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Dnase 1 d5319

Manufactured by Merck Group
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DNase I (D5319) is a laboratory product manufactured by Merck Group. It is an enzyme that catalyzes the hydrolytic cleavage of DNA. This product is designed for use in molecular biology and biochemistry applications.

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Lab products found in correlation

Dnase 1, by Thermo Fisher Scientific (2 mentions) Collagenase 4, by Merck Group (2 mentions) Collagenase 1, by Merck Group (2 mentions) Hyaluronidase h3506, by Merck Group (2 mentions)

Spelling variants of this product

DNase I (4163 citations) DNase (1597 citations) DNase I (DNase; (2 citations)

2 protocols using dnase 1 d5319

1

Bone Marrow and Aorta Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Before organ collection, mice were perfused with 20 ml ice-cold PBS. The epiphysis of the femurs and tibiae were opened, flushed with FACS buffer and filtered through a 40 μm cell strainer. Marrow was collected by flushing the plug out of the bones. The supernatant was removed and digested with collagenase IV (Sigma-Aldrich, C5138); dispase (Gibco by Life Technology, 17105–041) and DNase I (Thermo Scientific, 90083). Aortas were excised, minced and enzymatically digested with collagenase I (C0130), collagenase XI (C7657), DNase I (D5319) and hyaluronidase (H3506, all Sigma-Aldrich).
Schloss M.J., Hulsmans M., Rohde D., Lee I.H., Severe N., Foy B.H., Pulous F.E., Zhang S., Kokkaliaris K.D., Frodermann V., Courties G., Yang C., Iwamoto Y., Knudsen A.S., McAlpine C.S., Yamazoe M., Schmidt S.P., Wojtkiewicz G.R., Masson G.S., Gustafsson K., Capen D., Brown D., Higgins J.M., Scadden D.T., Libby P., Swirski F.K., Naxerova K, & Nahrendorf M. (2022). B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis. Nature immunology, 23(4), 605-618.
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2

Bone Marrow and Aorta Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Before organ collection, mice were perfused with 20 ml ice-cold PBS. The epiphysis of the femurs and tibiae were opened, flushed with FACS buffer and filtered through a 40 μm cell strainer. Marrow was collected by flushing the plug out of the bones. The supernatant was removed and digested with collagenase IV (Sigma-Aldrich, C5138); dispase (Gibco by Life Technology, 17105–041) and DNase I (Thermo Scientific, 90083). Aortas were excised, minced and enzymatically digested with collagenase I (C0130), collagenase XI (C7657), DNase I (D5319) and hyaluronidase (H3506, all Sigma-Aldrich).
Schloss M.J., Hulsmans M., Rohde D., Lee I.H., Severe N., Foy B.H., Pulous F.E., Zhang S., Kokkaliaris K.D., Frodermann V., Courties G., Yang C., Iwamoto Y., Knudsen A.S., McAlpine C.S., Yamazoe M., Schmidt S.P., Wojtkiewicz G.R., Masson G.S., Gustafsson K., Capen D., Brown D., Higgins J.M., Scadden D.T., Libby P., Swirski F.K., Naxerova K, & Nahrendorf M. (2022). B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis. Nature immunology, 23(4), 605-618.
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