Betulinic acid was purchased from YuanYe biotechnology (Shanghai, USA) and dissolved in DMSO as 100 mM. Counting Kit-8 (CCK-8) assay and Annexin V-FITC Apoptosis kit were obtained from BestBio Company (Shanghai, China). mTOR antibody (ab2732), Caspase-3 antibody (ab2302), p62 antibody (ab155686) were provided by Abcam. After that, S6K1 antibody (CST 9202), p-S6K1 antibody (CST 9204S), AMPK antibody (CST 2532S), p-AMPKα1 antibody (CST 2537), p-mTOR antibody (CST 5536S), Caspase8 antibody (CST 4790), Bax antibody (CST 5023S) and LC3A/B antibody (CST 12741) were bought from Cell Signaling Technology. Bcl2 antibody (12789–1-AP) and GAPDH antibody (AP0063) were acquired from Proteintech and Bioworld Technology, respectively.
S6k1 antibody
Manufactured by Cell Signaling Technology
The S6K1 antibody is a laboratory reagent used in research applications. It is a specific antibody that recognizes the S6K1 (ribosomal protein S6 kinase beta-1) protein. S6K1 is a protein kinase that plays a role in the regulation of cell growth and proliferation. The S6K1 antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the S6K1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Bax antibody, by Cell Signaling Technology (1 mentions)
Caspase 8 antibody, by Cell Signaling Technology (1 mentions)
Ampk antibody, by Cell Signaling Technology (1 mentions)
P mtor antibody, by Cell Signaling Technology (1 mentions)
Bcl2 antibody 12789 1 ap, by Proteintech (1 mentions)
Betulinic acid, by Yuanye Bio-Technology (1 mentions)
Lc3a b antibody, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc apoptosis kit, by BestBio (1 mentions)
Hif 1α antibody, by Cell Signaling Technology (1 mentions)
β actin antibody, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 antibody 9661, by Cell Signaling Technology (1 mentions)
Sp600125, by Selleck Chemicals (1 mentions)
2 protocols using s6k1 antibody
1
Betulinic Acid: Apoptosis and mTOR Pathway Modulation
2
Caspase and JNK Inhibitor Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The caspase-3 inhibitor Z-VAD-fmk and the caspase-9 inhibitor Z-LEHD-fmk were purchased from Calbiochem (Shanghai, China). The two JNK (Jun N-terminal protein kinase) inhibitors, SP600125 and JNK inhibitor IX (JNKi-IX), were purchased from Selleck (Shanghai, China). p-AKT (Ser 473) antibody (9271), AKT1 antibody (2938), p-S6 ribosomal protein (S6, Ser 235/236) antibody (2211), S6 antibody (2317), p-p70 S6 kinase 1 (S6K1, Thr 398) antibody (9209), S6K1 antibody (9202), p-SAPK/JNK1 (Thr183/Tyr185) antibody (9251), Jun N-terminal protein kinase (JNK1) antibody (3708), p-ASK1 (Thr 845) antibody (3765), ASK1 antibody (3762), cyclin D1 antibody (2922), HIF-1α antibody (3716), c-Jun antibody (9165) and (β-)Tubulin (D2N5G) antibody (15115), cleaved-caspase-3 antibody (9661) and β-actin antibody (3700) were all purchased from Cell Signaling Tech.(Beverly, MA).
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