Kn 93

Maresin‐1 and Maresin‐1 ELISA kit were purchased from Cayman Chemical. AngII was purchased from Enzo Life Sciences. Mouse IL‐1β ELISA kit was purchased from Neobioscience. EdU imaging kits was purchased from APExBIO. ML385 and KN‐93 were purchase from Topscience. Fluo‐4 AM was purchased from Invitrogen. Fetal bovine serum was purchased from Inner Mongolia Opcel Biotechnology Co., Ltd. Primary antibodies included: anti‐GAPDH (Abcolonal), anti‐SM22α (Servicebio), anti‐ASC (Santa Cruz Biotechnology), anti‐caspase‐1 p20 (Santa Cruz Biotechnology), anti‐IL‐1β (Santa Cruz Biotechnology), anti‐OPN (Immunoway), anti‐NLRP3 (Immunoway), anti‐IL‐18 (ImmunoWay), anti‐GSDMD‐N (Immunoway), anti‐Nrf2 (GeneTex), anti‐α‐SMA(Abcam), anti‐HO‐1(Abcam), anti‐CaMKII (Abcam), anti‐p‐CaMKII (Zenbio), anti‐PCNA (Cell Signaling Technology), anti‐rabbit IgG (H+L) (Cell Signaling Technology), anti‐mouse IgG (H+L) (Cell Signaling Technology), Cy3‐conjugated goat anti‐rabbit IgG (H+L) (Servicebio), and Alexa Fluor® 488‐conjugated goat anti‐rabbit IgG (H+L) (Servicebio).

The wild type (WT) A. alternata strain JT-03 was isolated from infected pear fruit and cultured on potato dextrose agar (PDA) at 28 °C for 5 d. The pCAMBIA1300-HPH and pC-NEO-NGFP vectors were provided by the Chinese Academy of Sciences. The pear wax used in the experiments was extracted from pears as described by Yin et al. [42 (link)] and then prepared as a 0.1% wax solution. KN93 and Gelbond PAG film were purchased from TopScience (Shanghai, China) and Univ-bio (Shanghai, China). The pear (Pyrus bretchneideri ‘Zaosu’) was harvested from a Tiaoshan Farm in Jingtai County, Gansu Province, China.