Xyloglucan

The restriction endonucleases, T4 DNA ligase and endo-β-N-acetylglucosaminidase H (Endo H) were purchased from New England Biolabs (Ipswich, MA). The Taq DNA polymerase was from TaKaRa (Dalian, China). The DNA isolation and purification kits were purchased from Tiangen (Beijing, China) and Omega (Cowpens, SC), respectively. RNeasy plant mini kit and ReverTra-α-^™ kit were supplied by Qiagen (QIAGEN, Germany) and TOYOBO (Osaka, Japan), respectively. The substrates lichenan, barley β-glucan, laminarin, Avicel, carboxymethyl cellulose sodium (CMC-Na), birchwood xylan, xyloglucan, konjac glucomannan, locus bean gum, 4-nitrophenyl β-d-cellobioside (pNPC), p-nitrophenyl-β-d-glucopyranoside (pNPGlu), and 4-nitrophenyl-α-d- galactopyranoside (pNPGal) were purchased from Sigma-Aldrich (St. Louis, MO). PASC treated by 85% of phosphoric acid and SECS were supplied by the Institute of Process, Chinese Academy of Sciences. And all other chemicals were of analytical grade and commercially available.

Chemicals for cultivation of Escherichia coli and P. chrysosporium DSM 1556, including yeast extract, malt extract, soytone, lysogeny broth, TRIS, MES, and salts for minimal media were obtained from Carl Roth (Germany). Beech wood chips for growth were obtained from J. Rettenmaier Söhne GmbH & Co. KG. (Germany). Carboxymethyl cellulose (CMC), lichenan, mannan, xyloglucan, and glucomannan were purchased from Sigma Aldrich (USA), and beech wood xylan was purchased from Carl Roth (Germany). para-nitrophenol (pNP), para-nitrophenyl-β-d-galactopyranoside (pNP-Gal), para-nitrophenyl-acetate (pNP-acetate), para-nitrophenyl-β-d-glucopyranoside (pNP-Glc), para-nitrophenyl-β-d-xylopyranoside (pNP-Xyl), para-nitrophenyl-β-d-mannose (pNP-Man), para-nitrophenyl-β-d-arabinofuranoside (pNP-Ara), and para-nitrophenyl-N-acetyl-β-d-glucosamine (pNP-GlcNAc) were purchased from Megazyme (Ireland), n-dodecyl β-d-maltoside (DDM) was obtained from Thermo Scientific (USA) and bovine serum albumin (BSA) from VWR Chemicals (USA). The sources of supply of more methodology-specific reagents are reported in the corresponding procedure section.