2 4 6 trinitrobenzenesulfonic acid solution

Laboratory chemicals, namely 5-aminosalicylic acid, 2,4,6-trinitrobenzenesulfonic acid solution (TNBS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), glutathione reductase, L-glutathione reduced (GSH) and oxidized (GSSG) forms, β-NADPH, 5-sulfosalicylic acid, 2-vinylpyridine, 2,3-diaminonaphthalene (DAN), o-dianisidine dihydrochloride, hexadecyltrimethylammonium bromide (CTAB), protease inhibitor cocktail and general laboratory chemicals were purchased from Sigma-Aldrich (St Louis, Missouri, USA). Microcon-10kDa centrifugal filters were obtained from Merck Millipore (Merck KGaA, Darmstadt, Germany).
Rabbit polyclonal antibody to iNOS was purchased from Cell Signalling Technology (Leiden, The Netherlands); rabbit polyclonal antibody to COX-2 was purchased from Abcam (Cambridge, UK); goat polyclonal antibody to MPO was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal antibody to β-actin was purchased from Sigma-Aldrich (St Louis, Missouri, USA); anti-rabbit, anti-mouse and anti-goat IgG secondary antibodies were purchased from Abcam (Cambridge, UK).

Papain (specific activity of ≥30,000 U/mg), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and 2,4,6-trinitrobenzenesulfonic acid solution (TNBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrogen peroxide was purchased from Merck (Darmstadt, Germany). Disodium hydrogen phosphate dihydrate was purchased from RCI Labscan (Bangkok, Thailand). Sodium dihydrogen phosphate dihydrate, 1,10-phenanthroline, and ferrous sulfate heptahydrate were purchased from QReC (Quality Reagent Chemical, Auckland, New Zealand). Peroxidase (horseradish, specific activity of 24 U/mg) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents used in the experiment were of analytical grade.