Oridonin (≥98%, HPLC) was purchased from mingwang biotechology (China). FBS, penicillin/streptomycin, DMEM medium, and trypsin kit were obtained from Gibco (USA). EGF was purchased from R&D (USA) and anti-EGFR antibody was obtained from Cell Signaling (USA). N-acetyl-l-cysteine (NAC), Annexin V-FITC/PI (Annexin V-Fluorescein Isothiocyanate/Propidium Iodide) apoptosis detection kit and DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) ROS assay kit were purchased from Beyotime Institute of Biotechnology. RIPA lysis buffer, Anti-EGFR IgG, anti-β-actin IgG, anti-rabbit IgG were from Cell Signaling (USA).
Anti egfr igg
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-EGFR IgG is a laboratory reagent that detects the Epidermal Growth Factor Receptor (EGFR) protein. It is an antibody that specifically binds to EGFR, allowing for its identification and quantification in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin kit, by Thermo Fisher Scientific (1 mentions)
Anti egfr antibody, by Cell Signaling Technology (1 mentions)
Anti β actin igg, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by R&D Systems (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Ros assay kit, by Beyotime (1 mentions)
Apoptosis detection kit, by Beyotime (1 mentions)
Lysis buffer, by Cell Signaling Technology (1 mentions)
Anti phospho p44 42 mapk perk1 2, by Cell Signaling Technology (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
2 protocols using anti egfr igg
1
Oridonin Cytotoxicity and EGFR Modulation
2
Western Blot Characterization of Stem Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells were harvested and lysed with the lysis buffer (Cell Signalling). Samples were resolved on SDS-PAGE gels and transferred onto PVDF membranes, which were blocked with 5% BSA, and incubated with primary and HRP-conjugated secondary IgGs, followed by visualisation using the ChemiDoc XRS+ System (Biorad). Primary antibodies were as follows: anti-CD24 IgG, anti-EpCAM IgG (Abcam), anti-CD47 IgG (Santa Cruz), anti-Oct3/4 IgG (Stem Cell Technologies), anti-ABCG2 IgG, anti-phospho-p44/42 MAPK (pERK1/2) (Thr202/Thr204) IgG, anti-p44/p42 (ERK1/2) IgG, anti-P38 MAPK IgG, anti-phospho-P38 MAPK (pP38) (Thr180/Thr182) IgG, anti-EGFR IgG, anti-phospho-EGFR (pEGFR) (Tyr1068) IgG (all Cell Signaling). Anti-actin IgG (Sigma) was used as a loading control.
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