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Gclc hs gclc 1 sg

Manufactured by Qiagen
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The GCLc (Hs_GCLC_1_SG) is a laboratory equipment product from Qiagen. It is a tool used for the analysis of gene expression. The core function of this product is to facilitate the quantification of mRNA levels for the GCLC gene, which is involved in the synthesis of the glutamate-cysteine ligase enzyme.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Qiashredder, by Qiagen (2 mentions) Gotaq qpcr master mix, by Promega (2 mentions) Gapdh, by Qiagen (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Lightcycler 480 system, by Roche (2 mentions) Egr1 hs egr1 1 sg, by Qiagen (2 mentions) Ho 1 hs hmox1 1 sg, by Qiagen (2 mentions)

2 protocols using gclc hs gclc 1 sg

1

Quantification of Antioxidant Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
QIAshredder and RNeasy Mini Kit (Qiagen, Hilden, Germany) were used to isolate RNA according to the manufacturer’s protocol. After determining RNA concentration, one μg RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the supplier’s manual. For gene quantification, six ng cDNA per template was used with GoTaq qPCR Master Mix (Promega, Vienna, Austria) and gene specific primers. The qPCR protocol was performed by LightCycler 480 system (Roche Diagnostics, Vienna, Austria). The following gene specific primers were used: GAPDH (Hs_GAPDH_1_SG), Nrf2 (Hs_NFE2L2_1_SG), Egr1 (Hs_EGR1_1_SG), HO-1 (Hs_HMOX1_1_SG) and GCLc (Hs_GCLC_1_SG) (Qiagen). Relative gene expression levels were normalized to GAPDH and calculated using ΔΔCT method.
Koyani C.N., Kitz K., Rossmann C., Bernhart E., Huber E., Trummer C., Windischhofer W., Sattler W, & Malle E. (2016). Activation of the MAPK/Akt/Nrf2-Egr1/HO-1-GCLc axis protects MG-63 osteosarcoma cells against 15d-PGJ2-mediated cell death. Biochemical pharmacology, 104, 29-41.
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2

Quantification of Antioxidant Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
QIAshredder and RNeasy Mini Kit (Qiagen, Hilden, Germany) were used to isolate RNA according to the manufacturer’s protocol. After determining RNA concentration, one μg RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the supplier’s manual. For gene quantification, six ng cDNA per template was used with GoTaq qPCR Master Mix (Promega, Vienna, Austria) and gene specific primers. The qPCR protocol was performed by LightCycler 480 system (Roche Diagnostics, Vienna, Austria). The following gene specific primers were used: GAPDH (Hs_GAPDH_1_SG), Nrf2 (Hs_NFE2L2_1_SG), Egr1 (Hs_EGR1_1_SG), HO-1 (Hs_HMOX1_1_SG) and GCLc (Hs_GCLC_1_SG) (Qiagen). Relative gene expression levels were normalized to GAPDH and calculated using ΔΔCT method.
Koyani C.N., Kitz K., Rossmann C., Bernhart E., Huber E., Trummer C., Windischhofer W., Sattler W, & Malle E. (2016). Activation of the MAPK/Akt/Nrf2-Egr1/HO-1-GCLc axis protects MG-63 osteosarcoma cells against 15d-PGJ2-mediated cell death. Biochemical pharmacology, 104, 29-41.
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