The antibodies anti-53BP1 (ab175933, 1:200 dilution), anti-RPA (ab2175, 1: 200 dilution), RAD51 (ab63801, 1:1000 dilution) and DNA2 (ab96488, 1:1000 dilution) were purchased from Abcam, USA. Antibodies anti-γH2AX (2577, 1:800 dilution), GAPDH (5174, 1:1000 dilution) were purchased from Cell Signaling, USA. BRCA1 (sc-6954) was purchased from Santa Cruz Biotechnology. Antibodies anti-SMARCAL1 (A301-616A, 1:1000 dilution), RPA2-pS4/S8 (A300-245A, 1:200 dilution), ZRANB3 (A303-033A, 1:1000 dilution) was purchased from Bethyl. BRCA2 (OP95, 1:500 dilution) was from Milipore. Antibodies specific for FEN1 (NB100-150, 1:1000 dilution), WRN (NBP1-31,895, 1:1000 dilution), HLTF (NB100-280, 1:2000 dilution) were purchased from Novus Biologicals (USA). Mouse anti-BrdU (347,580, 1:40) was purchased from BD Biosciences. AF647 (A-21247, 1:1000) and AF488 (A-11001, 1:1000) were purchased from ThermoFisher Scientific. DNA-PKcs inhibitor: NU-7441 (HY-11006) were purchased from MedChem Express (USA). MRE11 inhibitor: mirin (M9948) was purchased from Sigma-Aldrich.
Nb100 150
Manufactured by Novus Biologicals
Sourced in United States
The NB100-150 is a laboratory equipment product designed for general laboratory use. It is a centrifuge that can be used to separate and isolate different components of a liquid sample through the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Cell Signaling Technology (2 mentions)
Brca1 sc 6954, by Santa Cruz Biotechnology (1 mentions)
Anti γh2ax 2577, by Cell Signaling Technology (1 mentions)
Af647 a 21247, by Thermo Fisher Scientific (1 mentions)
Af488 a 11001, by Thermo Fisher Scientific (1 mentions)
Anti 53bp1 ab175933, by Abcam (1 mentions)
Rad51 ab63801, by Abcam (1 mentions)
Ab32351, by Abcam (1 mentions)
Ab32042, by Abcam (1 mentions)
Ab182733, by Abcam (1 mentions)
2 protocols using nb100 150
1
DNA Damage Response Protein Analysis
2
DNA Damage Response Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The anti-DNA-PKcs antibody was purchased from Thermo Fisher Scientific Inc. (A303-967A, USA; 1:2000 dilution). Antibodies specific for ATR (#2853), phospho-ATR (Thr1989) (#58014), CHK1 (#2360), phospho-CHK1 (Ser317) (#2344), phospho-p53 (Ser20) (#9287), p53 (#9282), and GAPDH (#5174) were purchased from Cell Signaling and 1:1000 diluted for use. Antibodies specific for YY1AP1 (NBP1-81763, 1:1000 dilution), YY1 (NBP1-46218, 1:2000 dilution), and FEN1 (NB100-150, 1:1000 dilution) were purchased from Novus Biologicals (USA). Antibodies specific for caspase-3 (ab32351, 1:2000 dilution), cleaved-caspase-3 (ab32042, 1:500 dilution), and Bax (ab182733, 1:2000 dilution) were purchased from Abcam (USA). DNA-PKcs inhibitor: VX-984 (HY-19939S) and NU-7441 (HY-11006) were purchased from MedChemExpress (USA).
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