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Bv605 tnfα

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BV605-TNFα is a fluorochrome-conjugated antibody that binds to tumor necrosis factor alpha (TNFα), a cytokine involved in inflammation and immune response. It is designed for use in flow cytometry applications to detect and quantify TNFα-expressing cells.

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Lab products found in correlation

Granzyme b pacific blue, by BioLegend (1 mentions) Rabbit anti laminin, by Merck Group (1 mentions) G418 sulfate, by Thermo Fisher Scientific (1 mentions) Cd4 apc cy7, by BioLegend (1 mentions) Pe gr1, by BioLegend (1 mentions) Percp cy5.5 cd45, by BioLegend (1 mentions) Foxp3 pe, by BioLegend (1 mentions) Apc ifnγ, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem hepes, by Thermo Fisher Scientific (1 mentions) Rabbit antibody against β actin, by Cell Signaling Technology (1 mentions) Rabbit anti ionized calcium binding adapter molecule 1 iba1, by Fujifilm (1 mentions) Bv421 tgfβ, by BioLegend (1 mentions) Pe cy7 il10, by BioLegend (1 mentions) Cd25 alexa fluor 700, by BioLegend (1 mentions) Pd 1 bv605, by BioLegend (1 mentions) Apc nk1, by BioLegend (1 mentions) Percp cy5.5 cd8a, by BioLegend (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rat anti mouse cd31, by BD (1 mentions)

Close spelling variants of this product

TNFα-bv605 (Mab11) Anti-TNFα BV605 Anti-mouse TNFα-BV605

2 protocols using bv605 tnfα

1

Molecular Analysis of Cell Signaling Pathways

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Dulbecco’s Modified Eagle Medium (DMEM/HEPES, Cat #12430-054) and Penicillin/Streptomycin (Cat #15240062) were from Gibco (Carlsbad, CA). Fetal bovine serum (FBS) and G418 sulfate were obtained from Invitrogen (Carlsbad, CA). Rabbit antibody against β-actin (Cat #4970S) was from Cell Signaling (Beverly, MA). Rabbit antibody against NHE1 (Cat #ab67314) and rat antibody against CD8 (Cat #ab22378) were from Abcam Ltd. (Cambridge, MA). Rat anti-mouse CD31 (Cat #550274) was from BD Pharmingen (San Jose, CA). Rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) was from Wako (Richmond, VA). PerCP/Cy5.5-CD45, PE-P2RY12, BV421-TGFβ, BV605-TNFα, APC/780-IL-1β, PE/Cy7-IL10, PerCP/Cy5.5-CD8a, APC/Cy7-CD4, Alexa Fluor 700-CD25, PE-FoxP3, BV605-PD-1, PE-Gr-1, APC-NK1.1, and Pacific Blue Granzyme B were obtained from Biolegend (San Diego, CA). eFluor 450-CD16/32, PE/Cy7-CD206, APC-IFNγ was purchased from eBioscience (San Diego, CA). APC-IL-6 were obtained from thermos fisher (Waltham, MA). BUV737-CD11b, Alexa Flour 700-CD86 were obtained from BD Biosciences (San Jose, CA). PE-Ym1were from Abcam Ltd. (Cambridge, MA). Rabbit anti-Laminin (Cat #L9393) and cariporide (HOE642) was purchased from Sigma Chemicals (St. Louis, MO). Anti-mouse PD-1 (RMP1-14) and IgG2a isotype control were from BioXcell (West Lebanon, NH).
Guan X., Luo L., Begum G., Kohanbash G., Song Q., Rao A., Amankulor N., Sun B., Sun D, & Jia W. (2018). Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas. Journal of Experimental & Clinical Cancer Research : CR, 37, 255.
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2

Peptide Library Synthesis and Liposome Formulation

Cited 1 time
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Trp2, AH1 and WT1 peptide libraries and peptides were synthesized by GenScript. CoPoP was synthesized as previously reported (32 (link)). The following lipids were used to produce liposomes: dioleoylphosphatidylcholine (DOPC; Corden #LP-R4–070), cholesterol (PhytoChol; Wilshire), and synthetic PHAD-3D6A (Avanti; #699855). QS-21 was purchased from Desert King (part number NC0949192). The APC-CD8a (Clone: CT-CD8a) antibody was obtained from Accurate Chemical and Scientific Corporation (#ACL168APC). The following antibodies were obtained from BioLegend: APC-CD8a (Clone: CT-CD8a; #100712), FITC-CD4 (Clone: GK1.5; #100405), AF700-CD45 (Clone: 30-F11; #103127), APC-Cy7-CD44 (Clone: IM7; #103027), PE/Cy7-CD62L (Clone: MEL-14; #104417), PerCP-Cy5.5-PD-1 (Clone: RMP1–30; #109119), pacific blue-IFN-γ (Clone: XMG1.2; #505818), BV605-TNF-α (Clone: MP6-XT22; #506329), PE/Cy7-Granzyme B (Clone: QA16A02; #372213). Anti-mouse PD-1 (#BP0146) and anti-mouse CTLA-4 (#BP0131) were acquired from Bio X Cell. Other reagents used were Golgiplug/Brefeldin A (BD; #555029), Live/Dead dye (Invitrogen; #L34857), Fc-block (Clone: 2.4G2; BD #553142), fixation/permeabilization kit (BD #554714), red blood cell (RBC) lysis buffer (BioVision #5830), Collagenase Type I (Gibco #17018–029), and DNase I (Roche #04536282001).
He X., Zhou S., Quinn B., Jahagirdar D., Ortega J., Long M.D., Abrams S.I, & Lovell J.F. (2022). An In Vivo Screen to Identify Short Peptide Mimotopes with Enhanced Antitumor Immunogenicity. Cancer immunology research, 10(3), 314-326.
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