum from vaccinated mice was diluted and added to parental or human HER2-expressing NMUMG cells for 1 hour at room temperature. Plates were washed and cells were fixed with 1% formalin. A secondary antimouse IgG-HRP conjugated antibody (Cell Signaling Technology) was used prior to developing with 3,3',5,5'-Tetramethylbenzidine (TMB) substrate (Biolegend) and absorbance determined using a Bio-Rad Model 680 microplate reader (Bio-Rad).

Chen A.C., Xu R., Wang T., Wei J., Yang X.Y., Liu C.X., Lei G., Lyerly H.K., Heiland T, & Hartman Z.C. (2020). HER2-LAMP vaccines effectively traffic to endolysosomal compartments and generate enhanced polyfunctional T cell responses that induce complete tumor regression. Journal for Immunotherapy of Cancer, 8(1), e000258.

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Quantifying Cellular Signaling Responses

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Parental or hHER2-expressing NMUMG cells were plated overnight in 96-well plates (Corning). Serum was diluted and added to plates for 1 hour. Plates were washed and cells were fixed with 1% formalin. A secondary anti-mouse IgG-HRP conjugated antibody (Cell Signaling) was used prior to developing with TMB substrate (Biolegend) and absorbance determined using a Bio-Rad Model 680 microplate reader (Bio-Rad).

Crosby E.J., Gwin W., Blackwell K., Marcom P.K., Chang S., Maecker H.T., Broadwater G., Hyslop T., Kim S., Rogatko A., Lubkov V., Snyder J.C., Osada T., Hobeika A.C., Morse M.A., Lyerly H.K, & Hartman Z.C. (2019). Vaccine-induced memory CD8+ T cells provide clinical benefit in HER2 expressing breast cancer: a mouse to human translational study. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(9), 2725-2736.

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