The MtbΔwhiB4 cells overexpressing either FLAG-tagged WhiB4 (whiB4-OE strain) or histidine-tagged WhiB4 (His-WhiB4) were fixed with 4% PFA, washed with 1X PBS and permeabilized using 0.1% of Triton-X 100. Cells were blocked with 2% bovine serum albumin (BSA) and incubated with anti-FLAG or anti-His primary antibody. The cells were incubated with anti-mouse IgG Alexa Fluor® 594-conjugated secondary antibody (Invitrogen) followed by staining with DAPI as described earlier. A Leica TCS Sp5 confocal microscope under a 63X oil immersion objective was used for imaging.
Anti mouse igg alexa fluor 594 conjugated secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-mouse IgG Alexa Fluor® 594-conjugated secondary antibody is a fluorescently-labeled reagent used for the detection and visualization of mouse immunoglobulin G (IgG) in various immunoassay techniques. The antibody is conjugated with the Alexa Fluor® 594 fluorescent dye, which emits in the red region of the visible spectrum.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti mouse igg alexa fluor 594 conjugated secondary antibody
1
Visualization of WhiB4 Localization in Mtb
2
Synaptogenesis Modulation by Brain Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neurons on 1 DIV were co-cultured with BMEC or treated with B-CM. After 2 days, neurons were fixed and stained with mouse monoclonal antibody MAP2 (1:200; catalog M4403; Sigma-Aldrich) and then incubated with anti-mouse IgG Alexa Fluor 594-conjugated secondary antibody (1:1000; catalog 21203; Invitrogen, Carlsbad, CA, USA).
To test the effects of BMEC co-culture or B-CM on synaptogenesis, neurons on 12 DIV were co-cultured with BMEC or treated with B-CM for 2 days. On 14 DIV, neurons were fixed and stained for presynaptic and postsynaptic markers VGlut-1 (rabbit anti-VGlut-1, 1:200; catalog 48-2400; Invitrogen) and PSD95 (mouse anti-PSD95, 1:200; catalog MAB1596; Millipore). Alexa-conjugated secondary antibodies (anti-rabbit IgG Alexa Fluor 594, 1:1000; catalog A21207 and anti-mouse IgG Alexa Fluor 488, 1:1000; catalog A21202) were used for detection. The fluorescent images were captured using a confocal microscope (SP8, Leica, Wetzlar, Germany).
To test the effects of BMEC co-culture or B-CM on synaptogenesis, neurons on 12 DIV were co-cultured with BMEC or treated with B-CM for 2 days. On 14 DIV, neurons were fixed and stained for presynaptic and postsynaptic markers VGlut-1 (rabbit anti-VGlut-1, 1:200; catalog 48-2400; Invitrogen) and PSD95 (mouse anti-PSD95, 1:200; catalog MAB1596; Millipore). Alexa-conjugated secondary antibodies (anti-rabbit IgG Alexa Fluor 594, 1:1000; catalog A21207 and anti-mouse IgG Alexa Fluor 488, 1:1000; catalog A21202) were used for detection. The fluorescent images were captured using a confocal microscope (SP8, Leica, Wetzlar, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!