Reagents for in vitro experiments included SR141716A (Rimonabant hydrochloride, 5 μM, Sigma), WIN55,212–2 (Tocris), GAT211 (5 μM, Sigma) were dissolved in 0.5% DMSO.
Rimonabant hydrochloride
Manufactured by Merck Group
Sourced in United States
Rimonabant hydrochloride is a synthetic cannabinoid receptor antagonist compound. It is used as a research chemical in laboratory settings to study the endocannabinoid system and related physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Win55 212 2, by Bio-Techne (1 mentions)
Gat211, by Merck Group (1 mentions)
Monensin, by Merck Group (1 mentions)
Saponin, by Avantor (1 mentions)
Sr144528, by Merck Group (1 mentions)
7 aad solution, by BioLegend (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Rimonabant, by Merck Group (1 mentions)
3 protocols using rimonabant hydrochloride
1
Cannabinoid Receptor Modulation in Vitro
2
Cannabinoid Receptor Modulation in Cell Assays
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Arachidonyl-2′-chloroethylamide hydrate (ACEA; CB1 agonist), Rimonabant hydrochloride (SR141716A; CB1 antagonist/inverse agonist), GW833972A (CB2 agonist), SR144528 (CB2 antagonist/inverse agonist), and dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA) were used. These cannabinoid receptor agonists and antagonists were dissolved in DMSO at a concentration of 20 mM and stored at −20 °C until used.
Saponin (Amresco, Solon, OH, USA) was used. Carboxyfluorescein succinimidyl ester (CFSE), brefeldin A, and monensin were obtained from Sigma-Aldrich. RPMI 1640 medium and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) were used. Ficoll-Hypaque solution (IsoPrep)(Robbins Scientific Corporation, Sunnyvale, CA, USA) was used. Finally, 7-AAD solution was purchased from BioLegend.
Saponin (Amresco, Solon, OH, USA) was used. Carboxyfluorescein succinimidyl ester (CFSE), brefeldin A, and monensin were obtained from Sigma-Aldrich. RPMI 1640 medium and fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) were used. Ficoll-Hypaque solution (IsoPrep)(Robbins Scientific Corporation, Sunnyvale, CA, USA) was used. Finally, 7-AAD solution was purchased from BioLegend.
3
Quantification of CB1 Receptor Binding in BAT
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Biopsies of intrascapular BAT (iBAT) and subcutaneous WAT from Sprague-Dawley rats were frozen to 280°C and processed into 10-mm sections. To study tracer binding properties to the tissue, the sections were incubated in concentrations in the nanomolar range (0.05-1.5 nM) of 18 F-FMPEP-d 2 in 50 mM Tris-HCl (pH 7.6) for 90 min at room temperature. Nondisplaceable binding was assessed by adding 10 mM of the CB1 antagonist rimonabant (rimonabant hydrochloride; Sigma-Aldrich) 10 min before 18 F-FMPEP-d 2 . Tissue slices were then washed 3 times for 4 min in 50 mM ice-cold Tris to remove excess tracer and then air-dried. The sections were exposed against a phosphor-imager screen (Fuji BAS-TR2025; Fuji Photo Film Co.) for 3 h, digitalized using a BAS-5000 Fuji scanner (Fujifilm Life Science), and analyzed using the AIDA 4 (Raytest) software.
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