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Quant it dsdna broad range assay kit

Manufactured by Agilent Technologies
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The Quant-IT™ dsDNA Broad-Range assay kit is a fluorescence-based solution for quantifying double-stranded DNA (dsDNA) in a broad concentration range. The kit provides a sensitive and accurate method for determining the concentration of dsDNA samples.

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Lab products found in correlation

Qubit 3 fluorometer, by Thermo Fisher Scientific (2 mentions) Synergy htx, by Agilent Technologies (2 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (2 mentions) Janus liquid handler workstation, by PerkinElmer (1 mentions)

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Broad range kit (2 citations)

2 protocols using quant it dsdna broad range assay kit

1

DNA Extraction and Quantification

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All DNA samples were extracted from the buffy coats isolated from blood samples of the participants and were provided by the CEPH and CNRGH Biobanks. DNA samples from all collections were quantified using Quant-IT™ dsDNA Broad-Range assay kit on a Synergy HTX (BioTek) or Qubit™ dsDNA BR assay Kit on a Qubit 3 Fluorometer (Thermo Fischer Scientific), according to the manufacturer’s instructions.
Daunay A., Hardy L.M., Bouyacoub Y., Sahbatou M., Touvier M., Blanché H., Deleuze J.F, & How-Kit A. (2022). Centenarians consistently present a younger epigenetic age than their chronological age with four epigenetic clocks based on a small number of CpG sites. Aging (Albany NY), 14(19), 7718-7733.
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2

DNA Quantification and Normalization

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DNA from all collections was quantified using Quant-IT™ dsDNA Broad-Range assay kit on a Synergy HTX (BioTek) for fluorescence measurement and analysis (Centre de Ressources Biologiques, CEPH Biobank, Foundation Jean Dausset—CEPH) or Qubit™ dsDNA BR assay Kit on a Qubit 3 Fluorometer (Thermo Fischer Scientific), according to the manufacturer’s instructions. DNA sample concentrations were equalized to 5 ng/µL and dispensed in 96 wells PCR plates using a JANUS Liquid Handler Workstation (Perkin Elmer).
Hardy L.M., Bouyacoub Y., Daunay A., Sahbatou M., Baudrin L.G., Gressin L., Touvier M., Blanché H., Deleuze J.F, & How-Kit A. (2022). A high-throughput real-time PCR tissue-of-origin test to distinguish blood from lymphoblastoid cell line DNA for (epi)genomic studies. Scientific Reports, 12, 4684.
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