Myc tag

Manufactured by ABclonal

Sourced in United Kingdom

Myc-tag is a short, synthetic peptide sequence that can be fused to a protein of interest to facilitate its detection and purification. The Myc-tag is widely used in molecular biology and biochemistry research as a reliable and versatile tool for protein analysis.

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Lab products found in correlation

Ha tag, by ABclonal (1 mentions) Actin, by Proteintech (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Flag tag (flag), by Proteintech (1 mentions) Ab6302, by Abcam (1 mentions) Quantity one 4, by Bio-Rad (1 mentions) Tris buffered saline (tbs), by Solarbio (1 mentions) Skim milk, by Solarbio (1 mentions) Af5003, by Beyotime (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions) A0208, by Beyotime (1 mentions) Ab222320, by Abcam (1 mentions) Cell lysis buffer for western and ip, by Beyotime (1 mentions) Ha tag, by Abcam (1 mentions) Hp mlkl, by Abcam (1 mentions) Gfp tag, by ABclonal (1 mentions) Sting, by Proteintech (1 mentions) Flag tag (flag), by ABclonal (1 mentions) Gapdh, by Proteintech (1 mentions)

4 protocols using myc tag

Antibody Inventory for Protein Analysis

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Primary antibodies for USP29 (Cat# AP2153c, Abcepta), AURKB (Cat# 3094, CST), FUBP1 (Cat# A5587, Abclonal), Flag-tag (Cat# 20543-1-AP, Proteintech), HA-tag (Cat# AE008, Abclonal), Myc-tag (Cat# AE009, Abclonal), ACTIN (Cat# 66009-1-Ig, Proteintech) were purchased from indicated companies. Cycloheximide (CHX; Cat# 01810) was obtained from Sigma Aldrich.

Tu R., Kang Y., Pan Y., Da Y., Ren D., Zhang R., Cai Z., Liu Y., Xu J., Ma J., Zhou Z., Yin S., Li X., Zhang P., Zhang Q., Wang J., Lu X, & Zhang C. (2024). USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer. Cancer Cell International, 24, 33.

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Western Blot Analysis of Protein Interactions

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Whole-cell lysate and the Co-IP supernatant were mixed with 5× SDS-PAGE Plus sample buffer (GenStar, Beijing, China) and then incubated in a boiling water bath before separation by SDS-PAGE and subsequent transfer to PVDF membrane (Millipore, Bedford, MA). The membrane was blocked with 5% skim milk (Solarbio, Beijing, China) for 3 h then incubated with primary antibody overnight at 4°C. The membrane was washed thrice for 10 min each time using Tris-buffered saline and 0.1% Tween-20 (Solarbio, Beijing, China) and incubated with the appropriate secondary antibody. The primary antibodies used for Western blot were β-catenin rabbit polyclonal antibody (1:2,000) (ab6302, Abcam, Cambridge, UK), Myc-tag rabbit monoclonal antibody (1:5,000) (AE070, ABclonal), and HA-tag rabbit monoclonal antibody (1:1,000) (C29F4, Cell Signaling). β-actin rabbit monoclonal antibody (1:2,000) (AF5003, Beyotime) was used as a loading control. The secondary antibody was goat anti-rabbit IgG (1:5,000) (A0208, Beyotime). The blot signal was visualized using ECL reagent (Thermo Fisher Scientific, Carlsbad, CA). The protein expression level was quantified by the band density using Quantity One 4.6.3 software (Bio-Rad Laboratories, Hercules, CA) and normalized by β-actin.

Ma X., Han X., Wang W., Zhang Q, & Tang H. (2023). β-Catenin regulates ovarian granulosa cell cycle and proliferation in laying hens by interacting with TCF4. Poultry Science, 103(3), 103377.

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Immunoprecipitation and Western Blot Analysis

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Cells were treated with cell lysis buffer for Western and IP (Beyotime). The indicated antibodies were added to cell lysates or supernatant, followed by incubation with gentle rocking overnight at 4°C. Then, protein G agarose beads were added to cell lysates, which was incubated with gentle rocking for 4 h at 4°C. Next, samples were eluted in SDS‐PAGE sample loading buffer and boiled. For immunoprecipitation, anti‐FLAG (AE005; ABclonal), STING (19851‐1‐AP; Proteintech) and MLKL (PA5‐102810; Invitrogen) antibodies were used.
The primary antibodies were used including STING (19851‐1‐AP; Proteintech), hp‐STING (S366, 50907; CST), mp‐STING (S365, 72971; CST), p‐TBK1 (S172; CST), TBK1 (38066; CST), IRF3 (4302; Cell Signaling), p‐IRF3 (S396, 29047; CST), mp‐RIPK3 (T231+S232, ab222320; Abcam), mp‐MLKL(S345, ab196436; Abcam), hp‐RIPK3 (S227, ab209384; Abcam), hp‐MLKL (S358, ab187091; Abcam), hMLKL (A19685; ABclonal), mMLKL (37705; CST), hRIPK3 (86671; CST), mRIPK3 (15828; CST), mp‐RIPK1 (S166, 31122; CST), RIPK1 (3493; CST), LC3 (12741; CST), P62 (A7758; ABclonal), β‐actin (4970; CST), β‐Tubulin (AC021; ABclonal), GAPDH (60004‐1‐Ig; Proteintech), FLAG‐tag (AE063; ABclonal), HA‐tag (ab236632; Abcam), MYC‐tag (AE070; ABclonal) and GFP‐tag (AE012; ABclonal).

Zhang X., Wu J., Liu Q., Li X., Yang Y., Wu L., Wu X., Zhao Y, & Ren J. (2023). RIPK3–MLKL necroptotic signalling amplifies STING pathway and exacerbates lethal sepsis. Clinical and Translational Medicine, 13(7), e1334.

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Western Blot Antibody Characterization

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The following antibodies were used for western blotting: KLHL7 (Atlas Antibodies HPA029491, 1:1000); RASA2 (Abclonal, A18375, 1:1000); P-ERK1/2 (Cell Signaling Technology, 4376, 1:1000); P-MEK1/2 (Cell Signaling Technology, 9154, 1:500); ERK1/2 (Cell Signaling Technology, 9102, 1:500); MEK1/2 (Cell Signaling Technology, 9122, 1:1000); pan-RAS (Abclonal, A19779, 1:1000); FLAG (Milliporesigma, F3165, 1:4000; Cell Signaling Technology, 14793, 1:4000); myc-tag (Abclonal, AE010, 1:4000); HA-tag(Abmart, M20003, 1:4000) and GST-tag (Abmart, M20007, 1:4000). Both MG-132 and CHX were purchased from Selleck.

Chen L., Li Y, & Chen Y. (2023). KLHL7 promotes hepatocellular carcinoma progression and molecular therapy resistance by degrading RASA2. iScience, 26(6), 106914.

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