Meso 2 3 dimercaptosuccinic acid

CoQ₁₀powder (98%), sodium meta-arsenite (Trivalent-inorganic arsenic) and Meso-2,3-Dimercaptosuccinic Acid (98%) were purchased from Sigma Aldrich (St Louis, MO). CoQ₁₀was prepared by dissolving it in olive oil solution. On the other hand sodium meta-arsenite and Meso-2,3-Dimercaptosuccinic Acid were prepared by dissolving them in deionized water.

Meso-2,3-dimercaptosuccinic acid, ~98%, (DMSA) was obtained from Sigma Aldrich (St. Louis, MO).
Green tea extract (Nestle) was a gift of Mario Ferruzzi, Department of Food Science, Purdue University. The green tea polyphenol concentration in gallic acid equivalents (GAE) was 435 mg GAE/g green tea as analyzed by the Folin assay [16 ]. The catechin content of the green tea was determined by HPLC-ECD as described by Peters et al. [17 (link)]. Each gram of green tea contained 125 mg epigallocatechin, 30 mg epicatechin, 243 mg epigallocatechin gallate, and 35 mg epicatechin gallate.
Green tea and DMSA were analyzed for mercury contamination using the DMA-80 Mercury Analyzer (Milestone, Inc., Monroe, CT). Green tea contained 0.458 ± 0.032 ng/g and DMSA contained 5.361 ± 0.087 ng/g.

In the present work, chelators in a completely basic form (all acidic functional groups were titrated with Na⁺) were impregnated into the macroporous silica support using their aqueous solutions (typically, 0.25–2.0 wt% chelator concentrations). For the preparation of the aqueous solutions, trisodiumphosphate (1 in Fig. 3a, Sigma Aldrich, 96%) and ethylenediaminetetraacetic acid tetrasodium salt dihydrate (4, Sigma Aldrich, 99%) were directly dissolved in deionized water. In the cases of chelators in an acidic form, such as 1-hydroxyethane 1,1-diphosphonic acid monohydrate (2, Sigma Aldrich, 95%), ethylenediamine tetramethylene phosphonic acid (3, Tokyo Chemical Industry, 98%), diethylenetriaminepentaacetic acid (5, Sigma Aldrich, 99%), and meso-2,3-dimercaptosuccinic acid (6, Sigma Aldrich, 98%), controlled amounts of NaOH were dissolved together in deionized water to completely titrate acidic H⁺ into Na⁺. After the wet impregnation of the chelators into the silica support, the samples were dried in a vacuum oven at 100 °C for 12 h. The final loading of chelators was controlled in the range of 0–4 wt% with respect to the mass of silica support.