Utm media

Manufactured by Copan

Sourced in Italy

UTM media is a transport and storage medium designed for the preservation and transport of clinical samples. It maintains the viability of microorganisms during collection, storage, and shipping.

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Lab products found in correlation

Magna pure lc system, by Roche (2 mentions) Uvt media, by BD (2 mentions) Total nucleic acid isolation kit, by Roche (2 mentions) Vero ccl 81 cells, by American Type Culture Collection (2 mentions) Nuclisens easymag system, by bioMérieux (1 mentions)

Close spelling variants of this product

UTMTM Viral Transport Media Universal Transport Media (UTM, UTM viral transport media

6 protocols using utm media

Influenza Virus Detection from Nasopharyngeal Swabs

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Example 1

Samples are taken from nasopharyngeal swabs (NPS) of patients presenting flu-like symptoms and the samples are each placed into approximately 3 ml of viral transport medium (for example M4, M4RT, M5, or M6 media (Remel), UVT media (Becton Dickinson), or UTM media (Copan)). Samples were refrigerated for transport at 2-8° C., and stored at that temperature for up to approximately 72 hours before processing. Samples which needed longer storage were stored at ≤−70° C. Nucleic acids were extracted from samples using standard laboratory methods to isolate nucleic acids (e.g., MagNA Pure LC System using the Total Nucleic Acid Isolation Kit (Roche) or the NucliSENS easyMAG System using the Automated Magnetic Extraction Reagents (bioMérieux)). A positive control sample is included which has a target sequence for each of the tested viral strains. That is, if the swine H1N1 influenza A virus is to be tested, a swine H1N1 influenza A virus target sequence is included. Here, the positive controls were pooled RNA transcript from a portion of an HA gene or of an NP gene for each of the subtypes of influenza. In addition, a negative control including the viral transport medium, but not including a target sequence, and Internal Controls were extracted alongside the samples.

US10815538B2. Compositions and assays to detect seasonal H3 influenza A virus nucleic acids (2020-10-27). GEN-PROBE PRODESSE, INC. [US]. Inventors: Ejan Tyler [US].

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Isolation and Characterization of SARS-CoV-2 Strain UCN19

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SARS-CoV-2 viral strain UCN19 was isolated in March 2020, during the course of the active epidemic, from naso-pharyngeal flocked swabs obtained at the University Hospital of Caen, Normandy, France, from patients who were suffering from respiratory infection and were confirmed infected by SARS-CoV-2 by routine molecular diagnosis. The swabs were eluted in UTM media (Copan) at 4 °C for less than 48 h. Vero CCL-81 cells (passage 32, from ATCC), grown at 80 % confluence were inoculated with 200 µl micro-filtered elution for passage 0 (P0). Cells were visually checked for cytopathic effect on a daily basis using an inverted microscope. Cell supernatants (12 ml) were harvested on day 3 after inoculation and immediately used for passage 1 (P1) produced in T75 culture flasks containing Vero cells as previously described for P0. P1 was used for stock production of UCN19, aliquoted and stored at −80 °C before titration, genomic quantification, sequencing (GISAID access number EPI_ISL_666870) and experimental infections to ferrets and hamsters. The use of the low-passage SARS-CoV-2 clinical isolate P1 reduces the risk of cell-culture-induced genetic modification. All animals in this study were inoculated with inocula from the same stock.

Monchatre-Leroy E., Lesellier S., Wasniewski M., Picard-Meyer E., Richomme C., Boué F., Lacôte S., Murri S., Pulido C., Vulin J., Salguero F.J., Gouilh M.A., Servat A, & Marianneau P. (2021). Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2. The Journal of General Virology, 102(3), 001567.

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Influenza A Virus Detection Protocol

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Example 1

Samples are taken from nasopharyngeal swabs (NPS) of patients presenting flu-like symptoms and the samples are each placed into approximately 3 ml of viral transport medium (for example M4, M4RT, M5, or M6 media (Remel), UVT media (Becton Dickinson), or UTM media (Copan)). Samples were refrigerated for transport at 2-8° C., and stored at that temperature for up to approximately 72 hours before processing. Samples which needed longer storage were stored at ≤−70° C. Nucleic acids were extracted from samples using standard laboratory methods to isolate nucleic acids (e.g., MagNA Pure LC System using the Total Nucleic Acid Isolation Kit (Roche) or the NucliSENS easyMAG System using the Automated Magnetic Extraction Reagents (bioMrieux)). A positive control sample is included which has a target sequence for each of the tested viral strains. That is, if the swine H1N1 influenza A virus is to be tested, a swine H1N1 influenza A virus target sequence is included. Here, the positive controls were pooled RNA transcript from a portion of an HA gene or of an NP gene for each of the subtypes of influenza. In addition, a negative control including the viral transport medium, but not including a target sequence, and Internal Controls were extracted alongside the samples.

US11879161B2. Compositions and assays to detect swine H1N1 influenza a virus nucleic acids (2024-01-23). Gen-Probe Prodesse, Inc. [US]. Inventors: Ejan Tyler [US].

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Bacterial DNA Isolation from Swabs

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Swabs in UTM™ media (Copan Diagnostics) were vortexed for 1 minute on maximum speed. Bacterial DNA was isolated from 1 ml media using PowerSoil® DNA Isolation Kit (MoBio) according to the manufacturer´s protocol. The method was modified by using double amounts of solutions C2, C3 and C4 and a 2h incubation with OB Protease (Peqlab) at 50°C, followed by a homogenization step prior to the first centrifugation step. A negative extraction control was run for each extraction.

Graspeuntner S., Bohlmann M.K., Gillmann K., Speer R., Kuenzel S., Mark H., Hoellen F., Lettau R., Griesinger G., König I.R., Baines J.F, & Rupp J. (2018). Microbiota-based analysis reveals specific bacterial traits and a novel strategy for the diagnosis of infectious infertility. PLoS ONE, 13(1), e0191047.

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Isolation and Characterization of COVID-19 Strains

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Viral strains were isolated from nasopharyngeal swabs obtained from patients suffering from respiratory infection, suspected of COVID-19 and submitted to molecular diagnosis. Nasopharyngeal flocked swabs were suspended in UTM media (Copan, Italy), and kept at 4 °C for less than 48 h. A volume of 400 µl was homogenized and microfiltered (0.5 µm) previous to inoculation on Vero cells CCL-81 (passage 32, from ATCC, USA) grown at 80% confluence in a BSL3 virology laboratory (Virology Unit, CHU de Caen, France). Supernatants were harvested at day 3 after inoculation and immediately used in subsequent passage one (P1) of the virus following the same protocol. P1 was used for stock production and each stock was aliquoted and conserved at −80 °C before titration and genomic quantification. Two strains were used in the model : UCN1 and UCN19, isolated at one week interval during the course of the active epidemic in Normandy, France (end of march).

Bryche B., St Albin A., Murri S., Lacôte S., Pulido C., Ar Gouilh M., Lesellier S., Servat A., Wasniewski M., Picard-Meyer E., Monchatre-Leroy E., Volmer R., Rampin O., Le Goffic R., Marianneau P, & Meunier N. (2020). Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters. Brain, Behavior, and Immunity, 89, 579-586.

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COVID-19 Viral Isolation and Propagation

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Viral strains were isolated from nasopharyngeal swabs obtained from patients suffering from respiratory infection, suspected of COVID-19 and submitted to molecular diagnosis.
Nasopharyngeal flocked swabs were suspended in UTM media (Copan, Italy), and kept at 4°C for less than 48 hours. A volume of 400 µl was homogenized and microfiltered (0.5 µm) previous to inoculation on Vero cells CCL-81 (passage 32, from ATCC, USA) grown at 80% confluence in a BSL3 virology laboratory (Virology Unit, CHU de Caen, France). Supernatants were harvested at day 3 after inoculation and immediately used in subsequent passage one (P1) of the virus following the same protocol. P1 was used for stock production and each stock was aliquoted and conserved at -80°C before titration and genomic quantification. Two strains were used in the model : UCN1 and UCN19, isolated at one week interval during the course of the active epidemic in Normandy, France (end of march).

Bryche B., Albin A.S., Murri S., Lacôte S., Pulido C., Gouilh M.A., Lesellier S., Servat A., Wasniewski M., Picard-Meyer E., Monchâtre-Leroy É., Volmer R., Rampin O., Goffic R.L., Marianneau P., & Meunier N. (2020). Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters.

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