Dsred mice

Manufactured by Jackson ImmunoResearch

DsRed mice are genetically modified mice that express the DsRed fluorescent protein. The DsRed protein is derived from the sea coral Discosoma and emits a red fluorescent signal. These mice are used as a tool in various research applications where a red fluorescent marker is needed.

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Lab products found in correlation

Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Balb c mice, by Jackson ImmunoResearch (1 mentions) Cd45.1 mice, by Jackson ImmunoResearch (1 mentions) Csfr1cre mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j wt mice, by Jackson ImmunoResearch (1 mentions)

3 protocols using dsred mice

Murine Model for ICAM-1 and ICAM-2 Deficiency

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C57BL/6J wild-type mice (000664; JAX) and DsRed mice (006051; JAX; wild-type C57BL/6J background) were obtained from the Jackson Laboratory. Mice were fed a standard rodent chow diet and were housed in microisolator cages in a pathogen-free facility. Mice were euthanized by CO₂ inhalation. All experiments followed guidelines of the La Jolla Institute for Allergy and Immunology Animal Care and Use Committee, and approval for use of rodents was obtained from the La Jolla Institute for Allergy and Immunology according to criteria outlined in the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health.
ICAM-1^null and ICAM-2^−/− mice were described before51 (link)52 (link). They were backcrossed to the C57BL/6 background for at least eight generations64 (link). ICAM-1 and ICAM-2 double-deficient (ICAM-1^null/ ICAM-2^−/−) C57BL/6 mice were generated by crossing ICAM-1^null and ICAM-2^−/− C57BL/6 mice in the specific pathogen-free animal facility, University of Bern, Switzerland. Animal procedures were performed in accordance with the Swiss legislation on the protection of animals and approved by the veterinary office of the Kanton of Bern.

Fan Z., McArdle S., Marki A., Mikulski Z., Gutierrez E., Engelhardt B., Deutsch U., Ginsberg M., Groisman A, & Ley K. (2016). Neutrophil recruitment limited by high-affinity bent β2 integrin binding ligand in cis. Nature Communications, 7, 12658.

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Humane Euthanasia Protocol for Mice

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Six-to-eight-week-old BALB/c mice and DsRed mice were obtained from Jackson Laboratory (Bar Harbor, ME). Animals were housed in barrier facilities at the Duke Cancer Center Isolation Facility (Durham, NC). All mice were maintained in an animal facility accredited by the Association for Assessment and Accreditation of Laboratory Animal Care, and all experimental procedures were performed in accordance with federal and state government guidelines and established institutional guidelines and protocols approved by the Institutional Animal Care and Use Committee at Duke University. Mice were euthanized when humane end points were met. The humane end points included poor posture or ambulating difficulty (e.g., tense, tucked-up, stiff gait), lost hair coat condition (e.g., ruffled fur, lack of grooming, piloerection), sudden activity level change (e.g., restlessness, pacing, reluctance to move), signs of moderate to severe pain or distress, and more than 30% weight loss.

Sung A.D., Yen R.C., Jiao Y., Bernanke A., Lewis D.A., Miller S.E., Li Z., Ross J.R., Artica A., Piryani S., Zhou D., Liu Y., Vo-Dinh T., Hoffman M., Ortel T.L., Chao N.J, & Chen B.J. (2020). Fibrinogen-Coated Albumin Nanospheres Prevent Thrombocytopenia-Related Bleeding. Radiation research, 194(2), 162-172.

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Mouse Models for Immunological Studies

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C57BL/6J WT mice (000664; JAX), DsRed mice (006051; JAX; WT C57BL/6J background), CD45.1 mice (002014; JAX; WT C57BL/6J background), CFTR^ΔF508 or CF mice (002515; JAX; C57BL/6J background), and CSFR1-cre mice (029206; JAX; C57BL/6J background) were obtained from the Jackson Laboratory. CFTR^flox/flox (Cftr^tm1Cwr; C57BL/6J background) mice were developed by and obtained from the Cystic Fibrosis Mouse Models Core at Case Western Reserve University. CD45.1/CD45.2 mice were bred by C57BL/6J WT and CD45.1 mice. Mice were fed a standard rodent chow diet and were housed in microisolator cages in a pathogen-free facility in the La Jolla Institute for Immunology or UConn Health. Mice were euthanized by CO₂ inhalation. All experiments followed guidelines of the La Jolla Institute for Immunology and UConn Health Animal Care and Use Committee, and approval for the use of rodents was obtained from the La Jolla Institute for Immunology and UConn Health according to criteria outlined in the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health.

Fan Z., Pitmon E., Wen L., Miller J., Ehinger E., Herro R., Liu W., Chen J., Mikulski Z., Conrad D.J., Marki A., Orecchioni M., Kumari P., Zhu Y.P., Marcovecchio P.M., Hedrick C.C., Hodges C.A., Rathinam V.A., Wang K, & Ley K. (2022). Bone marrow transplantation rescues monocyte recruitment defect and improves cystic fibrosis in mice. Journal of immunology (Baltimore, Md. : 1950), 208(3), 745-752.

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