Micromhm525 nx cryostat

Manufactured by Thermo Fisher Scientific

The MicromHM525 NX Cryostat is a laboratory instrument used for the preparation of thin tissue sections for microscopic analysis. It maintains a controlled low-temperature environment to enable the cutting and mounting of samples.

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Tissue tek oct compound, by Ted Pella (1 mentions)

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Cryostat (177 citations)

2 protocols using micromhm525 nx cryostat

Laser-Capture Microdissection and qPCR Analysis of GBM Tumor Cells

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Snap-frozen and OTC-embedded (Tissue-Tek® OCT Compound, Ted
Pella, INC., Redding, CA) of 15 GBM specimens were sectioned using a Microm
HM525 NX Cryostat (Thermo Fisher Scientific Inc., Grand Island, NY); one of
many serial consecutive sections per case was stained with hematoxylin and
eosin to confirm the localizations of tumor cells in different regions.
Tumor cells in different regions were subsequently isolated by laser-capture
microdissection using a Zeiss PALM (Carl Zeiss, Inc., Thornwood, NY). For
each case, 3 regions of tumor cells were separately collected for RNA
extraction. Total RNA was isolated from the cells using TRIzol RNA Isolation
Reagent according to the manufacturer’s instructions. The
concentrations and quality of the recovered RNA were measured using a
NanoDrop 2000 spectrophotometer (Nanodrop Technologies, Wilmington, DE). For
quantitative analysis of mRNA expressions of FOXM1-AS and FOXM1 by qPCR,
mRNA levels were normalized to GAPDH mRNA.

Zhang S., Zhao B.S., Zhou A., Lin K., Zheng S., Lu Z., Chen Y., Sulman E.P., Xie K., Bögler O., Majumder S., He C, & Huang S. (2017). The m6A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-Like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program. Cancer cell, 31(4), 591-606.e6.

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Mice DRG Tissue Preparation

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For all fluorescence imaging and immunohistochemistry (IHC) experiments, mice were deeply anesthetized with isoflurane before being transcardially perfused with 10 mL of phosphate-buffered saline (PBS), followed by 25 mL of 4 % PFA in 0.1 M phosphate buffer. Their L3 and L4 DRGs were dissected and post-fixed in 4 % PFA for 1 h, followed by cryoprotection in a 30 % sucrose solution in PBS for 72 h. The DRGs were then embedded in optimal cutting temperature compound (OCT) and cut into 14 µm-thick transverse sections with a cryostat (Thermo Scientific, Waltham, MA, Microm HM525 NX Cryostat) directly onto microscope slides.

Mouchbahani-Constance S., Lagard C., Schweizer J., Labonté I., Georgiopoulos M., Otis C., St-Louis M., Troncy E., Sarret P., Ribeiro-Da-Silva A., Ouellet J.A., Séguéla P., Paquet M.E, & Sharif-Naeini R. (2023). Modulating the activity of human nociceptors with a SCN10A promoter-specific viral vector tool. Neurobiology of Pain, 13, 100120.

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