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Eutech ph 700 meter

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Eutech pH 700 Meter is a digital pH meter designed for accurate and reliable pH measurement. It features a large, easy-to-read LCD display and provides pH, temperature, and mV measurements. The meter is equipped with automatic temperature compensation and can store up to 50 data points for later review.

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5 protocols using eutech ph 700 meter

1

Physicochemical Characterization of Frozen Passion Fruit Juice

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All the above mentioned frozen PPJ samples were analyzed for pH, free acidity, soluble solids, dry matter and protein content.
The pH was measured using a Eutech pH 700 Meter (Thermo Fisher Scientific Inc., Waltham, MA, USA) and by placing a pH probe (Eutech pH 700 Meter) directly in the sample to be analyzed.
The free acidity, expressed as citric acid (%) was determined on 10 mL of the sample homogenized with 20 mL of distilled water by titrating with NaOH (0.1 N) up to pH 8.1.
The soluble solid content of each sample was determined using a refractometer and expressed as °Brix; moreover, the content of reducing sugar was analyzed by using the Fehling’s solution method [13 ].
The dry matter percentage (DM%) was determined by drying the sample at 105 °C until constant weight with an electronic moisture balance (Eurotherm, Gibertini®, Novate Milanese, Italy).
Protein and fat were determined using standard AOAC methods [14 ].
All the analyses were carried out in triplicate and results were expressed as mean ± standard deviation.
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2

Fruit Slice pH, Acidity, and Vitamin C

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The pH of fruit slices was determined using approved methods of the Association of Official Analytical Chemists. The Eutech pH-700 meter (ECFC7252101B-electrode, Thermo Fisher Scientific, China) was used in pH determination. Acidity was determined using standard laboratory methods of analysis which involve measuring 10 ml of sample into a 250 ml conical flask, adding 100 ml distilled water and 3 drops of 1% phenolphthalein indicator, and finally titrating against 0.1% N sodium hydroxide until the solution turned pink. Color values were attained by placing small pieces of mango slices unto the illuminated slide of the Lovibond Tintometer Colorimeter (Model F, UK) while adhering to standard procedures. Vitamin C values were obtained through standard titration procedures [35 ]. Figure 2 displays some of the equipment that were used in data collection and analyses.
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3

Measuring pH Changes During Fermentation

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The pH of beverages was determined throughout the fermentation using a digital pH meter (Eutech pH 700 meter, Thermo Fisher Scientific Inc., Waltham, MA, United States) with a glass electrode (Electrode ECFC7252101B, Thermo Fisher Scientific Inc, Waltham, MA, United States). Before measurement, the pH meter was calibrated with buffers (Thermo Fisher Scientific Inc, New Zealand) at pH 4.0 and 7.0. pH determination was performed in triplicates for each fermented beverage sample (20 mL) at 0, 12, 24, 48, and 72 h.
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4

Measuring pH of MD2-PPC Extracts

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The pH value of MD2-PPC was measured using a pH meter (Thermo Scientific™ Eutech™ pH 700 Meter, Fischer Scientific, Lougborough, Leicestershire, UK). The probe was standardized before use with pH 4 and pH 7 buffers. Peel and core extracts (10 mL) were placed in a 50 mL beaker and measured using a glass electrode probe. Analysis was performed in triplicate.
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5

Comprehensive Water Quality Assessment

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The air temperature, water temperature and conductivity were measured on-site using a portable EC/TDS TEMP Waterproof Combo Meter (C-100, HM Digital Inc., Culver City, CA, USA). The pH was measured using a portable Eutech pH700 meter (Thermo Fisher Scientific Inc., Waltham, MA, USA). One hundred ml of water sample in triplicate was filtered using glass fiber filters (Millipore, St. Louis, MO, USA); Chlorophyll a (Chl a) was extracted using 90% acetone and measured by spectrophotometry following the method of American Public Health Association (APHA, 2005 ). The samples for nutrient analysis were stored at −20°C soon after arrival to the lab and the parameters such as total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), nitrite nitrogen (NO2-N), ammonium nitrogen (NH4-N), phosphate (PO4-P), and total organic carbon (TOC) were measured as reported in our earlier studies (Vadde et al., 2018 (link); Yuan et al., 2019 (link)). All the values of physico-chemical parameters were compared to the class III water quality standards set by the Chinese Ministry of Environmental Protection (now called as Ministry of Ecology and Environment, People’s Republic of China) (MEP, 2002 ) and correlated with the results of ARGs.
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