Ab113344

The following bone markers and proinflammatory cytokines in mouse serum were measured using commercially available kits according to the manufacturer’s manual instructions: alkaline phosphatase (ALP) (Catalog #K412–500, BioVision, Milpitas, CA, USA), osteocalcin (OC) (Catalog #SEA471Mu, USCN Life Science Inc., Wuhan, China), cross-linked C-telopeptide of type I collagen (CTX1) (Catalog #CEA665Mu, USCN Life Science Inc.), osteoprotegerin (OPG) (Catalog #MOP00, R&D Systems Inc., Minneapolis, MN, USA), receptor activator of nuclear factor κ-B ligand (RANKL) (Catalog #MTR00, R&D Systems Inc.), interleukin-1α (IL-1α) (Catalog #ab113344, Abcam, Cambridge, MA, USA), IL-1β (Catalog #ab108866, Abcam), IL-6 (Catalog #ab100712, Abcam) and tumor necrosis factor-α (TNF-α) (Catalog #ab208348, Abcam). The measurements were conducted by an automated microplate reader (Tu et al., 2021 (link)).

Fresh blood was collected and deposited at room temperature and centrifuged at 9000 g for collection of supernatants. The supernatants were stored at −20°C for later use. Murine serum norepinephrine, corticosterone, and VEGF were measured by NE (MyBioSource, MBS776673, and Abnova, KA1891), CORT (MyBioSource, MBS775946, and Abcam, ab108821), and VEGF (MyBioSource, MBS412303) ELISA Kits, respectively. Murine serum IL‐1a, IL‐6, and bFGF were measured by IL‐1a (Abcam, ab113344), IL‐6 (Abcam, ab100712), and bFGF ELISA Kits (Abcam, ab100670), respectively.