AAPH (2,2′-azobis (2-amidinopropane)) (Acros Organics), sodium phosphate dibasic, sodium chloride, potassium dihydrogen phosphate, potassium sulphate, TRIS (ultrapure), glycine (proteomics grade), ortho-boric acid, and methanol (HPLC grade) were obtained from VWR International Eurolab S.L. (Barcelona, Spain). Trizma® base, ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonic acid)), DTT (DL-Dithiothreitol), Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), fluorescein sodium salt, formic acid (reagent grade ≥ 95%), and diatomaceous earth (Hyflo® Super Cel®) were provided by Sigma-Aldrich (Steinheim, Germany). Sodium hydroxide, glacial acetic acid, and sulfuric acid were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate) and nitric acid (65% p/p) were purchased from Panreac (Barcelona, Spain). Bromophenol blue indicator (ACS reagent), acetonitrile (HPLC grade), trifluoroacetic acid, acetone, and glycerol were provided by Merck (Darmstadt, Germany). Absolute ethanol was obtained from J.T. Baker (Deventer, The Netherlands), Octadecyl C18 sorbent was obtained from Phenomenex (Madrid, Spain), and anhydrous magnesium sulfate (99.5% min powder) was obtained from Alfa Aesar (Karlsruhe, Germany). Deionized water with a resistivity of >18 MΩ/cm was obtained through a Milli-Q SP® Reagent Water System (Millipore Corporation Bedford, MA, USA).
Octadecyl c18 sorbent
Manufactured by Phenomenex
Sourced in Spain
The Octadecyl C18 sorbent is a reversed-phase liquid chromatography material. It consists of silica particles with covalently bonded octadecyl (C18) functional groups. This sorbent is commonly used for the separation and purification of a wide range of organic compounds in various analytical and preparative applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Thermo Fisher Scientific (3 mentions)
Glycerol, by Merck Group (3 mentions)
Sodium chloride (nacl), by Avantor (3 mentions)
Sulfuric acid, by Thermo Fisher Scientific (3 mentions)
Trolox, by Merck Group (3 mentions)
Milli q sp reagent water system, by Merck Group (3 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (3 mentions)
Hyflo super cel, by Merck Group (3 mentions)
Formic acid, by Merck Group (3 mentions)
Anhydrous magnesium sulfate, by Thermo Fisher Scientific (2 mentions)
Fluorescein sodium salt, by Merck Group (2 mentions)
Methanol, by Avantor (2 mentions)
Acetone, by Merck Group (2 mentions)
Trizma base, by Merck Group (2 mentions)
Potassium dihydrogen phosphate, by Avantor (2 mentions)
Aaph 2 2 azobis 2 amidinopropane, by Thermo Fisher Scientific (2 mentions)
Bromophenol blue indicator acs reagent, by Merck Group (2 mentions)
Nitric acid, by ITW Reagents (2 mentions)
Sodium phosphate dibasic, by Thermo Fisher Scientific (2 mentions)
Absolute ethanol, by Phenomenex (2 mentions)
4 protocols using octadecyl c18 sorbent
1
Comprehensive Reagents for Analytical Techniques
2
Antioxidant Assay Reagents Protocol
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Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonic acid)), DTT (DL-Dithiothreitol), Trizma® base, fluorescein sodium salt, diatomaceous earth (Hyflo® Super Cel®), and formic acid (reagent grade ≥ 95%) were provided by Sigma-Aldrich (Steinheim, Germany). Potassium dihydrogen phosphate, sodium phosphate dibasic, potassium sulfate, sodium chloride, ortho-boric acid, TRIS (ultrapure), AAPH (2,2′-azobis (2-amidinopropane)) (Acros Organics), glycine (proteomics grade), and methanol (HPLC LC-MS grade) were purchased from VWR International Eurolab S.L. (Barcelona, Spain). Sodium hydroxide, glacial acetic acid, and sulfuric acid were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate, purissimum-CODEX) was obtained from Panreac (Barcelona, Spain). Acetonitrile (HPLC grade), acetone, glycerol, and bromophenol blue indicator (ACS reagent) were supplied by Merck (Darmstadt, Germany). Anhydrous magnesium sulfate (99.5% min powder) was provided by Alfa Aesar (Karlsruhe, Germany). Octadecyl C18 sorbent was from Phenomenex (Madrid, Spain) while absolute ethanol was from J.T. Baker (Deventer, The Netherlands). Deionized water (resistivity >18 MΩ cm−1) was obtained through a Milli-Q SP® Reagent Water System (Millipore Corporation, Bedford, MA, USA).
3
Antioxidant Capacity Quantification Assays
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ABTS (2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid)), Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DTT (dl -dithiothreitol), diatomaceous earth (Hyflo® Super Cel®), Trizma® base, fluorescein sodium salt, and formic acid (reagent grade ≥ 95%) were purchased from Sigma-Aldrich (Steinheim, Germany). AAPH (2,2′-azobis(2-amidinopropane)) (Acros Organics), Tris (ultrapure), potassium dihydrogen phosphate, potassium sulfate, sodium phosphate dibasic, sodium chloride, ortho-boric acid, glycine (proteomics grade), and methanol (HPLC–MS grade) were provided by VWR International Eurolab S.L. (Barcelona, Spain). Glacial acetic acid, sulfuric acid, and sodium hydroxide were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate, purissimum-CODEX) and nitric acid (65% p/p) were obtained from Panreac (Barcelona, Spain). Acetonitrile (HPLC grade), acetone, glycerol, and bromophenol blue indicator (ACS reagent) were supplied by Merck (Darmstadt, Germany). Octadecyl C18 sorbent was from Phenomenex (Madrid, Spain), while absolute ethanol was from J.T. Baker (Deventer, The Netherlands). Anhydrous magnesium sulfate (99.5% min powder) was provided by Alfa Aesar (Karlsruhe, Germany). Deionized water (resistivity >18 MΩ/cm) was obtained through a Milli-Q SP® Reagent Water System (Millipore Corporation Bedford, Bedford, MA, USA).
4
Mycotoxin Analysis by HPLC-MS/MS
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Acetonitrile (ACN) and methanol (MeOH) HPLC grade were purchased from Merck (Darmstadt, Germany). Deionized water (resistivity > 18 MΩ cm−1) was obtained using a Milli-Q SP® Reagent Water System (Millipore Corporation Bedford, USA). Ammonium formate (99%) was supplied by Panreac Quimica S.A.U. (Barcelona, Spain) and formic acid (reagent grade ≥ 95%) was supplied by Sigma-Aldrich (St. Louis, MO, USA). All solvents were filtered through a 0.45 μm cellulose filter supplied by Scharlau (Barcelona, Spain) before use. Salts for QuEChERS extraction: sodium chloride (NaCl) was obtained from VWR Chemicals (Leuven, Belgium), Magnesium sulfate (MgSO4), anhydrous 99.5% min powder was supplied by Alfa Aesar (Karlsruhe, Germany) and Octadecyl C18 sorbent was acquired from Phenomenex (Madrid, Spain). Before injection, samples were filtered through a nylon filter (13 mm/0.22 μm) from Membrane Solutions (TX, USA). Mycotoxins standards (AFB1, AFB2, AFG1, AFG2, ZEA, OTA, ENNA, ENNA1, ENNB, ENNB1 and BEA) were supplied by Sigma (St. Louis, MO, USA). Individual stock solutions of each mycotoxin were prepared in MeOH at concentration of 100 mg/l. The working solutions were prepared from these individual stock solutions. All prepared solutions were stored in darkness at − 20 °C until the analysis.
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