Anerocult c

Aliquots of 10 mL were collected from each pomegranate juice (after homogenisation by shaking thoroughly) at various time intervals during fermentation and storage. The samples were blended with 90 mL of sterile 1/4 strength Ringer’s solution (Sigma-Aldrich) and mixed in a stomacher blender and subjected to serial decimal dilutions in 1/4 strength Ringer’s solution. Viable counts of lactobacilli, yeasts and fungi, and coliforms were determined in triplicate by plating appropriate dilutions on the selective media for each species [31 (link)]. Specifically, viable counts of Lactobacillus plantarum were enumerated on acidified MRS agar (Merck, Darmstadt, Germany) at 37 °C for 72 h, anaerobically (Anaerobic jar, Anerocult C, Merck, Darmstadt, Germany). Coliforms were enumerated on Violet Red Bile agar (Lab M, Lancashire, UK) after incubation at 30 °C for 24 h. Yeasts and fungi were determined by plating on Sabouraud Chloramphenicol Agar (Merck, Germany) after incubation at 30 °C for 72 h. All cell counts were expressed as log of mean colony forming units (cfu) per mL of pomegranate juice. All results are presented as means of three repetitions plus standard deviations.

Samples of 10 mL were taken from the Cornelian cherry juice at various time intervals (days 1, 7, 14, 21 and 28) during fermentation and cold storage. The samples were blended with 90 mL of sterile ¼ strength Ringer’s solution (Merck, Taufkirchen, Germany) and mixed in a stomacher blender and then subjected to serial decimal dilutions in a ¼ strength Ringer’s solution. L. plantarum ATCC 14917 cells were recorded on acidified MRS agar (Merck, Taufkirchen, Germany) at 37 °C for 72 h, anaerobically (Anaerobic jar, Anerocult C, Merck, Taufkirchen, Germany). All cell counts were expressed as the log of mean colony forming units (CFU) per mL of Cornelian cherry juice. The results are presented as means of three repetitions plus standard deviations.

Aliquots of 10 mL were retrieved from each fermented flask of chokeberry juice after thorough homogenization at various time intervals during the 4 weeks at 4 °C. Each sample was serially diluted in 90mL of sterile Ringer’s solution of one-quarter strength (Sigma-Aldrich, Darmstadt, Germany), placed aseptically in a sterile plastic bag and homogenized in a Bagmixer (400 Model VW, Interscience). Subsequently the suspension was then subjected to serial decimal dilutions of one-quarter strength Ringer’s solution and plated on selective media.
Viable cell counts of the potentially probiotic strain (L. paracasei SP5) were enumerated on acidified MRS agar (Merck, Darmstadt, Germany) at 37 °C for 72h under anaerobic conditions (anaerobic jar, Anerocult C, Merck, Germany). Yeasts and fungi were enumerated on potato dextrose agar (PDA) after incubation at 30 °C for 72 h. Possible coliform cell counts were examined on Violet Red Bile Agar (VRBA) after incubation at 30 °C for 24 h [27 (link)].
All media were prepared according to the instructions provided by the manufacturer, were sterilized (120 °C, for 15 min, 1–1.5 atm), and then cooled to 45 °C prior to use. Cell counts were expressed as log of mean colony-forming units (cfu) per mL of chokeberry juice.