Proteins were extracted from renal tissue using an ice-cold lysis buffer, and the protein concentrations were quantified using a BCA assay kit (PC0020; Solarbio). SDS-PAGE was used to separate equal amounts of protein. The blocked membrane was incubated using anti-Bax (1 : 500; WL01637, WanleiBio), anti-P-p53 (1 : 2,000; ab1431, Abcam), anti-PUMA (1 : 3,000; ab9643, Abcam), anti-caspase-3 (1 : 2,000; 9661, CST), anti-Nrf2 (1 : 500; ab89443, Abcam), anti-HO-1 (1 : 500; WL01637, WanleiBio), anti-OCT2 (1 : 500; ab243153, Abcam), anti-MRP2 (1 : 500; ab203397, Abcam), and anti-β-actin (1 : 1,000; WL01372, WanleiBio) antibodies overnight at 4°C. Then, the membranes were incubated with a suitable horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature. Finally, an ECL western blotting substrate was used to visualize the immunoblots.
Ab243153
Manufactured by Abcam
Ab243153 is a primary antibody that recognizes a specific target. It is intended for use in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Ab203397, by Abcam (1 mentions)
Ab89443, by Abcam (1 mentions)
Ab1431, by Abcam (1 mentions)
Ab9643, by Abcam (1 mentions)
Wl01637, by Wanlei (1 mentions)
Wl01372, by Wanlei (1 mentions)
Pc0020, by Solarbio (1 mentions)
Ab184305, by Abcam (1 mentions)
Ab22819, by Abcam (1 mentions)
Ab28771, by Abcam (1 mentions)
Gel doc xr, by Bio-Rad (1 mentions)
Anti β actin, by Beyotime (1 mentions)
Anti α tubulin, by Beyotime (1 mentions)
Anti cd81, by Proteintech (1 mentions)
Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Beyotime (1 mentions)
Anti tsg101, by Abcam (1 mentions)
2 protocols using ab243153
1
Renal Protein Extraction and Western Blot
2
Protein Extraction and Characterization from Cells and Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RIPA lysis buffer (Thermo Fisher) was used to extract proteins from cells and tissues. Serum protein extraction kit (China Qincheng Biological) to extract serum protein. The immune complexes were detected with ECL Western blotting substrate (Thermo Fisher) and developed with BIO-RAD (BIO-RAD Gel Doc XR+). Western blot analysis was performed using standard procedures. The following antibodies (11000) were used: anti-β-actin (Beyotime, AF0003); anti-α-tubulin (Beyotime, AF0001); anti-GAPDH (Beyotime, AF0006); anti-PUF60 (ab22819, Abcam). Anti-IGF2BP1 (ab184305, Abcam); anti-POU2F2 (ab243153, Abcam); anti-HOXA3(ab28771, Abcam). anti-Calnexin (Abcam, ab92573); anti-CD63 (Abcam, ab134045); anti-TSG101 (Abcam, ab125011); anti-CD81 (Proteintech, 66,866-1-Ig).
Perform IHC using standard procedures. Anti-IGF2BP1, POU2F2 and HOXA3 antibodies were used for IHC(Abcam,1: 200). Images were scanned by Pannorama Scan(3DHistech, Hungary). IP was performed with anti-PUF60 antibody (AbCAM, 1:150) and appropriate control IgG(Merck micropore).
Immunoprecipitates were collected by centrifugation and analyzed by SDS-PAGE.
Perform IHC using standard procedures. Anti-IGF2BP1, POU2F2 and HOXA3 antibodies were used for IHC(Abcam,1: 200). Images were scanned by Pannorama Scan(3DHistech, Hungary). IP was performed with anti-PUF60 antibody (AbCAM, 1:150) and appropriate control IgG(Merck micropore).
Immunoprecipitates were collected by centrifugation and analyzed by SDS-PAGE.
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